鳜塘浮游生物DNA序列多样性、水质和疾病的关系

应用生态学报

鳜塘浮游生物DNA序列多样性、水质和疾病的关系

王亚军^1，2;吴淑勤¹;林文辉¹;杨智慧¹;吴会民¹;石存斌¹;潘厚军¹

¹中国水产科学研究院珠江水产研究所，广州 510380；
²上海水产大学生命科学与技术学院，上海 200090

收稿日期:2005-12-30 修回日期:2006-10-31 出版日期:2007-01-18 发布日期:2007-01-18

Relationships among planktons DNA sequence diversity, water quality and fish diseases in Siniperca chuatsi ponds

WANG Ya-jun^1,2;WU Shu-qin¹;LIN Wen-hui¹;YANG Zhi-hui¹;WU Hui-min¹;SHI Cun-bin¹;PAN Hou-jun¹

¹Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380,
China;
²College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 20090,
China

Received:2005-12-30 Revised:2006-10-31 Online:2007-01-18 Published:2007-01-18

摘要/Abstract

摘要： 应用RAPD技术研究了鳜塘水生生态系统中浮游生物群落α-多样性，分析了浮游生物群落DNA序列丰富度与水质和疾病发生之间的关系，探讨了池塘养殖密度和短周期、小密度、高投饵量养殖方式对浮游生物群落DNA序列丰富度及水质的影响.结果表明：1)鳜塘浮游生物群落DNA序列丰富度与水质综合指数呈显著负相关关系(P<0.01)； 2)池塘高密度养殖会造成浮游生物丰富度降低和水质综合指数升高；3)短周期、小密度、高投饵量的养殖模式对环境的损伤较大；4)浮游生物群落DNA指纹01矩阵和水质理化因子样品聚类分析表明，发病塘在水质理化因子和浮游生物群落上具有相似性，为鳜疾病预报模型的建立奠定了基础.

关键词: 太湖地区, 稻季, 养分淋洗

Abstract: By using random amplified polymorphic DNA (RAPD) technique, this paper studied the α-diversity of plankton communities and its relationships with water quality and fish diseases in 7 Siniperca chuatsi ponds, as well as the effects of stocking density and a new culture model on the diversity and water quality. The results showed that there was a significant negative correlation between the DNA sequence diversity of plankton communities and water quality index, and high stocking density decreased the DNA sequence diversity and increased the water quality index. The new culture model with short culture period, low stocking density and high feeding stuff input had a greater damage on the water environment. Hierarchical cluster analysis indicated that there existed similarities in the DNA sequences of plankton communities and the physicochemical properties of water bodies in the ponds with fish diseases, which provided a possibility to predict the diseases occurrence in Siniperca chuatsi ponds.

Key words: Nutrient leaching, Paddy field, Taihu Lake area

王亚军^1，2;吴淑勤¹;林文辉¹;杨智慧¹;吴会民¹;石存斌¹;潘厚军¹. 鳜塘浮游生物DNA序列多样性、水质和疾病的关系[J]. 应用生态学报.

WANG Ya-jun^1,2;WU Shu-qin¹;LIN Wen-hui¹;YANG Zhi-hui¹;WU Hui-min¹;SHI Cun-bin¹;PAN Hou-jun¹ . Relationships among planktons DNA sequence diversity, water quality and fish diseases in Siniperca chuatsi ponds [J]. Chinese Journal of Applied Ecology.

[1]	胡安永1,2,孙星1,刘勤1*. 太湖地区不同轮作模式对稻田温室气体(CH₄和N₂O)排放的影响 [J]. 应用生态学报, 2016, 27(1): 99-106.
[2]	赵冬1,2,3,颜廷梅1**,乔俊1,3,杨林章1,汤芳4,宋云飞4. 太湖地区绿肥还田模式下氮肥的深度减量效应 [J]. 应用生态学报, 2015, 26(6): 1673-1678.
[3]	王小治,张海进,孙伟,封克,朱建国. 大气CO₂浓度升高对稻田土壤氮素的影响 [J]. 应用生态学报, 2010, 21(08): 2161-2165.
[4]	王小治;尹微琴;单玉华;封克;朱建国. 太湖地区湿沉降中氮磷输入量 ——以常熟生态站为例 [J]. 应用生态学报, 2009, 20(10): 2487-2492.
[5]	王小治, 朱建国, 高人, 宝川靖和. 太湖地区氮素湿沉降动态及生态学意义:以常熟生态站为例 [J]. 应用生态学报, 2004, (9): 1616-1620.
[6]	王小治, 朱建国, 高人, 宝川靖和. 太湖地区氮素湿沉降动态及生态学意义:以常熟生态站为例 [J]. 应用生态学报, 2004, (9): 1616-1620.
[7]	王小治[1] 朱建国[1] 高人[2] 宝川靖和[3]. 太湖地区氮素湿沉降动态及生态学意义：以常熟生态站为例 [J]. 应用生态学报, 2004, 15(09): 1616-1620 .
[8]	连纲, 王德建, 林静慧, 龑德智. 太湖地区稻田土壤养分淋洗特征 [J]. 应用生态学报, 2003, (11): 1879-1883.
[9]	连纲, 王德建, 林静慧, 龑德智. 太湖地区稻田土壤养分淋洗特征 [J]. 应用生态学报, 2003, (11): 1879-1883.
[10]	王校常, 曹志洪. 太湖地区水稻土的健康质量问题及调控对策 [J]. 应用生态学报, 2002, (2): 199-203.
[11]	潘根兴, 韩永镜, 诸清河. 太湖地区土壤环境中SAS的分布和降解特点 [J]. 应用生态学报, 2002, (2): 171-174.