壳聚糖对NaCl胁迫下菜用大豆叶绿体蛋白的影响

doi:10.13287/j.1001-9332.202201.029

摘要/Abstract

摘要： 壳聚糖(CTS)能有效增强植物对盐胁迫的耐受性,但CTS在蛋白质组水平上对菜用大豆幼苗响应盐胁迫的影响尚不清楚。本研究用200 mmol·L^-1 CTS和蒸馏水分别喷洒菜用大豆‘绿领特早'幼苗叶片,诱导5 d后进行NaCl胁迫和无NaCl胁迫营养液处理,在NaCl处理第3天取样提取幼苗叶片叶绿体蛋白,进行同位素标记相对和绝对定量(iTRAQ)分析。结果表明: CTS显著提高了NaCl胁迫下菜用大豆幼苗的净光合速率(P_n)。试验总计鉴定到549个可靠定量信息叶绿体蛋白,其中有442个至少存在于两次生物学重复蛋白中,26个上调蛋白和4个下调蛋白与CTS影响菜用大豆响应NaCl胁迫有关。分子功能和代谢通路富集分析发现,上调叶绿体蛋白主要与电子转运、叶绿素结合、电子载流子活性等光合作用的分子功能相关,并富集在光反应、碳反应及乙醛酸和二元酸代谢等途径中;下调叶绿体蛋白主要与聚(U) RNA结合有关。上述结果显示,NaCl胁迫下CTS可以通过多种途径影响菜用大豆幼苗的光合作用。

关键词: 外源壳聚糖, NaCl胁迫, 菜用大豆, 叶绿体蛋白质组

Abstract: Chitosan (CTS) can effectively enhance the tolerance of plants to salt stress, but its role in driving the responses of vegetable soybean seedlings to salt stress at proteomic level is still unclear. Here, both 200 mmol·L^-1 CTS and distilled water were used to spray the leaves of vegetable soybean ‘Lvlingtezao' seedlings. After 5 days of induction, NaCl stress and nutrient solution without NaCl were treated. Chloroplast proteins were extracted from leaves on the third day of NaCl treatment and analyzed by using the isobaric tags for relative and absolute quantification (iTRAQ). The result showed that CTS significantly increased net photosynthetic rate (P_n) of vegetable soybean seedlings under NaCl stress. Totally 549 reliable quantitative information proteins were identified, of which 442 existed in at least two biological repeats, including 26 up-regulated proteins and 4 down-regulated proteins associated with the effects of CTS on vegetable soybean response to NaCl stress. In addition, enrichment analysis of molecular function and metabolic pathway showed that up-regulated proteins were mainly related to molecular functions, including electron transport, chlorophyll binding, electron carrier activity, and were enriched in the pathways of photoreaction, carbon reaction and glyoxylic acid and dicarboxylic acid metabolism. Down-regulated proteins were mainly related to poly (U) RNA binding. Our results suggested that CTS could affect photosynthesis of vegetable soybean seedlings under NaCl stress through multiple pathways.

Key words: exogenous chitosan, NaCl stress, vegetable soybean, chloroplast proteome

王聪, 郭园, 张巍巍. 壳聚糖对NaCl胁迫下菜用大豆叶绿体蛋白的影响[J]. 应用生态学报, 2022, 33(1): 111-118.

WANG Cong, GUO Yuan, ZHANG Wei-wei. Effects of chitosan on chloroplast protein of vegetable soybean under NaCl stress[J]. Chinese Journal of Applied Ecology, 2022, 33(1): 111-118.

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