青海地区解磷微生物的筛选及对小油菜生长的影响

doi:10.13287/j.1001-9332.202212.035

应用生态学报 ›› 2023, Vol. 34 ›› Issue (1): 221-228.doi: 10.13287/j.1001-9332.202212.035

青海地区解磷微生物的筛选及对小油菜生长的影响

孙健¹, 王亚艺², 张鑫鹏¹, 李松龄^2*

¹青海大学, 西宁 810016;
²青海大学农林科学院, 西宁 810016

收稿日期:2021-12-13 修回日期:2022-04-26 出版日期:2023-01-15 发布日期:2023-06-15
通讯作者: ^*E-mail: 465883114@qq.com
作者简介:孙健, 男, 1991年生, 硕士。主要从事有机肥研发。E-mail: 1611499005@qq.com
基金资助:
青海省科学技术厅重大科技专项((2019-NK-A11))和青海省中央引导地方科技发展资金项目(2021ZY016)资助。

Screening of phosphorus solubilizing microorganisms in cold environment and their effects on the growth of Brassica napus

SUN Jian¹, WANG Ya-yi², ZHANG Xin-peng¹, LI Song-ling^2*

¹Qinghai University, Xining 810016, China;
²Academy of Agricultural Forestry Sciences, Qinghai University, Xining 810016, China

Received:2021-12-13 Revised:2022-04-26 Online:2023-01-15 Published:2023-06-15

摘要/Abstract

摘要： 为了筛选出能适应青海省冷凉气候环境的解磷菌株,以磷酸钙、卵磷脂、植酸为单一磷源,对胶冻样芽孢杆菌、巨大芽孢杆菌、蜡状芽孢杆菌、紫变异链霉菌、肉桂褐链霉菌、黄团孢链霉菌进行固体平板培养基初筛和液体培养基复筛,通过综合比较固体平板培养基中解磷圈的大小和液体培养基可溶性磷含量,初步筛选出解磷效果较好的3株解磷菌,分别为紫变异链霉菌、肉桂褐链霉菌和胶冻样芽孢杆菌。将这3株解磷菌制成液体菌剂,在9月冷凉气候下采用两种不同肥力土壤进行小油菜盆栽试验。结果表明:与对照相比,施加紫变异链霉菌剂后,高肥力耕地土的小油菜收获时株高、鲜重、根长、根重分别增加了35.5%、191.0%、26.2%、282.7%,植株磷吸收量、根际土壤全磷、速效磷分别增加了968.9%、5.1%、2.1%;低肥力自然土的小油菜收获时株高和鲜重分别增加了45.8%和61.3%,根长和根重分别减少了2.6%和4.4%,植株磷吸收量、根际土壤全磷、速效磷分别增加了91.5%、29.1%和213.7%。其他两种解磷菌剂效果不如紫变异链霉菌明显,表明紫变异链霉菌为适合青海地区冷凉气候环境的解磷菌株。

关键词: 解磷菌, 促生作用, 碱性土壤

Abstract: To screen out phosphorus solubilizing strains that can adapt to cold climate in Qinghai Province, Bacillus mucilaginosus, B. megaterium, B. cereus, Streptomyces violovariabilis, S. cinnamofuscus, and S. flavoagglomeratus were screened with solid plate medium as the primary and liquid medium as the secondary screening, with calcium phosphate, lecithin, and phytic acid as the single source of phosphorus. By comprehensively comparing the size of phosphate solubilizing circle in the solid plate medium and the soluble phosphorus content in the liquid medium, three strains of phosphate solubilizing bacteria with good phosphate solubilizing effects were screened, S. violovariabilis, S. cinnamofuscus, and B. mucilaginosus. The three phosphate solubilizing bacteria were made into liquid ino-culants, and the small rapeseed pot experiment was carried out with two soils with different fertilities in a cold climate in September. Compared with the control, plant height, fresh weight, root length, and root weight of rapes in high-fertility cultivated soil increased by 35.5%, 191.0%, 26.2%, and 282.7%, while plant phosphorus absorption, total phosphorus and available phosphorus contents in the rhizosphere soil increased by 968.9%, 5.1%, and 2.1%, respectively. In low-fertility soil, plant height and fresh weight was increased by 45.8% and 61.3%, root length and weight was decreased by 2.6% and 4.4%, while plant phosphorus absorption and the contents of total P and available P in rhizosphere soil were increased by 91.5 %, 29.1%, and 213.7%, respectively. The effect of the other two inoculants treatments was less significant than S. violovariabilis. Therefore, S. violovariabilis was the phosphate solubilizing strain suitable for the cold climate in Qinghai.

Key words: phosphate solubilizing bacteria, growth promoting effect, alkaline soil.

孙健, 王亚艺, 张鑫鹏, 李松龄. 青海地区解磷微生物的筛选及对小油菜生长的影响[J]. 应用生态学报, 2023, 34(1): 221-228.

SUN Jian, WANG Ya-yi, ZHANG Xin-peng, LI Song-ling. Screening of phosphorus solubilizing microorganisms in cold environment and their effects on the growth of Brassica napus[J]. Chinese Journal of Applied Ecology, 2023, 34(1): 221-228.

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青海地区解磷微生物的筛选及对小油菜生长的影响

Screening of phosphorus solubilizing microorganisms in cold environment and their effects on the growth of Brassica napus

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