基因工程微生物生态学研究进展

应用生态学报 ›› 2003, Vol. ›› Issue (2): 293-295.

基因工程微生物生态学研究进展

靳素英¹, 张嘉治², 王颜红¹, 孟书颖³

1. 中国科学院沈阳应用生态研究所, 沈阳 110016;
2. 沈阳农业大学, 沈阳 1101613;
3. 沈阳市产品质量监督检验所, 沈阳 110015

收稿日期:2002-10-31 修回日期:2002-12-05 出版日期:2003-02-15
通讯作者: 张嘉治
基金资助:
中国科学院陆地生态过程重点实验室基金资助项目(K09WK10980911)

Advances in studies on genetically engineered microorganism ecology

JIN Suying¹, ZHANG Jiazhi², WANG Yanhong¹, MENG Shuying³

1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
2. Shenyang Agricultural University, Shenyang 110161, China;
3. Institute of Monitor and Checkout of Production in Shenyang, Shenyang 110015, China

Received:2002-10-31 Revised:2002-12-05 Online:2003-02-15

摘要/Abstract

摘要： 基因工程微生物(genetically engineered microorganism,GEM)生态学的研究已成为微生物分子生态学的一项主要研究内容之一.随着分子标记和分子生物学检测手段的引入,传统的微生物生态学研究被注入了新的活力,在分子水平上探讨基因工程微生物与环境及环境中土著生物之间的关系已成为可能.基因工程微生物生态学是一门内容涉及分子生物学、微生物学、生态学等诸多学科的新型交叉边缘学科.本文提出加紧进行转基因生物生态学和转基因生物的风险评价的研究工作,建立适合中国国情的检测手段和评价标准,有助于我国基因工程微生物生态学的健康发展.

Abstract: Genetically engineered microorganism ecology has been one of main research contents in microorganism molecular ecology. Along with the introduction of molecular marker and molecular biology,traditional microorganism ecology has been developed; therefore, it is possible to study the relationship between GEM and environment, environmental microorganism under molecular level. The GEM ecology has become a new and intersection borderline discipline, related to molecular biology, microbiology, ecology and so on. Moreover, it brought forward that the prosecution of the research on the transgenic organism ecology and the risk assessment, and the foundation of checking means and valuating standard that adapt to the situation of China could help to the development of GEM ecology in China.

中图分类号:

Q938.1

靳素英, 张嘉治, 王颜红, 孟书颖. 基因工程微生物生态学研究进展[J]. 应用生态学报, 2003, (2): 293-295.

JIN Suying, ZHANG Jiazhi, WANG Yanhong, MENG Shuying. Advances in studies on genetically engineered microorganism ecology[J]. Chinese Journal of Applied Ecology, 2003, (2): 293-295.

参考文献

[1] Atlas RM.1992.Detection and enumeration of microorganisms based upon phenotype In:Levin MA,Seidler RJ,Ouied MR eds.Microbial Ecology.New York:McGraw Hill,Inc.29～43
[2] Bernard L,Courties C,Duperray C,et al.2001.A new approach to determine the genetic diversity of viable and active bacteria in aquatic ecosystems.Cytometry,43(4) :314～321
[3] Colwell RK,Tiedje JM,et al.1989.The planted introduction of genetically engineered organisms:Ecological consideration and recommendations.Ecology, 70:298～315
[4] Cui X-M(崔学明),Zhang C-G(张成刚) .1996 .Use of a xylE marker gene to monitor the survival of rhizobia popuations in soil.Chin J Appl Ecol(应用生态学报),7(3):287～298(in Chinese)
[5] Devanas,BD,Stosky G.1986.Fate in soil of a recombinant plasmid carrying a Drosophila gene.Curr Microbiol,13:279～283
[6] Henschke RB, Schmidt FRJ.1990.Plasmid mobilization from genetically engineered bacteria to members of the indigenous soil microflora in situ.Curr Microbiol,20:105～110
[7] Kapustka LA,Williams BL,Fairbrother A.1996.Evaluation risk predictions at population and community levels in pesticide registration-hypotheses to be tested.Environ Toxic Chem,15(4):427～431
[8] Kinkle KB,Schmidt ED.1991.Transfer of symbiotic plasmid pJB5JI in nonsterile soil.Appl Environ Microbiol,57:3264～3269
[9] Levine MM,Kaper JB,Lockman H,et al.1983.Recombinant DNA risk assessment studies in humans:Efficacy of poorly mobilizablie plasmids in biological continent.J Infect Dis,148:699～709
[10] Li G-Q(李国旗),An S-Q(安树清),Chen X-L(陈兴龙).1999 .A summary on ecological risk assessment.Chin J Ecol(生态学杂志),18(4):57～64(in Chinese)
[11] Lin J-S(蔺继尚),Cui X-M(崔学明).1994.Transposon Tn5 used as an identifiable ecological marker of R.fredii stains:Horizontal transfer frequency of transposon Tn5 and its influence on the movement of R.fredii Tn5 mutant.Chin J Appl Ecol(应用生态学),5(3):292～298(in Chinese)
[12] Lin J-S(蔺继尚),Cui X-M(崔学明),Jin S-Y(靳素英),et al.1995.Impact of high temperature stress on survival of Tn5 mutants of Rhizobium fredii and phenotypic expression of Tn5 in soil.Chin J Appl Ecol(应用生态学报),6(4):411～416(in Chinese)
[13] Lorenzota MG.1991.Gene transfer via transformation in soil sediment environments.In:Ganthier MJ ed.Gene Transfers and Environment.Berlin :Springer-Verlag Inc.94～101
[14] Ma X-D(马向东),Cheng Q-L(成庆利),Kang H-X(康海霞).2001.Research advances in microbial transformation techniques.Henan Agric Univ(河南农业大学学报),35(4):299～303(in Chinese)
[15] Natarajan MR,Orie LP.1992.Transfer of transposon Tn916 from Bacillus subtilis into a natural soil population.Appl Environ Microbiol,58:2710～2730
[16] Prosser JI.1994.Molecular marker systems for detection of genetically engineered microorganisms in the environment.Microbiology,140:5～17
[17] HRartl SD,Pepper IL .1991.Transposon Tn5 as an identifiable marker in Rhizobia.Microbial Ecol,21:21～23
[18] Sayre P, Miller RV.1991.Bacterial mobile genetic elements:Importance in assessing the environmental fate of genetically engineered sequences.Plasmid,26:151～171
[19] Shaw JJ,Dane F,Geiger D,Kloepper JW.1992.Use of bioluminescence for detection of genetically engineered microorganisms released into the environment.Appl Environ Microbiol,58:267～273
[20] Smit E, Elass JD, Veen JA.1992. Risks associated with the application of genetically modified microorganisms in terrestrial ecosystems.FEMS Microbiol Rev,88:263～278
[21] Steffan RJ,Atlas RM.1988.DNA amplification to enhance detection of genetically engineered bacteria in environmental samples.Appl Environ Microbiol,54:2185～2191
[22] Svayer GS,Sheilds MS,Tedford ET .1995 .Application of DNA-DNA colony hybridization to the detection of catabolic genotypes in environmental samples.Appl Environ Microbiol,49:1295～1303
[23] Tiedje JM,Colwell RK,Grosman YL,et al.1989.The planted introduction of genetically engineered organisms:Ecological consideration and recommendations.Ecology,70:298～315
[24] Wilson M,Lindow SE.1993.Release of recombinant microorganisms.Ann Rev Microbiol,47:913～944
[25] Xiao D-N(肖笃宁),Chen W-B(陈文波),Guo F-L(郭福良).2002. On the basic concepts and contents of ecological security.Chin J Appl Ecol(应用生态学报),13(3):354～358(in Chinese)
[26] Zhang S-Q(张素琴),Luo R-X(罗如新).1999.Microbial genetic adaptability to environment and molecular ecological efficiency of plasmid.Chin J Appl Ecol(应用生态学报),10(4):506～510(in Chinese)
[27] Zhang S-T(张尚通),Xu C-R (许崇任).1994. Ecology of genetically engineered microorganisms and risk assessment for their planned release.Chin J Appl Ecol(应用生态学报),5(3):325～330(in Chinese)
[28] Zhong M(钟鸣),Zhou Q-X(周启星).2002. Molecular-ecological technology of microorganisms and its application to research on environmental pollution.Chin J Appl Ecol(应用生态学报),13(2):247～251(in Chinese)