铁胁迫诱导的赤潮异弯藻细胞生化组成变化

应用生态学报 ›› 2003, Vol. ›› Issue (7): 1185-1187.

铁胁迫诱导的赤潮异弯藻细胞生化组成变化

李东侠¹, 丛威¹, 蔡昭铃¹, 施定基², 欧阳藩¹

1. 中国科学院过程工程研究所生化工程国家重点实验室, 北京 100080;
2. 中国科学院植物研究所光合作用研究中心, 北京 100093

收稿日期:2002-12-18 修回日期:2003-02-25 出版日期:2003-07-15
通讯作者: 丛威
基金资助:
国家重点基础研究发展规划资助项目(2001CB409706).

Induction of biochemical composition in Heterosigma akashiwo under Fe stress

LI Dongxia¹, CONG Wei¹, CAI Zhaoling¹, SHI Dingji², OUYANG Fan ¹

1. National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China;
2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2002-12-18 Revised:2003-02-25 Online:2003-07-15

摘要/Abstract

摘要： 研究了铁胁迫对赤潮异弯藻细胞生化组成的影响.结果表明,铁胁迫下的细胞内所有色素浓度均降低,同富铁条件相比,铁胁迫下的细胞内叶绿素a浓度降低2倍多,叶绿素c也有相当程度的降低,因此,铁胁迫下的胞内叶绿素c与叶绿素a的比率变化不大.铁胁迫下的细胞内类胡萝卜素的含量降低了1.5倍,因此在铁胁迫下的细胞内类胡萝卜素相对于叶绿素a的比例升高,碳水化合物含量随培养基内铁浓度降低而下降,与铁丰富条件(10μmol·L^-1)相比,受铁胁迫的细胞可溶蛋白电泳图谱中17kDa和55kDa附近的带明显增加,而在20kDa和35kDa附近的蛋白带降低.

Abstract: The iron-stress mediated effects on biochemical constituents of red tide alga H. akashiwo were examined in Fe-replete and iron deficiency,and low iron batch cultures. The content of all pigments decreased under iron limitation,and the cellular chlorophyll a concentration decreased by more than 2-fold compared to that under iron replete condition. This change in Chl a was accompanied by a corresponding decrease in Chl c per cell, resulting in no trend in the ratio of Chl c to Chl a. The cellular carotenoids content decreased by more than1.5-fold compared to that under iron replete condition. As a consequence, carotenoids/chlorophyll a ratio increased in Fe-deficient cells. Carbohydrate content was reduced under iron stress,and total proteins decreased with the decrease of iron concentration. Acrude fractionation of the soluble proteins demonstrated that 17 kDand 55 kDa proteins in soluble fraction were induced.

中图分类号:

Q178.53

李东侠, 丛威, 蔡昭铃, 施定基, 欧阳藩. 铁胁迫诱导的赤潮异弯藻细胞生化组成变化[J]. 应用生态学报, 2003, (7): 1185-1187.

LI Dongxia, CONG Wei, CAI Zhaoling, SHI Dingji, OUYANG Fan . Induction of biochemical composition in Heterosigma akashiwo under Fe stress[J]. Chinese Journal of Applied Ecology, 2003, (7): 1185-1187.

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