Analytical method of polycyclic aromatic hydrocarbons (PAHs) in soil and plant samples

Abstract

Abstract: Soil, plant and seed samples are respectively extracted by tetrahydrafuran, methanol andethyl acetate ultrasonically.The extracts are evaporated by rotary evaporator, cleaned upon silica gel column and analyzed on HPLCwith fluorescence detector.The recovery ofPAHs is 45.68-93.42% for soil, 77.59-108.13% for plant, and 79.11-98.96% forseed, depending on the physico-chemical properties of individual PAHcompounds.Dichloromethane and tetrahydrafuran are the suitable extracting agents for soil, andmethanol and ethyl acetate for plant and seed.respectively.This method has the characteristics of simplicity, rapidity, sensitivity and reliance, and can be used for the study ofPAHs in soil-plant system.

Key words: Polycyclic aromatic hydrocarbons, HPLC Soil and plant analysis, vegetation restoration, soil microbial biomass carbon, soil physical-chemical property, growth stage

Song Yufang, Ou Ziqing, Sun Tieheng, A. Yediler, G. Lorinci, A. Kettrup. Analytical method of polycyclic aromatic hydrocarbons (PAHs) in soil and plant samples[J]. Chinese Journal of Applied Ecology, 1995, 6(1): 92-96.

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