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葡萄糖存在下选择性抑菌剂对土壤氮素转化的影响

王鸽1,2;何红波2;张旭东2;李建东1;韩琳1,2;汪景宽1   

  1. 1沈阳农业大学,沈阳 110161;2中国科学院沈阳应用生态研究所陆地生态过程重点实验室,沈阳 110016
  • 收稿日期:2005-10-08 修回日期:2006-02-13 出版日期:2006-05-18 发布日期:2006-05-18

Effects of specific microbial biocides on N transformation in soil with glucose amendment

WANG Ge1,2;HE Hongbo2;ZHANG Xudong2;LI Jiandong1;HAN Lin1,2;WANG Jingkuan1   

  1. 1Shenyang Agricultural University,Shenyang 110161,China; 2Key Laboratory of Terrestrial Ecological Process,Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China
  • Received:2005-10-08 Revised:2006-02-13 Online:2006-05-18 Published:2006-05-18

摘要: 采用2种氮源并分别加入选择性微生物抑制剂进行室内培养,通过测定样品中NH4+-N和NO3--N及土壤中氨基葡萄糖和胞壁酸含量,研究土壤微生物氮素固持时间特征及其相对贡献.结果表明,加入青链霉素明显地降低了NH4+-N的转化速率,且影响远大于真菌抑制剂放线菌酮;氨基葡萄糖和胞壁酸的相对比例急剧增加,而后趋于平衡;加入放线菌酮后NO3-N转化速率持续下降,氨基葡萄糖的合成受到抑制,但加入细菌抑制剂青链霉素对其转化无显著影响.培养初期,细菌在葡萄糖存在下能快速固持NH4+-N和NO3--N,并以NH4+-N为首选氮源;培养后期,氮转化主要为真菌所推动,且真菌对NO3--N的利用能力显著大于细菌.

关键词: 褐飞虱, 热休克反应, 保护酶系, 脂质过氧化物

Abstract: In an incubation test of soil with glucose amendment,two kinds of nitrogenous fertilizer and three kinds of specific microbial biocides were applied,and the contents of soil NH4+-N,NO3--N,glucosamine and muramic acid were measured to differentiate the relative contribution and timing characteristics of soil microbes in nitrogen immobilization.The results showed that penicillin and streptomycin decreased the transformation rate of NH4+-N markedly,with more significant effects than actidione.The amount ratio of glucosamine to muramic acid after applying penicillin and streptomycin rapidly increased first,and tended to equilibrium then.With the application of actidione,the transformation rate of NO3--N decreased continuously,and the synthesis of glucosamine was inhibited,while penicillin and streptomycin had no significant effects on them.At the early stage of incubation,bacteria could rapidly immobilize both NH4+-N and ,NO3--N with NH4+-N preferred,while at the later stage of incubation,fungi were the dominant contributor to nitrogen transformation,and had much stronger ability of utilizing NO3--N than bacteria.

Key words: Nilaparvata lugens, Heat shock response, Endogenous enzymes of protective system, Lipid peroxides