铜对大叶相思-根瘤菌共生固氮体系的影响

摘要/Abstract

摘要： 报道了两种根瘤菌(大叶相思、美丽胡枝子)对Cu²⁺的耐受性以及植物根瘤菌共生固氮体系在Cu²⁺胁迫下结瘤、固氮和生长的变化,讨论了大叶相思在矿山尾矿废弃地作为先锋植物结瘤固氮的可能性.结果表明,大叶相思根瘤菌对Cu²⁺离子的耐受性较强,可以耐受Cu²⁺<080mmol的离子浓度,Cu²⁺对它的半致死浓度为0.129mmol.在无菌砂培无重金属影响条件下,其固氮酶活性为27C₂H₄·μg·g^-1·h^-1,当Cu²⁺>0.125mmol会导致大叶相思固氮酶活性急剧下降,其有效半抑制浓度(EC₅₀)为0.151mmol,Cu²⁺为0.50mmol完色抑制大叶相思固氮酶活性,不阻碍结瘤,但严重抑制植物生长发育,引起植物叶片白化、植株矮化.在外加N源不接菌和不加N源接菌两种处理组间,Cu²⁺<0.125mmol时,以不加N接菌处理对大叶相思生长有利.大叶相思对Cu²⁺吸收积累根部高于地上组织.

关键词: 铜, 根瘤菌, 大叶相思, 生长, 结瘤, 固氮酶活性, 杉木, 生物量分配, 幼苗出土, 形态可塑性, 遮阴, 天然更新

Abstract: The effects of different levels of Cu²⁺ were studied individually on the growth of two Rhizobium strains and host plant growth,nodulation(%) and nitrogen fixation activity in A.auriculaeformis under solution culture conditions so as to explore a probability of utilization of the genus Acacia in revegetation of Cu²⁺-contaminated land.The results shows that Rhizobium sp.(Acacia auriculaeformis)had more stronger tolerance to Cu²⁺ iron than Rhizobium sp(Lespedeza formosa).The values of EC₅₀ respectively achieved 0.129mmol for Rhizobium sp.and 0.151mmol for the N₂-fixation activity.When the concentration of Cu²⁺ was over 0.50 mmol,N₂-fixation activity had been inhabited thoroughly.Although the formation of nodules of Acacia auriculaeformis could continued,the growth of host plant had been inhibited with the appearance such as stunting,chlorosis. Between the noinodulating but adding nitrogen treat group and the inodulating without any nitrogen resource treat group, when Cu²⁺<0.125mmol, the latter group showed better growth for A. auriculaeformis. There were higher contents of Cu in the underground tissues than in aboveground tissues.

Key words: Cu, Rhizobium sp, Acacia auriculaeformis, Growth, Formation of nodules, N₂-fixation activity, seedling emergence, morphological plasticity, shade, Chinese fir, biomass allocation, natural regeneration

中图分类号:

X503.235

聂湘平, 蓝崇钰, 张志权, 束文圣, 黄铭洪. 铜对大叶相思-根瘤菌共生固氮体系的影响[J]. 应用生态学报, 2002, (2): 137-140.

NIE Xiangping, LAN Chongyu, ZHANG Zhiquan, SHU Wensheng, HUANG Minghong . Effects of copper on rhizobia-Acacia auriculaeformis symbiotic association[J]. Chinese Journal of Applied Ecology, 2002, (2): 137-140.

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