Effects of Cu2O nanoparticles on the kinetic characteristics of rapid chlorophyll fluorescence induction and related genes in wheat seedlings

doi:10.13287/j.1001-9332.202403.009

Abstract

Abstract: Metal nanoparticles could be accumulated in soils, which threatens the ecological stability of crops. Investigating the effects of cuprous oxide nanoparticles (Cu₂O-NPs) on photosystem Ⅱ (PSⅡ) of wheat seedling leaves holds considerable importance in comprehending the implications of Cu₂O-NPs on crop photosynthesis. Following the hydroponic method, we investigated the effects of 0, 10, 50, 100, and 200 mg·L^-1 Cu₂O-NPs on chlorophyll fluorescence induction kinetics and photosynthetic-related genes in wheat seedlings of “Zhoumai 18”. The results showed that, with the increases of Cu₂O-NPs concentrations, chlorophyll contents in wheat leaves decreased, and the standardization of the OJIP curve showed a clearly K-phase (ΔK＞0). Cu₂O-NPs stress increased the parameters of active PSⅡ reaction centers, including the absorption flux per active RC (ABS/RC), the trapping flux per active RC (TRo/RC), the electron transport flux per active RC (ETo/RC), and the dissipation flux per active RC (DIo/RC). Cu₂O-NPs stress decreased the parameters of PSⅡ energy distribution ratio including the maximum quantum yield of PSⅡ (φ_Po), the quantum yield of electron transport from QA (φ_Eo), and the probability that a trapped exciton moved an electron further than QA (Ψ_o), while increased the quantum ratio for heat dissipation (φ_Do). Moreover, there was a decrease in photosynthetic quantum yield Y(Ⅱ), photochemical quenching coefficient (q_P), net photosynthetic rate (P_n), stomatal conductance (g_s), intercellular CO₂ concentration (C_i), and transpiration rate (T_r) of leaves with the increases of Cu₂O-NPs concentration. Under Cu₂O-NPs stress, the expression levels of genes which included PSⅡ genes (PsbD, PsbP, Lhcb1), Rubisco large subunit genes (RbcL), cytochrome b6/f complex genes (PetD, Rieske), and ATP synthase genes (AtpA, AtpB, AtpE, AtpI) were downregulated. These results indicated that Cu₂O-NPs stress altered the activity and structure of PSⅡ in wheat seedlings, affected the activity of PSⅡ reaction centers, performance parameters of PSⅡ donor and acceptor sides. PSⅡ related genes were downregulated and exhibited significant concentration effects.

Key words: wheat, cuprous oxide nanoparticles, chlorophyll fluorescence, photosystem Ⅱ

GUO Wei, WANG Nan, ZHANG Kaiyue, SONG Peipei, MA Zhanqiang. Effects of Cu₂O nanoparticles on the kinetic characteristics of rapid chlorophyll fluorescence induction and related genes in wheat seedlings[J]. Chinese Journal of Applied Ecology, 2024, 35(3): 721-730.

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Effects of Cu₂O nanoparticles on the kinetic characteristics of rapid chlorophyll fluorescence induction and related genes in wheat seedlings

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