密旋链霉菌Act12对党参根系形态及药材品质的影响

doi:10.13287/j.1001-9332.202406.014

应用生态学报 ›› 2024, Vol. 35 ›› Issue (6): 1608-1614.doi: 10.13287/j.1001-9332.202406.014

密旋链霉菌Act12对党参根系形态及药材品质的影响

毛仁俊¹, 张国壮², 徐阳¹, 邓醒¹, 阎岩¹, 何志贵^3*, 武文忠⁴

¹延安大学生命科学学院/陕西省红枣重点实验室, 陕西延安 716000;
²中国中医科学院中药研究所, 北京 100700;
³桂林旅游学院食品与健康学院, 广西桂林 541006;
⁴宜君县中医药健康产业发展有限公司, 陕西铜川 727200

收稿日期:2023-12-27 接受日期:2024-04-30 出版日期:2024-06-18 发布日期:2024-12-18
通讯作者: ^*E-mail: James1916@126.com
作者简介:毛仁俊, 男, 1987年生, 博士, 副教授。主要从事药用植物栽培与土壤生态研究。E-mail: mrjnwsuaf@126.com
基金资助:
国家自然科学基金项目(82260748)、陕西省中医药管理局项目(2021-ZZ-ZY004)、宜君野生党参变家种关键技术研究项目(2019-GJ-ZY009)、延安大学产学研合作培育项目(2020-05)和延安大学大学生创新训练项目(D2021123)

Effects of Streptomyces pactum Act12 on root morphogenesis and medicinal quality of Codonopsis pilosula

MAO Renjun¹, ZHANG Guozhuang², XU Yang¹, DENG Xing¹, YAN Yan¹, HE Zhigui^3*, WU Wenzhong⁴

¹College of Life Sciences, Yan’an University/Shaanxi Key Laboratory of Chinese Jujube, Yan’an 716000, Shaanxi, China;
²Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
³College of Food Science and Health, Guilin Tourism University, Guilin 541006, Guangxi, China;
⁴Traditional Chinese Medicine Health Industry Development Co., Ltd. of Yijun County, Tongchuan 727200, Shaanxi, China

Received:2023-12-27 Accepted:2024-04-30 Online:2024-06-18 Published:2024-12-18

摘要/Abstract

摘要： 党参作为一种药食同源的滋补中药,市场需求量很大。本研究以1年生党参种苗为材料开展田间试验,研究复合肥(750 kg·hm^-2)、有机肥(15 t·hm^-2)以及密旋链霉菌Act12菌剂处理(9 t·hm^-2Act12菌剂+10 t·hm^-2有机肥)对党参根系形态、次生代谢物含量以及党参炔苷代谢途径基因表达的影响,旨在明确3种肥料对党参根系形态及药材品质的影响。结果表明: 与对照组(10 t·hm^-2有机肥,传统施肥模式)相比,3种施肥处理均促进了党参根系生长,形成了不同的根系形态;3种施肥处理均提升了党参多糖和次生代谢物含量,其中,Act12菌剂处理显著增加了党参炔苷、苍术内酯Ⅲ和5-羟甲基糠醛的含量。qRT-PCR分析显示,3种施肥处理提升了党参炔苷代谢途径基因的表达水平,其中Act12菌剂处理效果最显著;皮尔逊相关性分析表明,CpHCT和CpFAD基因的表达量与苍术内酯Ⅲ含量呈显著正相关。综上,3种施肥处理均能提升党参的产量与品质,其中施用Act12菌剂处理的效果优于施用复合肥和有机肥处理,是提升党参产量和品质的有效措施。

关键词: Act12菌剂, 党参, 根系形态, 代谢物, 基因表达

Abstract: As a kind of tonic Chinese medicine with dual use in medicine and food, there is a large market demanding for Codonopsis pilosula. Taking one-year-old C. pilosula seedlings as materials, we conducted a field experiment to examine the effect of compound fertilizer (750 kg·hm^-2), organic fertilizer (15 t·hm^-2) and Streptomyces pactum Act12 agent (9 t·hm^-2Act12+10 t·hm^-2 organic fertilizer) treatments on root morphology, secondary metabolite content and expression level of lobetyolin metabolic pathway gene of C. pilosula, to clarify the effects of three fertilizers on the root morphology and medicinal quality. Compared to the control (10 t·hm^-2 organic fertilizer, conventional fertilization), three fertilization treatments could promote root growth and formation. All fertilization treatments promoted the accumulation of C. pilosula polysaccharides and secondary metabolites. Act12 agent significantly increased the content of lobetyolin, atractylenolideIII, and 5-hydroxymethylfurfural. The qRT-PCR analysis indicated that three fertilization treatments increased the expression level of lobetyolin metabolic pathway genes, with Act12 agent treatment showing the most significant effect. Pearson correlation analysis demonstrated that the expression level of CpHCT and CpFAD genes was significantly positively correlated with atractylenolide III content. In conclusion, three fertilization treatments could effectively improve the yield and quality of C. pilosula. Among the three treatments, Act12 agent performed better than that of compound fertilizer and organic fertilizer, which was an effective measure to increase the yield and quality of C. pilosula.

Key words: Act12 agent, Codonopsis pilosula, root morphogenesis, metabolite, gene expression

毛仁俊, 张国壮, 徐阳, 邓醒, 阎岩, 何志贵, 武文忠. 密旋链霉菌Act12对党参根系形态及药材品质的影响[J]. 应用生态学报, 2024, 35(6): 1608-1614.

MAO Renjun, ZHANG Guozhuang, XU Yang, DENG Xing, YAN Yan, HE Zhigui, WU Wenzhong. Effects of Streptomyces pactum Act12 on root morphogenesis and medicinal quality of Codonopsis pilosula[J]. Chinese Journal of Applied Ecology, 2024, 35(6): 1608-1614.

参考文献

[1] 国家药典委员会. 中华人民共和国药典(一部). 北京: 中国医药科技出版社, 2020: 293-294
[2] 兰晓燕, 周利, 李翔, 等. 党参的研究进展及其质量标志物的预测分析. 中国中药杂志, 2023, 48(8): 2020-2040
[3] Gao SM, Liu JS, Wang M, et al. Exploring on the bioactive markers of Codonopsis Radix by correlation analysis between chemical constituents and pharmacological effects. Journal of Ethnopharmacology, 2019, 236: 31-41
[4] Meng Y, Xu YJ, Chang C, et al. Extraction, characterization and anti-inflammatory activities of an inulin-type fructan from Codonopsis pilosula. International Journal of Biology Macromolecules, 2020, 163: 1677-1686
[5] Li JK, Wang Y, Ji JJ, et al. Structural characterization and immunomodulatory activity of a glucan from Radix Codonopsis. Journal of Functional Foods, 2021, 83: 104537
[6] Xu JL, Zhang Z, Zhou K, et al. Integration of network pharmacology and molecular docking technology reveals the mechanism of the herbal pairing of Codonopsis pilosula (Franch.) Nannf and Astragalus membranaceus (Fisch.) Bge on chronic heart failure. Annals of Palliative Medicine, 2021, 10: 7942-7959
[7] 刘杰, 张雄杰, 盛晋华, 等. 肥料类型对盐碱地蒙古黄芪生长与光合特征及药材产量和品质的影响. 西北植物学报, 2021, 41(6): 1012-1018
[8] 李思佳, 邓秋林, 文秋姝, 等. 基于川麦冬活性成分高和重金属含量低的有机无机肥最佳配施方案. 植物营养与肥料学报, 2020, 26(5): 966-974
[9] 钱佳奇, 孙海, 阮音音, 等. 供镁水平对西洋参生长、营养元素吸收分配及品质的影响. 中国中药杂志, 2022, 45(7): 1205-1214
[10] Wambacq E, Alloul A, Grunert O, et al. Aerobes and phototrophs as microbial organic fertilizers: Exploring mineralization, fertilization and plant protection features. PLoS One, 17(2): e0262497
[11] Mulbry W, Westhead EK, Pizarro C, et al. Recycling of manure nutrients: Use of algal biomass from dairy manure treatment as a slow release fertilizer. Bioresource Technology, 2005, 96: 451-458
[12] 刘希港, 李楠, 季托, 等. 微生物菌剂和玉米蛋白酵素对番茄叶片生理特性和产量的影响. 应用生态学报, 2023, 34(11): 3039-3044
[13] 李小玲, 周武先, 蒋小刚, 等. 微生物菌剂对川党参连作障碍及紫纹羽病的防控效果. 中国农业科技导报, 2023, 25(3): 119-131
[14] 张鸿雁, 薛泉宏, 申光辉, 等. 放线菌制剂对人参生长及根域土壤微生物区系的影响. 应用生态学报, 2013, 24(8): 2287-2293
[15] 马军妮, 刘玉涛, 李玉龙, 等. 两株链霉菌对玉米的促生增产作用及机理. 应用生态学报, 2017, 28(1): 315-326
[16] Zhao YS, Sun CY, Wang SZ, et al. Widely targeted metabolomic, transcriptomic, and metagenomic profiling reveal microbe-plant-metabolic reprogramming patterns mediated by Streptomyces pactum Act12 enhance the fruit quality of Capsicum annuum L. Food Research International, 2023, 166: 112587
[17] 阎岩, 赵欣, 张顺仓, 等. 活性氧在密旋链霉菌Act12诱导丹参毛状根中丹参酮积累中的作用. 中国中药杂志, 2014, 39(11): 1985-1991
[18] Yan Y, Zhang SC, Yang DF, et al. Effects of Streptomyces pactum Act12 on Salvia miltiorrhiza hairy root growth and tanshinone synthesis and its mechanisms. Applied Biochemistry and Biotechnology, 2014, 173: 883-893
[19] 胡佳栋, 毛歌, 张志伟, 等. 不同施肥处理对党参产量和次生代谢物含量的影响研究. 中国中药杂志, 2017, 42(15): 2946-2953
[20] 王亚榕, 姜峰, 王晓林, 等. 党参CpPMI基因的克隆与表达分析[EB/OL]. (2022-03-23)[2023-12-27]. 分子植物育种, https://kns.cnki.net/kcms/detail/46.1068.S.20220322.1142.004.html
[21] 李龙, 李超男, 毛新国, 等. 作物根系表型鉴定评价方法的现状与展望. 中国农业科学, 2022, 55(3): 425-437
[22] 周丽平, 袁亮, 赵秉强, 等. 腐殖酸单侧刺激对玉米根系生长的影响. 中国农业科学, 2022, 55(2): 339-349
[23] 曹书苗, 王文科, 王非, 等. 放线菌Act12对Pb胁迫下黑麦草根系生长及抗性生理的影响. 农业环境科学学报, 2016, 35(10): 1881-1887
[24] 汪堃, 南丽丽, 郭全恩, 等. 干旱胁迫对不同根型苜蓿根系构型的影响. 生态学报, 2022, 42(20): 8365-8373
[25] 睿橦, 钟全林, 李宝银, 等. 氮磷配施对刨花楠幼林细根性状的影响. 应用生态学报, 2022, 33(2): 337-343
[26] Mao RJ, Bai ZQ, Wu JW, et al. Transcriptome and HPLC analysis reveal the regulatory mechanisms of aurantio-obtusin in space environment-induced Senna obtusifolia lines. International Journal of Environmental Research and Public Health, 2022, 19: 898
[27] 张甜, 程林, 杨林林, 等. 生态因子及关键酶基因表达对秋季黄芩采收期主要药效成分合成的影响. 中草药, 2019, 50(4): 936-944
[28] 王孟聪, 翁丽丽, 孙金, 等. 肥料配施对北苍术3种倍半萜类成分及2种生物合成关键酶基因表达的影响. 中草药, 2022, 53(13): 4109-4118
[29] 雒阳阳, 张璇, 张福生, 等. 仿野生与平栽蒙古黄芪中黄芪甲苷含量差异的分子机制研究. 中国中药杂志, 2022, 47(13): 3463-3474
[30] 陈士林, 吴问广, 王彩霞, 等. 药用植物分子遗传学研究. 中国中药杂志, 2019, 44(12): 2421-2432
[31] 赵露颖, 施梦瑶, 张巧艳, 等. 道地药材品质特征及形成机制研究进展. 中草药, 2022, 53(21): 6931-6947
[32] 胡佳栋, 武子丁, 刘子哲, 等. 党参氮磷钾施肥效应与最优施肥量研究. 植物营养与肥料学报, 2019, 25(9): 1615-1622
[33] Wang Q, Song J, Zhang JL, et al. Effects of microbial agent application on the bacterial community in ginger rhizosphere soil under different planting years. Frontiers in Microbiology, 2023, 14: 1203796
[34] Li SM, Fan W, Xu G, et al. Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome. Frontiers in Microbiology, 2023, 14: 1117355
[35] Wang NN, Ren J, Wang LL, et al. A preliminary study to explain how Streptomyces pactum (Act12) works on phytoextraction: Soil heavy metal extraction, seed germination, and plant growth. Environmental Monitoring and Assessment, 2023, 195: 757
[36] Wang LL, Wang NN, Guo D, et al. Rhizobacteria helps to explain the enhanced efficiency of phytoextraction strengthened by Streptomyces pactum. Journal of Environmental Sciences, 2023, 125: 73-81

密旋链霉菌Act12对党参根系形态及药材品质的影响

Effects of Streptomyces pactum Act12 on root morphogenesis and medicinal quality of Codonopsis pilosula

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘梅朔, 王传宽, 全先奎. 兴安落叶松叶光合与氮代谢对环境变化响应的转录组分析 [J]. 应用生态学报, 2022, 33(4): 957-962.
[2]	刘深思, 徐贵青, 陈图强, 米晓军, 刘燕, 马健, 李彦. 地下水埋深对幼龄梭梭功能性状的影响 [J]. 应用生态学报, 2022, 33(3): 733-741.
[3]	董晓全, 邢鹤严, 张书源, 陈佳倩, 谢子曦, 邓文琪, 于珊, 吴道铭. 表施和混施污泥对团花根系生长的影响 [J]. 应用生态学报, 2022, 33(12): 3388-3394.
[4]	史帅营, 丁熙柠, 王占超, 郭香凤, 张改娜, 胡永红, 史国安. 不同海拔‘凤丹'牡丹籽粒油脂品质形成及代谢相关基因差异表达 [J]. 应用生态学报, 2022, 33(11): 2987-2996.
[5]	覃潇敏, 潘浩男, 肖靖秀, 汤利, 郑毅. 不同磷水平下玉米-大豆间作系统根系形态变化 [J]. 应用生态学报, 2021, 32(9): 3223-3230.
[6]	柏文恋, 张梦瑶, 刘振洋, 郑毅, 汤利, 肖靖秀. 小麦与蚕豆间作体系根系形态与磷吸收的定量解析 [J]. 应用生态学报, 2021, 32(4): 1317-1326.
[7]	张阔,魏建荣,李臻,杨兵军,王姣雪,苏智. 杨树受机械损伤与光肩星天牛危害的防御性反应 [J]. 应用生态学报, 2021, 32(11): 4139-4146.
[8]	王宇蕴, 李兰, 王瑞雪, 汤利, 郑毅. 不同磷水平下小麦-蚕豆间作根系形态的变化及其与内源激素的关系 [J]. 应用生态学报, 2020, 31(9): 3033-3039.
[9]	吴劲松. 植物对病原微生物的“化学防御”: 植保素的生物合成及其分子调控机制 [J]. 应用生态学报, 2020, 31(7): 2161-2167.
[10]	王金强, 李欢刘庆, 向丹. 干旱胁迫对甘薯苗期根系分化和生理特性的影响 [J]. 应用生态学报, 2019, 30(9): 3155-3163.
[11]	周武先, 何银生, 朱盈徽, 张美德, 段媛媛, 黄大野, 徐大兵, 艾伦强. 生石灰和钙镁磷肥对酸化川党参土壤的改良效果 [J]. 应用生态学报, 2019, 30(9): 3224-3232.
[12]	刘瑞雪, 吴泓瑾, 黄国柱, 赵传燕, 李伟斌. 氮添加对树木根系特性的影响 [J]. 应用生态学报, 2019, 30(5): 1735-1742.
[13]	王红妮, 王学春, 赵长坤, 李军, 秦俭, 龙祖利. 油菜秸秆还田对水稻根系、分蘖和产量的影响 [J]. 应用生态学报, 2019, 30(4): 1243-1252.
[14]	王庆惠, 韩伟, 侯银莹, 冯琳, 叶祖鹏, 顾惠敏, 陈波浪. 不同耐盐品种棉花根系主要指标对盐分胁迫的响应 [J]. 应用生态学报, 2018, 29(3): 865-873.
[15]	郭杰, 刘小平, 张琴, 张东方, 谢彩香, 刘霞. 基于Maxent模型的党参全球潜在分布区预测 [J]. 应用生态学报, 2017, 28(3): 992-1000.