久效磷对美国红鱼鳃Na+/K+-ATP酶活性和超显微结构的影响

应用生态学报 ›› 2003, Vol. ›› Issue (12): 2289-2294.

久效磷对美国红鱼鳃Na⁺/K⁺-ATP酶活性和超显微结构的影响

魏渲辉, 汝少国, 姜明, 李永祺

中国海洋大学海洋生命学院, 青岛 266003

收稿日期:2002-05-04 修回日期:2002-11-06 出版日期:2003-12-15
通讯作者: 汝少国
基金资助:
国家自然科学基金项目(39870580);教育部留学回国人员科研启动基金资助项目

Effect of monocrotophos on Na⁺/K⁺-ATP ase activity and ultramicrostructure of Sciaenops ocellatus branchiae

WEI Xuanhui, RU Shaoguo, JIANG Ming, LI Yongqi

College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China

Received:2002-05-04 Revised:2002-11-06 Online:2003-12-15

摘要/Abstract

摘要： 经不同试验浓度的久效磷(0.25、0.5、1.0和2.0mg·L^-1)处理美国红鱼4d后,分别对鱼鳃Na⁺/K⁺ATP酶活性和氯细胞密度进行了测定和计数,并观察了鱼鳃组织显微结构和超微结构的变化.结果表明,低浓度久效磷(0.25mg·L^-1)处理可以诱导鱼鳃氯细胞大量增生,Na⁺/K⁺-ATP酶活性增强,随着试验浓度的增加,久效磷对鳃组织的损伤越来越重,Na⁺/K⁺-ATP酶活性逐渐降低;久效磷对鱼鳃显微结构的损伤表现为鳃小片上皮细胞水肿、脱离,鳃小片基部粘连,鳃小片上皮细胞角质化;超微结构变化主要为内质网、线粒体、微小管和核膜的水肿及部分溶解,这种损伤表现为由细胞膜到细胞核的动态过程.

Abstract: The exposure of Sciaenops ocellatus to monocrotophos at its concentration of 0.25,0.5,1.0 or 2.0 mg稬^-1 for 4 days demonstrated that under the stress of low concentration (0.25 mg稬^-1) monocrotophos,the chloride cells of branchiae proliferated,accompanied with the increase of Na⁺/K⁺-ATP ase activity,while under high concentrations of monocrotophos,the damage of gill was much heavier,and the Na⁺/K⁺-ATP ase activity also decreased. The damage in gill under sublethal monocrotophos exposure included hypertrophy and edema,and thus,the lamellar epithelium became thick and lifting,and the lamellae were bulbing or fusing. The ultramicrostructural changes of the gill of the exposure fish were the swollen and even rupture of rER,mitochondria,microtubule and nuclear membrane.

中图分类号:

X592

魏渲辉, 汝少国, 姜明, 李永祺. 久效磷对美国红鱼鳃Na⁺/K⁺-ATP酶活性和超显微结构的影响[J]. 应用生态学报, 2003, (12): 2289-2294.

WEI Xuanhui, RU Shaoguo, JIANG Ming, LI Yongqi . Effect of monocrotophos on Na⁺/K⁺-ATP ase activity and ultramicrostructure of Sciaenops ocellatus branchiae[J]. Chinese Journal of Applied Ecology, 2003, (12): 2289-2294.

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