Relationships between antioxidant activities and heat-resistant features of two Laminaria japonica strains

Abstract

Abstract: The study on the relationships between antioxidant activities and heat-resistant features of two Laminaria japonica gametophyte mutants Kelp 901(abbreviated as 901)and Rongcheng No.1(abbreviated as RC)under different temperature conditions showed that under normal temperature condition(10 ),there were no positive relationships between the basal antioxidant activities and heat-resistant features of 901 and RC,while under heat stress(18 ),the reactive oxygen species(ROS)contents kept constant in 901,but accumulated rapidly in RC,suggesting that ROS played an essential role in oxidative stress.The enzyme activities in RC were more vulnerable to high temperature,and its antioxidant enzyme activities decreased more sharply under heat stress.Ascorbic acid(ASA),vitamin E,superoxide dismutase(SOD)and glutathione peroxidase(Gpx)might play important roles in kelp's heat-resistance.Chlorophyll a was sensitive to heat stress,and its 50% nocuity time could be used as an indicator to distinguish kelp strains with different heat-resistance.It was suggested that the antioxidant system's responses to heat stress instead of its basic activities accounted for the kelp's heat-resistance.

Key words: Laminaria japonica, Strain, Antioxidant system, Heat-resistance, Relationship

CLC Number:

X171.5

WANG You, TANG Xuexi . Relationships between antioxidant activities and heat-resistant features of two Laminaria japonica strains[J]. Chinese Journal of Applied Ecology, 2005, 16(8): 1507-1512.

References

[1] Aebi H.1984.Catalase in vitio.Method Enzymol,105:121～130
[2] Arriqoni O,Garal DE,Tommasi F,et al.1992.Changes in the ascorbate system during seed development of Viciafaba L.Plant Physiol,99:235～238
[3] Beauchamp C,Fridovich I.1971.Superoxide dismutases:Improved assays and an assay applicable to acrylamide gels.Anal Biochem,44:276～287
[4] Bewley C.1979.Physiological aspects of desiccation tolerance.Rev Plant Physiol,30:195～238
[5] Bowler C,Montagv MV.1992.Superoxide dismutase and stress tolerance.An n Rev Plant Physiol Plant Mol Biol,43:83～116
[6] Bradford MM.1976.A rapid and sensitivity method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem,72:248～254
[7] Chen H-Y(陈火英),Wang L-Z(汪隆植),Zhang J-H(张建华).1992.Analyzing techniques on heat-resistance of turnip.Shanghai Veg(上海蔬菜),3:15～16(in Chinese)
[8] Chen S-Y(陈少裕).1991.Injury of membrane lipid peroxidation to plant cell.Plant Physiol Commun(植物生理学通讯),27(2):84～90(in Chinese)
[9] Chu L(储玲),Liu D-Y(刘登义),Wang Y-B(王友保),et al.2004.Effect of copper pollution on seedling growth and activate oxygen metabolism of Trifolium pretense.Chin J Appl Ecol(应用生态学报),15(1):119～122(in Chinese)
[10] Dat JF,Lopez-Delgado H.1998.Parallel changes in H2O₂ and catalase during thermotolerance by salicylic acid and heat acclimation in mustard seedlings.Plant Physiol,116:1351～1357
[11] Desei ID.1984.Vitamin E analysis methods for animal tissues.Meth Enzymol,105:138～147
[12] Diguiseppi J,Fridovich I.1984.The toxicology of molecular oxygen.CRC Crit Rev Toxicol,12:315～342
[13] Du Y-J(杜英君),Jin Y-H(靳月华).2000.Effect of far-ultraviolet radiation on lipid peroxidation and inheritent protection system in seedlings of Taxus cuxpidata.Chin J Appl Ecol(应用生态学报),11(5):660～664(in Chinese)
[14] Evans JJ.1965.The distribution of peroxide in extreme dwarfs and normal tomato.Phytochemistry,(4):499～503
[15] Fujita S,Nazamid S.1995.Purification and properties of polyphenol oxidase from cabbage(Brassica olerace L.).J Agric Food Chem,43(50):1138～1142
[16] Gong M,Luit AH.1998.Heat-shock induced changes intercellular Ca2+ level in tobacco seedlings in relation to thermotolerance.Plant Physiol,116:429～437
[17] Guo X-R(郭晓荣),Cao K-F(曹坤芳),Xu Z-F(许再富).2004.Response of photosynthesis and antioxygenic enzymes in seedlings of three tropical forest tree species to different light environments.Chin J Appl Ecol(应用生态学报),15(3):377～381(in Chinese)
[18] Halliwell B.1992.Reactive oxygen species and the central nervous system.J Neurochem,59:1609～1623
[19] He X-M(何晓明),Lin Y-E(林毓娥),Chen Q-H(陈清华),et al.2002.Effect of heat stress on seedling growth,praline contents and SOD activity in cucumbers.J Shanghai Jiaotong Univ(Agric Sci)(上海交通大学学报·农业科学版),20(1):30～33(in Chinese)
[20] HuangJ(黄健),TangX-X(唐学玺),LiuT(刘涛),et al.2002.Alteration of activated oxygen and antioxidant system in kelp during alginic acid decomposing bacteria infection.J Ocean Univ Qingdao(青岛海洋大学学报),32(4):574～578(in Chinese)
[21] Huang J(黄健),Tang X-X(唐学玺),Liu T(刘涛),et al.2002.Alteration of ultrastructures and levels of polyphenol and polyphenoloxidase of Laminaria japonica during alginic acid decomposing bacteria infection.High Technol Commun(高技术通讯),9:74～77(in Chinese)
[22] Ishii S.1987.Generation of active oxygen species during enzymatic isolation of protoplast from oat leaves.In vitro,23(4):653～657
[23] Jenssen A.1978.Chlorophyll a and Carotenoids.In:Handbook of Physiological and Biochemical Method.New York:Cambridge University Press.59～70
[24] Jones PD.1985.Northern hemisphere surface air temperature variations:1851～1984.J Clim Appl Meteorol,25:161～179
[25] Liu F(刘峰),Zhang J(张军),Zhang W-J(张文吉),et al.2004.Physiological mechanism of ride seedlings in low temperature and damping-off disease resistance induced by calcium application into soil.Chin J Appl Ecol(应用生态学报),15(5):763～766(in Chinese)
[26] Lopez DH,Dat JF,Foyer C,et al.1998.Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O₂.J Exp Bot,49(321):713～720
[27] Mcister A.1991.Glutathione deficiency produced by inhibition of its synthesis,and its reversal:Applications in research and therapy.Pharmacol Therap,51:155～194
[28] Mercedes J,Josefa EC.1998.Oxidation of the flavonol fisetin by polyphenol oxidase.Biochem Biophysic Acta,1425:534～542
[29] Pierpoint WS.1994.Salicylic acid and its derivatives in plants:Medicines,metabolites and messenger moleculars.Adv Bot Res,20:165～235
[30] Priti Krishna.2003.Plant responses to heat stress.Topics Curr Genets,4:73～101
[31] Qing W-Y(强维亚),Yang H(杨晖),Chen T(陈拓),et al.2004.Effect of the combination of cadmium and UV-B radiation on soybean growth.Chin J Appl Ecol(应用生态学报),15(4):697～700(in Chinese)
[32] Rong Z-X(荣征星),Liu H-Z(刘慧中).1994.The micro assay of the glutathione peroxidation activity in whole blood of mouse.Prog Biochem Bioph ysiol(生物化学与生物物理学进展),21(4):362～366(in Chinese)
[33] Sies H.1993.Strategies of antioxidant defense.Eur J Biochem,215:213～219
[34] Song H-Y(宋洪元),Lei J-J(雷建军),Li C-Q(李成琼).1998.Response to heat stress and identification,assessment of heat resistance in plants.Chin Vege(中国蔬菜),1:48～50(in Chinese)
[35] Tanake KY,Suda N.1985.O₃ tolerance and ascorbate-dependent H2O₂ decomposing system in chloroplasts.Plant Cell Physiol,26:1425～1431
[36] Tang X-X(唐学玺),Yan T-J(颜挺进),Li Y-Q(李永祺).1998.Damage effect of monocrotophos on Platymonas sp.I.Active oxygen in Platymonas sp.cells.Chin J Appl Ecol(应用生态学报),9(6):627～630(in Chinese)
[37] Tang X-X(唐学玺),Yang Z(杨震),Huang J(黄健),et al.2001.Effects of infection by alginic acid decomposing bacteria on activity of antioxidant enzymes in Laminaria japonica.J Fish Chin(水产学报),25(5):424～427(in Chinese)
[38] Xue Y-L(薛应龙).1985.The Experimental Handbook of Plant Physiology.Shanghai:Shanghai Science and Technology Press.(in Chinese)
[39] Yan X-J(严小军).1996.Analysis of polyphenol contents in popular brown alga of China.Stu Mar Sin(海洋科学集刊),37:61～65(in Chinese)
[40] Zhang Q-S(张全胜),Liu S-P(刘升平).2001.Studies on rearing new variety of kelp 901 transactions.Chin J Ocean Limnol(海洋湖沼通报),2:46～53(in Chinese)
[41] Zhou L(周丽),Gong Q-L(宫庆礼),Yu K-K(俞开康),et al.1996.Disease of kelp,Laminaria japonica.Chin J Ocean Limnol(海洋湖沼通报),(4):38～43(in Chinese)
[42] Zhou Y-H(周艳虹),Yu J-Q(喻景权),Qian Q-Q(钱琼秋),et al.2003.Effects of chilling and low light on cucumber seedlings growth and their antioxidative enzyme activities.Chin J Appl Ecol(应用生态学报),14(6):921～924(in Chinese)
[43] Zou Q(邹琦).1988.Heat injury and heat acclimation of wheat.Acta Bot Sin(植物学报),30(4):388～395(in Chinese)