Physiological responses of Ceratophyllum demersum under different nutritional conditions

Abstract

Abstract: With static experiments,this paper investigated the protein and chlorophyll contents,and the peroxidase (POD) and superoxide dismutase (SOD) activities of Ceratophyllum demersum cultured in meso-,eutro-and hypertrophic and Hoagland's solutions.The results showed that the changes of total N (TN) and total P (TP) concentrations in nutrient solutions had an obvious effect on the biosynthesis of C.demersum.When the concentrations of TN and TP were less than 1 mg稬^-1 and 0.1 mg稬^-1,respectively,the protein and chlorophyll contents of plant declined.The plant from eutrophic solution had higher SOD and POD activities.It was indicated that C.demersum was more preferable to eutropic condition (1 mg TN稬^-1 and 0.1 mg TP稬^-1),and the raised concentration of nutrients (e.g.,hypertrophic and Hoagland's solutions) was a stress to C.demersum and could affect its hardiness physiology.

Key words: Ceratophyllum demersum, Physiological response, Eutrophication, Stress

CLC Number:

X524

WANG Jun, GU Yufei, Zhu Zengyin, WU Bin, YIN Daqiang . Physiological responses of Ceratophyllum demersum under different nutritional conditions[J]. Chinese Journal of Applied Ecology, 2005, 16(2): 337-340.

References

[1] Barnes, JD, Balaguer L, Manrique E, et al. 1992. A reappraisal of the use of DMSO for the extraction and determination of chlorophyll a and b in lichens and higher plants. Environ Exp Bot, 32:85～100
[2] Chen S-Q(陈毓荃). 1995. Reserch Technique of Biochemistry.Beijing:China Agriculture Press. 86 (in Chinese)
[3] Chen H-S(陈荷生). 2001. Eutrophication of Taihu and improvement for N and P pollution. Hydro Water Resour(水文水资源),22(3):17～32(in Chinese)
[4] Chen S-Y(陈少裕). 1991. Injury of membrane lipid peroxidation to plant cell. Plant Physiol Commun (植物生理学通讯), 27(2):84～90(in Chinese)
[5] Deng B-Y(邓碧玉), Yuan Q-S(袁勤生), Li W-J(李文杰). 1991.The improved method to mensurate superoxide dismutase activity by benzene-1, 2, 3-triol oxidation. Prog Biochem Biophys (生物化学和生物物理进展), 18(2):163(in Chinese)
[6] Huang Q-P (黄漪平). 2001. Water Environment and Pollution Control of Taihu lake. Beijing:Science Press. 61～65(in Chinese)
[7] Lehmann A, Lachavanne JB. 1999. Changes in the water quality of Lake Geneva indicated by submerged macrophytes. Freshwater Biol, 42:457～466
[8] Li Y-S(李阳生), Li S-Q(李绍清). 2000. Effect of submergence stress on physiological indexes and yield component at reproductive stage in rice. J Wuhan Bot Res(武汉植物学研究), 18(2):117～122(in Chinese)
[9] Quan W-M(全为民), Yah L-J(严力蛟), Yu Z-M(虞左明).2001. Advance in study of lake eutrophication models. Biodiv Sci (生物多样性), 9(2):168～175(in Chinese)
[10] Ren N(任南),Yan G-A(严国安),Ma J-M(马剑敏),et al.1996. The study on the influence of environmental factors of the submerged macrophytes. J Wuhan Univ (Nat Sci) (武汉大学学报·自然科学版),42(2):213～218(in Chinese)
[11] Schmieder K. 1997. Littoral zone-GIS of lake constance:A Useful tool in lake monitoring and autecological studies with submerged macrophytes. Aquatic Bot, 58:333～346
[12] Shen Y-F(沈韫芬), Zhang Z-S(章宗涉). 1999. Water pollution biology. In:Liu J-K(刘建康), ed. Advanced Hydrobiology. Beijing:Science Press. 305～338(in Chinese)
[13] Shu X-S(舒孝顺), Chen L-B(陈良碧). 1999. The activity of peroxidase in the thermo sensitive genic male sterile rice during fertility sensitive period. Plant Physiol Commun (植物生理学通讯), 35(6):466～468 (in Chinese)
[14] Siesko MM, WJ Fleming, RM Grossfeld. 1997. Stress protein synthesis and peroxidease activity in a submersed aquatic macrophyte exposed to cadmium. Environ Toxicol Chem, 16(8):1755～1760
[15] Wang B(王斌), Li W(李伟). 2002. Physiological reactions of Potamogeton malaianus to different N and P concentrations in the growth medium. Acta Ecol Sin (生态学报), 22(10):1620(in Chinese)
[16] Wang H-Z(王海珍), Chen D-H(陈德辉), Wang Q-X(王全喜).2001. The effect of aquatic vegetation on ecological restoration of eutrophication lake. Nature J (自然杂志), 24 (1):33～36 (in Chinese)
[17] Yang Q-X(杨清心). 1998. Ecological functions of aquatic vegetation in East Taihu Lake and its reasonable regulation. J Lake Sci (湖泊科学), 10(1):67～72(in Chinese)
[18] Yuan J-F(袁峻峰), Zhang Z-S(章宗涉). 1993. Biochemical interference of aquatic macrophyte Ceratophyllum demersum on algae.Acta Ecol Sin(生态学报), 13(1):45～50(in Chinese)
[19] Yu S-D(俞是聃). 2002. Determination of total nitrogen and total phosphorous in the same clearing up solution at the same time.Arid Environ Mon(干旱环境监测), 16(3):133～134 (in Chinese)
[20] ZhouJ-Y(周军英),Dai Z-K(戴珍科),Wu Z-L(武志林). 1993.The effects of SO₂ + NO₂ on superoxide dismutase activity and leaf injury of tomato plant. China Environ Sci (中国环境科学), 13(6):429 (in Chinese)