大兴安岭棕色针叶林土壤的真菌多样性

doi:10.13287/j.1001-9332.201910.030

应用生态学报 ›› 2019, Vol. 30 ›› Issue (10): 3411-3418.doi: 10.13287/j.1001-9332.201910.030

大兴安岭棕色针叶林土壤的真菌多样性

杨立宾^1,2,3, 隋心³, 魏丹¹, 崔福星¹, 朱道光¹, 倪红伟^1*

¹黑龙江省科学院自然与生态研究所, 哈尔滨 150040;
²黑龙江省科学院高技术研究院, 哈尔滨 150020;
³黑龙江大学生命科学学院黑龙江省寒地生态修复与资源利用重点实验室, 哈尔滨 150080

收稿日期:2019-03-19 出版日期:2019-10-20 发布日期:2019-10-20
通讯作者: *E-mail: nihongwei2000@163.com
作者简介:杨立宾, 男, 1981年生, 博士, 副研究员, 硕士研究生导师. 主要从事微生物生态学研究. E-mail: ylb1128@qq.com
基金资助:
国家自然科学基金项目(31600396)和黑龙江省科学院科研专项(YY2018ZR02,XKLY2019ZR01,ZNBZ2017ZR02,CXMS2018ZR03)资助

Fungal diversity in the brown coniferous forest soils of Daxing’anling Mountains, Northeast China

YANG Li-bin^1,2,3, SUI Xin³, WEI Dan¹, CUI Fu-xing¹, ZHU Dao-guang¹, NI Hong-wei^1*

¹Institute of Nature & Ecology, Heilongjiang Academy of Sciences, Harbin 150040, China;
² Institute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin 150020, China;
³Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin 150080, China

Received:2019-03-19 Online:2019-10-20 Published:2019-10-20
Contact: *E-mail: nihongwei2000@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31600396) and the Special Scientific Research Program of Heilongjiang Academy of Sciences (YY2018ZR02, XKLY2019ZR01, ZNBZ2017ZR02,CXMS2018ZR03).

摘要/Abstract

摘要： 为探究大兴安岭棕色针叶林土壤的真菌群落分布特征及其与土壤环境因子间的关系,采用高通量测序技术对大兴安岭呼中自然保护区兴安落叶松林的表浅棕色针叶林土、生草棕色针叶林土和典型棕色针叶林土的土壤真菌群落结构和多样性进行分析,运用R语言、SPSS 17.0等软件对土壤真菌群落与土壤环境因子间进行冗余分析和相关性分析.结果表明: 3个亚类的棕色针叶林土壤的理化性质间存在显著性差异.测序共获得6门23纲46目74科88属111种的土壤真菌,其中,子囊菌门、担子菌门为优势菌门;门分类水平上各样地菌群的相对丰度存在显著性差异.土壤真菌Ace和Chao1多样性指数在各亚类土壤间无显著性差异, 浅棕色针叶林土壤样地的Shannon和Simpson指数与其他样地存在显著差异.典型关联分析和相关性分析发现,土壤真菌的α和β多样性与土壤含水率、pH值、有机质、全氮、全钾等显著相关.说明土壤环境因子是影响大兴安岭兴安落叶松棕色针叶林土壤真菌多样性的重要因素.

Abstract: To explore the distribution of fungal community in soil of brown coniferous forest in Daxing’anling and its relationship with soil characteristics, soil fungal community structure and diversity in three forest types, light brown coniferous forest, grass brown coniferous forest, and typical brown coniferous forest, were analyzed by high-throughput sequencing technology. The relationship between soil fungal community and soil characteristics was analyzed by R and SPSS 17.0 software. The results showed that soil physical and chemical characteristics differed significantly across the three brown coniferous forests. A total of 111 species belonged to six phyla, 23 class, 46 orders, 74 families and 88 genera were recorded in this study. Ascomycetes and basidiomycetes were dominant phyla. There were significant differences in relative abundance of phyla among three forest types. There was no significant difference in the Ace and Chao1 diversity indices among three brown coniferous forests, whereas there were significant differences in the Shannon and Simpson indices. Results from canonical correlation analysis and correlation analysis showed that both α and β diversity were significantly correlated with soil moisture, soil pH, soil organic matter, soil total nitrogen, and soil total potassium. The results indicated that soil characteristics were important factors affecting soil fungal diversity of brown coniferous forest in Xing’an larch forest in Daxing’anling.

杨立宾, 隋心, 魏丹, 崔福星, 朱道光, 倪红伟. 大兴安岭棕色针叶林土壤的真菌多样性[J]. 应用生态学报, 2019, 30(10): 3411-3418.

YANG Li-bin, SUI Xin, WEI Dan, CUI Fu-xing, ZHU Dao-guang, NI Hong-wei. Fungal diversity in the brown coniferous forest soils of Daxing’anling Mountains, Northeast China[J]. Chinese Journal of Applied Ecology, 2019, 30(10): 3411-3418.

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大兴安岭棕色针叶林土壤的真菌多样性

Fungal diversity in the brown coniferous forest soils of Daxing’anling Mountains, Northeast China

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