关键词: 斜纹夜蛾, 核多角体病毒, 疾病流行学, 时间-剂量-死亡率模型

Abstract: In this paper,the effects of transgenes insertion on the outcross potentiality of rice varieties were assessed by pollen vigor and hybrid seed set.The in vitro pollen germination rates of five transgenic rice lines transformed respectively with bar,crylAb,BADH and Xa21 gene were investigated,and compared with their relative receptor rice varieties.The results showed that there existed a significant difference in in vitro pollen germination rates between receptor rice varieties,but no significant difference was found between transgenic rice varieties and their relative receptors.The in vitro pollen germination rate for transgenic rice varieties varied from 0.416 to 0.584,similar to that of their relative receptors(0.400～0.574). Investigation on the hybrid seed set of 26 hand-crosses showed that the inserted bar or crylAb gene had a significant effect on the hybrid seed set of receptors,while the effect of Xa21 gene was smaller.The hybrid seed set rate of nontransgenic rice in crosses with transgenic rice(pollen donor)was from 0.056 to 0.413,not different from that in crosses with their relative receptors(0.052～0.417).It's suggested that transgenes insertion had little effect on the outcross potentiality of rice varieties.

Key words: Spodoptera litura, Nuclear polyhedrosis virus, Disease prevalence, Time-dosage-mortality model