甜瓜根系分泌物中酚酸物质对尖孢镰孢菌的化感效应

应用生态学报

甜瓜根系分泌物中酚酸物质对尖孢镰孢菌的化感效应

杨瑞秀¹,高增贵^1**,姚远¹,刘限²,孙淑清²,王莹³

(¹College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China; ²College of Biosciences and Technology, Shenyang Agricultural University, Shenyang 110866, China; ³Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China)

出版日期:2014-08-18 发布日期:2014-08-18

Allelopathic effects of phenolic compounds of melon root exudates on Fusarium oxysporum f. sp. melonis

(¹沈阳农业大学植物保护学院, 沈阳 110866; ^2沈阳农业大学生物科学技术学院, 沈阳 110866; ³中国科学院沈阳应用生态研究所, 沈阳 110016)

Online:2014-08-18 Published:2014-08-18

摘要/Abstract

摘要：

采用HPLC法对甜瓜根系分泌物进行分离鉴定,检测到甜瓜根系分泌物中含有没食子酸、邻苯二甲酸、丁香酸、水杨酸、阿魏酸、苯甲酸和肉桂酸7种酚酸物质,通过外源添加法研究该类物质对尖孢镰孢菌的化感效应.室内试验结果表明: 阿魏酸、苯甲酸、肉桂酸在0.1、0.25 mmol·L^-1处理浓度下能够显著促进尖孢镰孢菌的孢子萌发,水杨酸则对孢子萌发具有一定的抑制作用；丁香酸、阿魏酸在菌丝培养后期表现出较强的促进作用.盆栽结果显示,在0.05、0.1和0.5 mmol·L^-1处理浓度下肉桂酸、阿魏酸、苯甲酸可显著促进甜瓜枯萎病病情.

Abstract:

In this study, the phenolic compounds of melon root exudates were identified by HPLC and seven phenolic compounds including gallic acid, phthalic acid, syringic acid, salicylic acid, ferulic acid, benzoic acid and cinnamic acid were observed. The laboratory experiment showed that ferulic acid, benzoic acid and cinnamic acid of 0.1 and 0.25 mmol·L^-1 could significantly promote the germination of Fusarium oxysporum f. sp. melonis spore while salicylic acid inhibited the spore germination to some degree. Syringic acid and ferulic acid significantly promoted the mycelium growth at the late stage of incubation. The pot experiments demonstrated that cinnamic acid, benzoic acid and ferulic acid enhanced melon infection at concentrations of 0.05, 0.1 and 0.5 mmol·L^-1.

杨瑞秀1,高增贵1**,姚远1,刘限2,孙淑清2,王莹3. 甜瓜根系分泌物中酚酸物质对尖孢镰孢菌的化感效应[J]. 应用生态学报.