Hydrogen sulfide acted as a downstream signal was involved in the regulation of salicylic acid on photosynthesis of cucumber seedlings under low temperature and low light intensity

doi:10.13287/j.1001-9332.202009.028

Abstract

Abstract: Both salicylic acid (SA) and hydrogen sulfide (H₂S) play an important role in regulating plant growth and development and physiological metabolism under abiotic stresses. As signal molecules, the interaction between them in regulating cucumber photosynthesis under low temperature and low light is still unclear. Here, we examined the regulation and interaction of SA and H₂S on photosynthesis in cucumber seedlings under low temperature (8 ℃/5 ℃, day/night) and low light (100 μmol·m^-2·s^-1). Seedlings were foliar-sprayed with SA, sodium sulfide (NaHS, H₂S donor), and their scavenger or biosynthesis inhibitors, respectively. Seedlings treated with deionized water at suitable temperature and light condition were used as the control. The results showed that SA increased the L-/D-cysteine desulfhydrase (LCD, DCD) activities and relative mRNA expression, and consequently promoted the endogenous H₂S production. However, NaHSdid not affect the activities and gene expressions of phenylalnine ammonialyase and isochorismate and endogenous SA level. Compared with the H₂O-treated seedlings under low temperature and low light, SA- and NaHS-treated seedlings showed an increase in the photosynthetic rate, stomatal conductance and transpiration rate, while a decrease in intercellular CO₂ concentration. SA and NaHS increased the CO₂ assimilation, which mainly attributed to the increases in the activities of the ribulose-1, 5-bisphosphate carboxylase, rubisco activase, sedoheptulose-1, 7-bisphosphatase and fructose-1, 6-bisphosphatase, as well as their mRNA expression. Meanwhile, SA and NaHS improved the actual photochemical efficiency and maximal photochemical efficiency of PSII, and therefore alleviated the damage in photosynthetic apparatus and negative effect on growth from low temperature and low light stress. The SA-induced higher photosynthesis and growth in stressed seedlings were suppressed by addition of H₂S scavenger hypotaurine. However, the H₂S-induced tolerance of photosynthetic apparatus to low temperature and low light was not affected by SA biosynthesis inhibitor paclobutrazol and 2-aminoindan-2-phosphonic acid. Our results suggested that H₂S, as a downstream signal of SA, was involved in regulating photosynthesis in cucumber seedlings under low temperature and low light.

Key words: photosynthesis, chilling-induced photoinhibition, salicylic acid, hydrogen sulfide, cucumber

PAN Dong-yun, FU Xin, ZHANG Xiao-wei, LIU Feng-jiao, BI Huan-gai, AI Xi-zhen. Hydrogen sulfide acted as a downstream signal was involved in the regulation of salicylic acid on photosynthesis of cucumber seedlings under low temperature and low light intensity[J]. Chinese Journal of Applied Ecology, 2020, 31(9): 3023-3032.

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