钨酸钠对苹果幼苗15N吸收利用、13C积累及果实品质的影响

doi:10.13287/j.1001-9332.202001.024

应用生态学报 ›› 2020, Vol. 31 ›› Issue (1): 182-188.doi: 10.13287/j.1001-9332.202001.024

钨酸钠对苹果幼苗¹⁵N吸收利用、¹³C积累及果实品质的影响

王芬, 岳玉金, 李敏, 罗家坤, 葛顺峰, 姜远茂^*

山东农业大学园艺科学与工程学院/作物生物学国家重点实验室, 山东泰安 271018

收稿日期:2019-03-07 出版日期:2020-01-15 发布日期:2020-01-15
通讯作者: E-mail: ymjiang@sdau.edu.cn
作者简介:王芬, 女, 1992年生, 博士研究生。主要从事苹果氮素营养研究。E-mail: 757086004@qq.com
基金资助:
国家重点研发计划项目(2016YFD0201100)、国家自然科学基金项目(31501713)、国家现代农业产业技术体系建设资金项目(CARS-27)和山东省泰山学者攀登计划

Effects of Na₂WO₄ on ¹⁵N absorption and utilization,¹³C accumulation of apple seedling, and fruit quality

WANG Fen, YUE Yu-jin, LI Min, LUO Jia-kun, GE Shun-feng, JIANG Yuan-mao^*

College of Horticulture Science and Engineering, Shandong Agricultural University/ State Key Laboratory of Crop Biology, Tai’an 271018, Shandong, China

Received:2019-03-07 Online:2020-01-15 Published:2020-01-15
Contact: E-mail: ymjiang@sdau.edu.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2016YFD0201100), the National Natural Science Foundation of China (31501713), the China Modern Agriculture Industry System Construction Foundation (CARS-27) and the Taishan Scholar Assistance Program of Shandong Province.

摘要/Abstract

摘要： 分别以1年生苹果砧木M9T337幼苗和5年生烟富3/SH6/平邑甜茶为试材,开展盆栽和田间试验,并结合¹⁵N、¹³C同位素示踪技术,研究不同浓度(0、0.5、1、1.5 mmol·L^-1,分别用CK、T₁、T₂和T₃表示)硝酸还原酶(NR)抑制剂钨酸钠对苹果幼苗¹⁵N吸收利用、¹³C积累和成熟期果实品质的影响。结果表明: 盆栽试验中,喷施0.5~1.0 mmol·L^-1钨酸钠可显著抑制幼苗地上部生长,但对根系生长影响不显著;当钨酸钠浓度达到1.5 mmol·L^-1时可显著抑制根系生长。同一时期各处理叶片NR活性与钨酸钠浓度呈负相关,均表现为CK>T₁>T₂>T₃。随处理时间的延长,叶片硝态氮含量总体表现为先升高后降低的趋势,同一时期各处理硝态氮含量与钨酸钠浓度呈正相关,均表现为T₃>T₂>T₁>CK。喷施钨酸钠可不同程度地降低幼苗各器官¹⁵N吸收量和¹⁵N利用率,且钨酸钠浓度越高,抑制幼苗氮素吸收和利用的效果越显著。随钨酸钠浓度的提高,地上部¹³C积累量呈先升高后降低的趋势,在T₂处理时达到最高;幼苗整株¹³C积累量呈相似的规律。田间试验结果表明,喷施钨酸钠可降低成熟期叶片和果实的氮含量,果皮花青苷含量、果实可溶性固形物、可溶性糖含量和糖酸比均不同程度提高,其中T₂处理的效果最好。综上,T₂处理(1.0 mmol·L^-1钨酸钠)可有效抑制幼苗地上部生长,降低¹⁵N吸收利用,提高¹³C积累,有利于果实品质的提高。

Abstract: Pot culture and field experiments were carried out with one-year-old apple rootstock M9T337 seedlings and five-year-old ‘Yanfu3’/SH6/M. hupehensis Rehd. as test materials respectively. Combined with ¹⁵N and ¹³C isotope tracer technology, we examined the effects of different concentrations of NR inhibitor Na₂WO₄(0, 0.5, 1, 1.5 mmol·L^-1, expressed by CK, T₁, T₂ and T₃ respectively) on ¹⁵N absorption and ¹³C accumulation of seedling and apple quality. The results showed that 0.5-1.0 mmol·L^-1 Na₂WO₄ significantly inhibited shoot growth but not root growth of seedling in the pot experiment. Root growth was significantly inhibited when the concentration of Na₂WO₄ reached 1.5 mmol·L^-1. The NR activity of each treatment was negatively correlated with the concentrations of Na₂WO₄ in the same period, with an order of CK>T₁>T₂>T₃. The content of nitrate in leaves showed the trend of first rising and then decreasing with the extension of processing time. Nitrate content was positively correlated with Na₂WO₄ concentration in the same period, with an order of T₃>T₂>T₁>CK. Spraying Na₂WO₄ reduced the ¹⁵N absorption of each organ and ¹⁵N utilization rate, with such effects increasing with the amount of spraying. With the increases of Na₂WO₄ concentration, ¹³C accumulation on the ground increased first and then decreased, with highest value in T₂ treatment. The ¹³C accumulation of whole plant showed a similar pattern. The results of field experiment showed that Na₂WO₄ application reduced nitrogen contents of leaves and fruits at maturity stage, and increased the anthocyanin content in peel, soluble solids, soluble sugar content and sugar acid ratio in fruits. The T₂ treatment showed the best effect. In summary, T₂ treatment (1.0 mmol·L^-1 Na₂WO₄) could inhibit shoot growth of seedlings, reduce ¹⁵N absorption and utilization, improve ¹³C accumulation, which would improve apple quality.

王芬, 岳玉金, 李敏, 罗家坤, 葛顺峰, 姜远茂. 钨酸钠对苹果幼苗¹⁵N吸收利用、¹³C积累及果实品质的影响[J]. 应用生态学报, 2020, 31(1): 182-188.

WANG Fen, YUE Yu-jin, LI Min, LUO Jia-kun, GE Shun-feng, JIANG Yuan-mao. Effects of Na₂WO₄ on ¹⁵N absorption and utilization,¹³C accumulation of apple seedling, and fruit quality[J]. Chinese Journal of Applied Ecology, 2020, 31(1): 182-188.

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钨酸钠对苹果幼苗¹⁵N吸收利用、¹³C积累及果实品质的影响

Effects of Na₂WO₄ on ¹⁵N absorption and utilization,¹³C accumulation of apple seedling, and fruit quality

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