桂西北岩溶区不同土地利用方式下土壤反硝化基因丰度及其驱动因素

doi:10.13287/j.1001-9332.202312.013

应用生态学报 ›› 2023, Vol. 34 ›› Issue (12): 3340-3346.doi: 10.13287/j.1001-9332.202312.013

桂西北岩溶区不同土地利用方式下土壤反硝化基因丰度及其驱动因素

万翔宇^1,2, 肖孔操^2,3, 李德军^2,3, 张玉玲¹, 段鹏鹏^2,3*

¹沈阳农业大学土地与环境学院, 沈阳 110866;
²中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室, 长沙 410125;
³中国科学院环江喀斯特生态系统观测研究站, 广西环江 547100

收稿日期:2023-08-30 修回日期:2023-10-23 出版日期:2023-12-15 发布日期:2024-06-15
通讯作者: *E-mail: pengpengduan@isa.ac.cn
作者简介:万翔宇, 男, 1998年生, 硕士研究生。主要从事土壤氮转化过程研究。E-mail: 15144857479@163.com
基金资助:
广西自然科学基金重点项目(2023GXNSFDA026039)、广西自然科学基金项目(2022GXNSFAA035439)和广西优良用材林资源培育重点实验室课题(22-B-01-04)

Abundance of denitrifying genes and their driving factors in soil under different land use types in the karst region of Northwest Guangxi

WAN Xiangyu^1,2, XIAO Kongcao^2,3, LI Dejun^2,3, ZHANG Yuling¹, DUAN Pengpeng^2,3*

¹College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China;
²Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
³Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, Guangxi, China

Received:2023-08-30 Revised:2023-10-23 Online:2023-12-15 Published:2024-06-15

摘要/Abstract

摘要： 为深入了解反硝化微生物在喀斯特区域植被恢复过程中的重要作用,本研究在桂西北采集3种土地利用方式(农田、草地、人工林)的13个土壤样品,测定土壤基本理化性质和反硝化微生物功能基因(nirS、nirK、Fungal nirK、p450nor和nosZ)丰度,研究不同土地利用方式下土壤反硝化微生物功能基因丰度变化及其驱动因素。结果表明: 人工林土壤的pH、有机碳、全氮(TN)、交换性钙(Ca_exe)含量显著高于农田和草地;其土壤nirS、nirK、p450nor和nosZ丰度也显著大于农田和草地。相关性分析显示,pH、TN和Ca_exe与反硝化功能基因nirS、nirK和p450nor丰度呈显著正相关。冗余分析发现,Ca_exe、pH和TN是反硝化功能基因丰度变化的主要影响因素,分别占总解释变量的34.1%、20.1%和16.1%,表明Ca_exe是驱动不同土地利用方式下反硝化功能基因变化的主要因子。综上,桂西北喀斯特区域植被恢复(人工林)能够显著增加土壤反硝化微生物基因丰度,进而影响该生态系统的氮循环过程。

关键词: 土地利用方式, 反硝化细菌, 反硝化真菌, 交换性钙离子

Abstract: To understand the role of denitrifying microbes during vegetation recovery in karst regions, we determined the basic physicochemical properties and abundance of denitrifying microbial functional genes (nirS, nirK, fungal nirK, p450nor, and nosZ) of 13 collected soil samples under three land use types (cropland, grassland, and plantation) in Northwest Guangxi, and investigated the changes in the abundance of denitrifying microbial functional genes and their driving factors. Results showed that soil pH, soil organic carbon, total nitrogen (TN), and exchangeable calcium (Ca_exe) in plantation soil were significantly higher than those in cropland and grassland. The abundance of nirS, nirK, p450nor, and nosZ in plantation soil were significantly higher than those in cropland and grassland. Soil pH, TN, and Ca_exe were positively correlated with the abundance of denitrifying functional genes nirS, nirK, and p450nor. Results of redundancy analysis showed that soil Ca_exe, pH and TN were the primary factors influencing the abundance of denitrifying functional genes, which accounted for 34.1%, 20.1%, and 16.1% of the total variation, respectively. Such a result suggested that Ca_exe was the main driver of changes in denitrifying functional genes under different land use types. Overall, vegetation restoration (plantation) could effectively increase soil denitrifying microbe genes abundance in the karst region of Northwest Guangxi, and consequently influence soil nitrogen cycling.

Key words: land use pattern, denitrifying bacteria, denitrifying fungi, exchangeable calcium

万翔宇, 肖孔操, 李德军, 张玉玲, 段鹏鹏. 桂西北岩溶区不同土地利用方式下土壤反硝化基因丰度及其驱动因素[J]. 应用生态学报, 2023, 34(12): 3340-3346.

WAN Xiangyu, XIAO Kongcao, LI Dejun, ZHANG Yuling, DUAN Pengpeng. Abundance of denitrifying genes and their driving factors in soil under different land use types in the karst region of Northwest Guangxi[J]. Chinese Journal of Applied Ecology, 2023, 34(12): 3340-3346.

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桂西北岩溶区不同土地利用方式下土壤反硝化基因丰度及其驱动因素

Abundance of denitrifying genes and their driving factors in soil under different land use types in the karst region of Northwest Guangxi

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