Effects of addition of sorghum stubble rhizosphere soil on growth and rhizosphere microbes of continuous cropping cucumber

doi:10.13287/j.1001-9332.202109.032

Abstract

Abstract: We explored the effects of addition of sorghum stubble rhizosphere soil on the growth of continuous cropping cucumber and rhizosphere microbial community in a pot experiment. The diffe-rences in soil bacterial and fungal community composition were analyzed with fluorescence quantitative PCR and high-throughput sequencing technology. There were four treatments: CK (no fertilization), T₁(fertilizer only), T₂(optimized fertilization), and T₃(optimized fertilization + rhizosphere soil of sorghum stubble). The results showed that compared with other treatments, T₃ promoted the growth and development of cucumber, and increased the abundance of 16S rRNA and ITS rRNA genes in soil. Compared with the T₁ treatment, T₂ and T₃ significantly increased the richness and diversity of bacterial communities. There was no significant difference in fungal community richness and diversity among different treatments. Adding rhizosphere soil of sorghum stubble changed the composition of bacterial and fungal communities at both phylum and genus levels. For bacteria, it increased the abundances of Acidobacteria and Bacteroides, but decreased that of Proteobacteria, Firmicutes, Nitrospira and Bacillus. For fungi, it increased the abundance of Basidiomycota, Trichoderma and Pseudurotium, but decreased that of Fusarium and Metarhizium. Results of redundancy analysis showed that soil nitrate and organic matter were the key factors affecting the difference of bacterial and fungal community composition, respectively. In conclusion, addition of sorghum stubble rhizosphere soil improved the total abundance of soil microorganisms and bacterial diversity for continuous cropping cucumber. It increased the abundance of beneficial bacteria Trichoderma, reduced that of pathogenic Fusarium, and maintained the survival rate of cucumber, thus provided a feasible solution for alleviating the barriers for the continuous cropping of cucumber.

Key words: bacteria, fungi, microbial community, continuous cropping obstacle

ZHANG Lei, ZHOU Zhe-zhe, WANG Xue-xia, WANG Jia-chen, WANG Dian-wu, CHEN Yan-hua. Effects of addition of sorghum stubble rhizosphere soil on growth and rhizosphere microbes of continuous cropping cucumber[J]. Chinese Journal of Applied Ecology, 2021, 32(9): 3240-3248.

References

[1] 沈宗专, 孙莉, 王东升, 等. 石灰碳铵熏蒸与施用生物有机肥对连作黄瓜和西瓜枯萎病及生物量的影响. 应用生态学报, 2017, 28(10): 3351-3359 [Shen Z-Z, Sun L, Wang D-S, et al. Effects of lime-ammonium bicarbonate fumigation and biofertilizer application on Fusarium wilt and biomass of continuous cropping cucumber and watermelon. Chinese Journal of Applied Ecology, 2017, 28(10): 3351-3359]
[2] Elad Y, Cytryn E, Harel YM, et al. The biochar effect: Plant resistance to biotic stresses. Phytopathologia Mediterranea, 2011, 50: 335-349
[3] Bonanomi G, Lorito M, Vinale F, et al. Organic amendments, beneficial microbes, and soil microbiota: Toward a unified framework for disease suppression. Annual Review of Phytopathology, 2018, 56: 1-20
[4] 姚春芝, 蒋宇婷, 杨玉婷, 等. 三七连作土壤浸提液对其根腐病菌的化感效应. 应用生态学报, 2020, 31(7): 2227-2235 [Yao C-Z, Jiang Y-T, Yang Y-T, et al. Allelopathic effect of extracts from panax notoginseng monocropped soil on its root rot pathogens. Chinese Journal of Applied Ecology, 2020, 31(7): 2227-2235]
[5] Li S, Wu FZ. Diversity and cooccurrence patterns of soil bacterial and fungal communities in seven intercropping systems. Frontiers in Microbiology, 2018, 9: 1521
[6] Wolińska A, Kuźniar A, Zielenkiewicz U, et al. Indicators of arable soils fatigue-bacterial families and genera: A metagenomic approach. Ecological Indicators, 2018, 93: 490-500
[7] 曲成闯, 陈效民, 张志龙, 等. 施用生物有机肥对黄瓜连作土壤有机碳库和酶活性的持续影响. 应用生态学报, 2019, 30(9): 3147-3154 [Qu C-C, Chen X-M, Zhang Z-L, et al. Long-term effects of bioorganic fertilizer application on soil organic carbon pool and enzyme activity of cucumber continuous cropping. Chinese Journal of Applied Ecology, 2019, 30(9): 3147-3154]
[8] Jiang YJ, Liang YT, Li CM, et al. Crop rotations alter bacterial and fungal diversity in paddy soils across East Asia. Soil Biology and Biochemistry, 2016, 95: 250-261
[9] 王彩云, 武春成, 曹霞, 等. 生物炭对温室黄瓜不同连作年限土壤养分和微生物群落多样性的影响. 应用生态学报, 2019, 30(4): 1359-1366 [Wang C-Y, Wu C-C, Cao X, et al. Effects of biochar on soil nutrition and microbial community diversity under continuous cultivated cucumber soils in greenhouse. Chinese Journal of Applied Ecology, 2019, 30(4): 1359-1366]
[10] 王劲松, 樊芳芳, 郭珺, 等. 不同作物轮作对连作高粱生长及其根际土壤环境的影响. 应用生态学报, 2016, 27(7): 2283-2291 [Wang J-S, Fan F-F, Guo J, et al. Effects of different crop rotations on growth of continuous cropping sorghum and its rhizosphere soil micro-environment. Chinese Journal of Applied Ecology, 2016, 27(7): 2283-2291]
[11] Luis LB, Rafael LB, Purificación FG, et al. Carbon storage in a rainfed mediterranean vertisol: Effects of tillage and crop rotation in a long-term experiment. European Journal of Soil Science, 2020, 71: 472-483
[12] Schmidt JH, Bergkvist G, Campiglia E, et al. Effect of tillage subsidiary crops and fertilisation on plant parasitic nematodes in a range of agro-environmental conditions within Europe. Annals of Applied Biology, 2017, 171: 477-489
[13] Ugo DC, Luigi P, Nicola A, et al. Soil management under tomato-wheat rotation increases the suppressive response against Fusarium wilt and tomato shoot growth by changing the microbial composition and chemical parameters. Applied Soil Ecology, 2020, 154, doi: 10.1016/j.apsoil.2020.103601
[14] Zhou XG, Liu J, Wu FZ. Soil microbial communities in cucumber monoculture and rotation systems and their feedback effects on cucumber seedling growth. Plant and Soil, 2017, 415: 507-520
[15] Gianinazzi S, Gollotte A, Binet MN, et al. Agroecology: The key role of arbuscular mycorrhizas in ecosystem services. Mycorrhiza, 2010, 20: 519-530
[16] 石兆勇, 李珂, 王发园, 等. 纳米银和外源丛枝菌根真菌对甜高粱叶绿素荧光诱导动力学特性的影响. 浙江农业学报, 2020, 32(2): 283-290 [Shi Z-Y, Li K, Wang F-Y, et al. Effects of nano-silver and exotic arbuscular mycorrhizal fungi on chlorophyll fluorescence kinetics of sweet sorghum. Acta Agriculturae Zhejiangensis, 2020, 32(2): 283-290]
[17] Subbarao GV, Nakahara K, Ishikawa T, et al. Free fatty acids from the pasture grass brachiaria humidicolaand one of their methyl esters as inhibitors of nitrification. Plant and Soil, 2008, 313: 89-99
[18] Arya M, Shergil IS, Williamson M, et al. Basic principles of real-time quantitative PCR. Expert Review of Molecular Diagnostics, 2005, 5: 209-219
[19] Lievens B, Claes L, Vanachter ACRC, et al. Detecting single nucleotide polymorphisms using DNA arrays for plant pathogen diagnosis. FEMS Microbiology Letters, 2006, 255: 129-139
[20] Jia BH, Wang YY, Xie ZH. Responses of the terrestrial carbon cycle to drought over China: Modeling sensitivities of the interactive nitrogen and dynamic vegetation. Ecological Modelling, 2018, 368: 52-68
[21] Liu SH, Xu JQ, Wei H, et al. Effect of garlic straw on physical and chemical characteristics of continuous cropping soil and root activity of tomato in solar greenhouse. Agricultural Science & Technology, 2016, 17: 1349-1354
[22] Namdas DD, Bhosale AM, Khilare CJ. Rhizosphere and soil mycoflora of sorghum and tomato growing at Ahmednagar (M.S). Bioinfolet-A Quarterly Journal of Life Sciences, 2009, 6: 244-245
[23] Teixeira H, Rodríguez-Echeverría S. Identification of symbiotic nitrogen-fixing bacteria from three African leguminous trees in Gorongosa National Park. Systematic and Applied Microbiology, 2016, 39: 350-358
[24] 何志刚, 董环, 娄春荣, 等. 玉米秸秆对设施次生盐渍化土壤微环境及番茄生长的影响. 中国蔬菜, 2019(6): 39-44 [He Z-G, Dong H, Lou C-R, et al. Effects of corn stalks on microenvironment of facility secondary salinized soil and tomato growth. China Vegetables, 2019(6): 39-44]
[25] 王廷峰, 赵密珍, 关玲, 等. 玉米套作及秸秆还田对草莓连作土壤养分及微生物区系的影响. 江苏农业学报, 2019, 35(6): 1421-1427 [Wang T-F, Zhao M-Z, Guan L, et al. Effects of intercropping with corn and straw returning on nutrients and microflora in strawberry continuous cropping soil. Jiangsu Journal of Agricultural Sciences, 2019, 35(6): 1421-1427]
[26] Xu AX, Li LL, Coulter JA, et al. Long-term nitrogen fertilization impacts on soil bacteria, grain yield and nitrogen use efficiency of wheat in semiarid loess plateau. Agronomy, 2020, 10, doi: 10.3390/agronomy10081175
[27] Chen JH, Wu QF, Li SH, et al. Diversity and function of soil bacterial communities in response to long-term intensive management in a subtropical bamboo forest. Geoderma, 2019, 354, doi: 10.1016/j.geoderma.2019.113894
[28] Su Y, Yu M, Xi H, et al. Soil microbial community shifts with long-term of different straw return in wheat-corn rotation system. Scientific Reports, 2020, 10: 291-301
[29] Sandra G, Kristin K, Bernd W, et al. The effects of cropping regimes on fungal and bacterial communities of wheat and faba bean in a greenhouse pot experiment differ between plant species and compartment. Frontiers in Microbiology, 2017, 8: 902, doi: 10.3389/fmicb.2017.00902
[30] 赵雅姣, 刘晓静, 吴勇, 等. 西北半干旱区紫花苜蓿-小黑麦间作对根际土壤养分和细菌群落的影响. 应用生态学报, 2020, 31(5): 1645-1652 [Zhao Y-J, Liu X-J, Wu Y, et al. Effects of medicago sativa-triticale wittmack intercropping system on rhizosphere soil nutrients and bacterial community in semi-arid region of Northwest China. Chinese Journal of Applied Ecology, 2020, 31(5): 1645-1652]
[31] Arshad A, Paula DM, Jeroen F, et al. Mimicking microbial interactions under nitrate-reducing conditions in an anoxic bioreactor: Enrichment of novel nitrospirae bacteria distantly related to thermodesulfovibrio. Environmental Microbiology, 2017, 19: 4965-4977
[32] Guo MS, Ding GD, Gao GL, et al. Community composition of ectomycorrhizal fungi associated with pinus sylvestris var. mongolica plantations of various ages in the horqin sandy land. Ecological Indicators, 2020, 110, doi: 10.1016/j.ecolind.2019.105860
[33] 杨立宾, 隋心, 魏丹, 等. 大兴安岭棕色针叶林土壤的真菌多样性. 应用生态学报, 2019, 30(10): 3411-3418 [Yang L-B, Sui X, Wei D, et al. Fungal diversity in the brown coniferous forest soils of Daxing’anling Mountains, Northeast China. Chinese Journal of Applied Ecology, 2019, 30(10): 3411-3418]
[34] Beimforde C, Feldberg K, Nylinder S, et al. Estimating the phanerozoic history of the Ascomycota lineages: Combining fossil and molecular data. Molecular Phylogenetics and Evolution, 2014, 78: 386-398
[35] Gadhi MA, Nizamani ZA, Jatoi GH, et al. In-vitro efficacy of bio-control agent and essential oils against leaf blight of chickpea caused by Alternaria alternata. Acta Ecologica Sinica, 2020, 40: 166-171
[36] Himaman W, Thamchaipenet A, Pathom-aree W, et al. Actinomycetes from eucalyptus and their biological activities for controlling eucalyptus leaf and shoot blight. Microbiological Research, 2016, 188: 42-52
[37] 姚薇, 曲明星, 崔晓慧, 等. 木霉菌合成银纳米粒子条件的优化及其对甜瓜尖孢镰刀菌抑制作用. 生物工程学报, 2020, 36(9): 1859-1868 [Yao W, Qu M-X, Cui X-H, et al. Optimization of synthesizing silver nanoparticles from Trichoderma strains for inhibition of Fusarium oxysporum. Chinese Journal of Biotechnology, 2020, 36(9): 1859-1868]
[38] 张晶, 于玲玲, 辛术贞, 等. 根茬连续还田对镉污染农田土壤中镉赋存形态和生物有效性的影响. 环境科学, 2013, 34(2): 685-691 [Zhang J, Yu L-L, Xin S-Z, et al. Effects of continuous cropping of stubble on the occurrence form and bioavailability of cadmium in cadmium-polluted farmland soil. Environmental Science, 2013, 34(2): 685-691]
[39] 李增亮, 任晶, 梁鑫宇, 等. 七种植物秸秆对连作番茄苗生长及土壤酶活性的影响. 北方园艺, 2019(20): 17-24 [Li Z-L, Ren J, Liang X-Y, et al. Effects of seven plant straws on the growth and soil enzyme activity of tomato seedlings. Northern Horticulture, 2019(20): 17-24]
[40] Zhao J, Ni T, Li J, et al. Effects of organic-inorganic compound fertilizer with reduced chemical fertilizer application on crop yields, soil biological activity and bacterial community structure in a rice-wheat cropping system. Applied Soil Ecology, 2016, 99: 1-12
[41] 刘平静, 肖杰, 孙本华, 等. 长期不同施肥措施下土娄土细菌群落结构变化及其主要影响因素. 植物营养与肥料学报, 2020, 26(2): 307-315 [Liu P-J, Xiao J, Sun B-H, et al. Variation of bacterial community structure and the main influencing factors in eum-orthic anthrosols under different fertilization regimes. Journal of Plant Nutrition and Fertilizers, 2020, 26(2): 307-315]
[42] 朱杰, 刘海, 吴邦魁, 等. 稻虾共作对稻田土壤nirK反硝化微生物群落结构和多样性的影响. 中国生态农业学报, 2018, 26(9): 1324-1332 [Zhu J, Liu H, Wu B-K, et al. Effects of integrated rice-crayfish farming system on community structure and diversity of nirK denitrification microbe in paddy soils. Chinese Journal of Eco-Agriculture, 2018, 26(9): 1324-1332]
[43] 王悦, 杨贝贝, 王浩, 等. 不同种植模式下丹参根际土壤微生物群落结构变化. 生态学报, 2019, 39(13): 4832-4843 [Wang Y, Yang B-B, Wang H, et al. Variation in microbial community structure in the rhizosphere soil of salvia miltiorrhiza bunge under three cropping modes. Acta Ecologica Sinica, 2019, 39(13): 4832-4843]