Abstract: Under field condition with 16～18℃ water temperature,single-factor acute toxicity test was used to study the toxicity effects of water pH,salinity and carbonate-alkalinity on abnormal tadpole (R.chensinensis).The results showed that when the water salinity was 0.18 g·L^-1,carbonate-alkalinity was 1.41 mmol·L^-1,and water pH was 4.3～9.7,the survival rate of abnormal tadpole within 96 hours was not affected.The upper limit of LC₅₀ for the pH within 24,48,72 and 96 hours was 10.33,10.18,10.08 and 10.02,and the prescribed minimum was 3.92,4.07,4.11 and 4.16,respectively.The upper limit of LC₀ was 9.95,9.80,9.70 and 9.70,and the prescribed minimum was 4.23,4.45,4.30 and 4.30,and that of LC₁₀₀ was 10.70,10.55,10.45 and 10.33,and the prescribed minimum was 3.55,3.70,3.92 and 4.03,respectively.The survival rate of abnormal tadpole within 96 hours was not affected in the water salinity between 2.0～3.0 g·L^-1.When water pH was 7.0～8.5 and carbonate-alkalinity was 1.41mmol·L^-1,the LC₅₀ of the salinity within 24,48,72 and 96 hours was 8.21,7.25,5.17 and 3.70 g·L^-1,the LC₀ was 7.14,6.00,2.67 and 2.20 g·L^-1,and the LC₁₀₀ was 9.98,9.00,7.67 and 5.20 g·L^-1,respectively,while the SC was 1.70 g·L^-1.Under the same water pH and when the water salinity was 0.18 g·L^-1,the LC₅₀ of carbonate-alkalinity within 24,48,72 and 96 hours was 14.36,11.83,10.35,and 7.68 mmol·L^-1,the LC₀ was 8.76,8.51,4.65 and 3.88 mmol·L^-1,and the LC₁₀₀ was 19.96,15.14,16.05 and 11.48 mmol·L^-1,respectively,while the SC was 1.70 mmol·L^-1.The survival rate of abnormal tadpole (R.chensinensis) was decreased with increasing water pH,salinity and carbonate-alkalinity.The optimum water salinity and carbonate-alkalinity to the survival and the growth of abnormal tadpole (R.chensinensis) were below 2.0 g·L^-1 and 3.0 mmol·L^-1,respectively,and water pH was between 6.0 and 9.0.

Key words: Rana chensinensis, Abnormal tadpole, pH, Salinity, Alkalinity, Adaptability