硫化氢对盐碱胁迫下裸燕麦叶片抗坏血酸-谷胱甘肽循环的调控效应

doi:10.13287/j.1001-9332.202111.023

摘要/Abstract

摘要： 盐碱胁迫是植物遭受的常见非生物胁迫之一,气体信号硫化氢(H₂S)在植物响应盐碱胁迫中发挥着重要作用。为探讨H₂S对盐碱胁迫下裸燕麦抗坏血酸(AsA)-谷胱甘肽(GSH)循环的调控效应,以品种‘定莜9号’为材料,研究了喷施H₂S供体硫氢化钠(NaHS)或H₂S合成抑制剂羟胺(HA)对盐碱混合胁迫下植株生长、叶片活性氧、膜脂过氧化和AsA-GSH循环中抗氧化物质和关键酶的影响。结果表明: 喷施50 μmol·L^-1 NaHS可缓解50 mmol·L^-1盐碱混合胁迫对裸燕麦生长的抑制,降低超氧阴离子、H₂O₂、丙二醛、氧化型抗坏血酸(DHA)、还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)含量,提高AsA/DHA和GSH/GSSG,而对还原型抗坏血酸(AsA)含量无显著影响。喷施NaHS还提高了盐碱混合胁迫下裸燕麦叶片AsA合成关键酶L-半乳糖脱氢酶(GalDH)和L-半乳糖-1,4-内酯脱氢酶(GalLDH)及AsA-GSH循环中单脱氢抗坏血酸还原酶(MDHAR)活性,降低了抗坏血酸过氧化物酶(APX)和脱氢抗坏血酸还原酶(DHAR)活性,而对抗坏血酸氧化酶(AO)和谷胱甘肽还原酶(GR)活性的影响不大。增添HA后部分或完全解除了喷施NaHS的上述作用。这说明H₂S可通过促进AsA合成和增强MDHAR活性提高AsA-GSH循环效率,降低盐碱胁迫对裸燕麦的氧化伤害。

关键词: 硫化氢, 盐碱胁迫, 裸燕麦, AsA-GSH循环

Abstract: Saline-alkali stress is one of the common abiotic stresses for plants. Hydrogen sulfide (H₂S), as a gas signal, plays an important role in driving the responses of plants to saline-alkali stress. To explore the regulating effects of H₂S on the ascorbate (AsA)-glutathione (GSH) cycle in naked oat (Avena nude) under saline-alkali stress, we used sodium hydrogen sulfide (NaHS) as donor of exogenous H₂S and hydroxylamine (HA) as H₂S synthesis inhibitor to examine the effects of H₂S on plant growth, leaf reactive oxygen species, membrane lipid peroxidation, and antioxidants and key enzymes in the AsA-GSH cycle in “Dingyou 9” variety of naked oat under saline-alkali mixed stress. Results showed that spraying 50 μmol·L^-1 NaHS could alleviate the inhibition of 50 mmol·L^-1 saline-alkali mixed stress on the growth of naked oats, reduce the content of superoxide anions, H₂O₂, malondialdehyde, oxidized ascorbate (DHA), glutathione (GSH), and oxidized glutathione (GSSG) in leaves of naked oat under saline-alkali mixed stress, increase the ratio of AsA/DHA and GSH/GSSG, but did not affect the content of reduced ascorbic acid (AsA). Spraying NaHS significantly increased the activities of key enzymes, L-galactose dehydrogenase (GalDH) and L-galactono-1, 4-lactone dehydrogenase (GalLDH), for AsA synthesis pathways in naked oat leaves under salt-alkali mixed stress, as well as monodehydroascorbate reductase (MDHAR) in the AsA-GSH cycle, and decreased the activities of ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR), but did not affect the activities of ascorbate oxidase (AO) and glutathione reductase (GR). The addition of HA partially or completely relieved those aforementioned effects. Our results indicated that H₂S could increase the efficiency of AsA-GSH cycle by promoting the synthesis of AsA and enhancing the activity of MDHAR, and reduce the oxidative damage of saline-alkali stress to naked oats.

Key words: hydrogen sulfide, saline-alkali stress, naked oat, AsA-GSH cycle.

刘建新,刘瑞瑞,贾海燕,刘秀丽,卜婷,李娜. 硫化氢对盐碱胁迫下裸燕麦叶片抗坏血酸-谷胱甘肽循环的调控效应[J]. 应用生态学报, 2021, 32(11): 3988-3996.

LIU Jian-xin, LIU Rui-rui, JIA Hai-yan, LIU Xiu-li, BU Ting, LI Na. Regulation effects of hydrogen sulfide on ascorbate-glutathione cycle in naked oat leaves under saline-alkali stress[J]. Chinese Journal of Applied Ecology, 2021, 32(11): 3988-3996.

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