脂类抽提对北太平洋柔鱼肌肉碳、氮稳定同位素测定结果的影响

应用生态学报

脂类抽提对北太平洋柔鱼肌肉碳、氮稳定同位素测定结果的影响

贡艺¹,陈新军^1,2，3,高春霞^1,2，3,李云凯^1,2，3**

(¹上海海洋大学海洋科学学院，上海 201306; ²国家远洋渔业工程技术中心，上海 201306； ^3大洋渔业资源可持续开发省部共建教育部重点实验室，上海 201306)

出版日期:2014-11-18 发布日期:2014-11-18

Effects of lipid extraction on stable carbon and nitrogen isotope analyses of Ommastrephes bartramii muscle

GONG Yi¹, CHEN Xin-jun^1,2，3, Gao Chun-xia^1,2，3, LI Yun-kai^1,2，3

(¹College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China; ^2National Engineering Research Centre for Oceanic Fisheries, Shanghai 201306, China; ³Ministry of Education Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Shanghai 201306, China)

Online:2014-11-18 Published:2014-11-18

摘要/Abstract

摘要： 稳定同位素技术可深层次地分析头足类的摄食习性和栖息地等方面的重要信息.稳定同位素分析多选取动物肌肉为研究组织，然而，肌肉中的脂类含量会影响对其稳定同位素信息的准确解析.北太平洋柔鱼是重要的大洋经济性头足类，在海洋生态系统中具有重要的生态地位.本研究选取了53尾北太平洋柔鱼，通过分析脂类抽提对胴体肌肉稳定同位素比值的影响，探讨脂类物质对稳定同位素比值的干扰机制，对比不同碳稳定同位素比值校正模型的适用性，并提出适用于北太平洋柔鱼胴体肌肉δ¹³-C校正模型.结果表明：北太平洋柔鱼肌肉的脂类抽提会引起稳定同位素测定结果的显著变化，δ¹³-C和δ¹⁵-N分别平均升高0.71‰和0.47‰，在分析组织碳稳定同位素比值时，脂类抽提在样品预处理过程中十分必要，其能消除脂类对样品稳定同位素分析的干扰，从而更加准确地分析研究对象食性与栖息地的变化.在单独进行氮稳定同位素比值分析时，则不需要进行脂类抽提.

Abstract:

Stable isotope analysis (SIA) has become an important tool to investigate diet shift, habitat use and trophic structure of animal population. Muscle is considered to be the most common tissue for SIA, however, lipid content in muscle causes a considerable bias to the interpretation of isotopic ratios of animals. Neon flying squid (Ommastrephes bartramii) is an important economic cephalopod of Chinese distant water fishery, and plays a major role in marine ecosystems. In this study, the effects of lipid extraction on stable isotope ratios of the muscles of 53 neon flying squids were investigated and the interference mechanism of lipid in SIA was clarified with the aim of contrasting the suitability of different lipid correction models of stable carbon isotope. Results showed that the stable carbon and nitrogen isotopic values of non-lipid extracted samples significantly increased after lipid extractions by 0.71‰ and 0.47‰, respectively, which suggested that lipid extraction in cephalopod isotope study is needed prior to stable carbon isotope analysis but not recommended for stable nitrogen isotope analysis. The results could help remove the effects of lipid contents and standardize SIA muscle samples, thereby getting better understanding of the isotopic change of neon flying squids in the future.

贡艺1,陈新军1,2，3,高春霞1,2，3,李云凯1,2，3**. 脂类抽提对北太平洋柔鱼肌肉碳、氮稳定同位素测定结果的影响[J]. 应用生态学报.

GONG Yi1, CHEN Xin-jun1,2，3, Gao Chun-xia1,2，3, LI Yun-kai1,2，3. Effects of lipid extraction on stable carbon and nitrogen isotope analyses of Ommastrephes bartramii muscle[J]. Chinese Journal of Applied Ecology.