农抗702对植物病原真菌的抑制效果及抑菌机理

应用生态学报

农抗702对植物病原真菌的抑制效果及抑菌机理

魏赛金^1,2,杜亚楠¹,倪国荣²,张慧雯¹,涂国全¹,潘晓华^2**

(¹江西农业大学生物科学与工程学院，南昌 330045； ²江西农业大学作物生理生态与遗传育种教育部重点实验室/江西省作物生理生态与遗传育种重点实验室，南昌 330045)

出版日期:2012-12-18 发布日期:2012-12-18

Inhibition effect of Ag-antibiotic 702 on plant pathogenic fungi and related mechanisms.

WEI Sai-jin^1,2, DU Ya-nan¹, NI Guo-rong², ZHANG Hui-wen¹, TU Guo-quan¹, PAN Xiao-hua²

(¹College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China; ²Ministry of Education Key Laboratory of Crop Physiology, Ecology and Genetic Breeding / Jiangxi Province Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, JAU, Nanchang 330045, China)

Online:2012-12-18 Published:2012-12-18

摘要/Abstract

摘要： 为探索农抗702在植物病害生物防治中的实际应用价值及对病原真菌的作用机理，采用生长速率法测定其抑菌谱，并以水稻纹枯病菌为研究对象，测定其作用下菌丝的电解质渗漏、蛋白质、核酸、Mg²⁺、K⁺含量的变化，及其对细胞膜麦角甾醇生物合成和水稻纹枯病菌超显微结构的影响.结果表明: 农抗702对供试13种病原真菌均有较强的抑制作用，其中对油菜菌核病菌的作用最强，EC₅₀为0.23 μg·mL^-1；农抗702作用水稻纹枯病菌菌体后相对电导率增加72.2%；蛋白质、核酸、Mg²⁺、K⁺等胞内物质均发生了不同程度的渗漏；细胞麦角甾醇含量降低了92.0%；细胞膜轮廓不清、破损；细胞器严重损伤，有空泡化.抑制麦角甾醇合成、造成真菌细胞膜渗透增加是农抗702抑制真菌的主要机理.

Abstract: To explore the practical application value and action mechanisms of Ag-antibiotic 702 against pathogenic fungi, the inhibition spectrum of Ag-antibiotic 702 was studied by measuring the mycelium growth rate of pathogenic fungi, and the effects of Ag-antibiotic 702 on the membrane permeability of Rhizoctonia solani, a typical pathogenic fungus, were investigated, with the variations of mycelium electrolyte leakage and protein, nucleic acid, and Mg²⁺ and K⁺ contents under the action of Ag-antibiotic 702 determined, and the effects of Ag-antibiotic 702 on the cell membrane ergosterol biosynthesis and ultramicrostructure observed. The results showed that the active products of Ag-antibiotic 702 had stronger inhibition effect on 13 test pathogens, among which, Sclerotinia sclerotiorum was most sensitive, with the EC₅₀ being 0.23 μg·mL^-1. As compared with the control, the relative electric conductivity of R. solani treated with Ag-antibiotic 702 was increased by 72.2%, the contents of protein, nucleic acid, and Mg²⁺ and K⁺ leaked from the R. solani cells were all increased, while the ergosterol content was decreased by 92.0%. The cell membrane outline was not clear, organelles were badly damaged, and vacuole appeared. It was suggested that the inhibition of ergosterol biosynthesis and the increase of membrane permeability could be the main action mechanisms of Ag-antibiotic 702 against pathogenic fungi.

魏赛金1,2,杜亚楠1,倪国荣2,张慧雯1,涂国全1,潘晓华2**. 农抗702对植物病原真菌的抑制效果及抑菌机理[J]. 应用生态学报.

WEI Sai-jin1,2, DU Ya-nan1, NI Guo-rong2, ZHANG Hui-wen1, TU Guo-quan1, PAN Xiao-hua2. Inhibition effect of Ag-antibiotic 702 on plant pathogenic fungi and related mechanisms.[J]. Chinese Journal of Applied Ecology.