关键词: 水平基因转移, 三亲配对外源质粒分离, 广宿主质粒, 石油烃降解, 生物修复

Abstract: The correlations of rice plant nitrogen content with raw hyperspectral reflectance, first derivative hyperspectral reflectance, and hyperspectral characteristic parameters were analyzed, and the hyperspectral remote sensing diagnosis models of rice plant nitrogen nutritional status with these remote sensing parameters as independent variables were constructed and validated. The results indicated that the nitrogen content in rice plant organs had a variation trend of stem<sheath<spike<leaf. The spectral reflectance at visible light bands was leaf<spike<sheath<stem, but that at nearinfrared bands was in adverse. The linear and exponential models with the raw hyperspectral reflectance at 796.7 nm and the first derivative hyperspectral reflectance at 738.4 nm as independent variables could better diagnose rice plant nitrogen nutritional status, with the decisive coefficients (R²) being 0.7996 and 0.606, respectively; while the model with vegetation index (SD_r-SD_b)/(SD_r+SD_b) as independent variable, i.e., y=365.871+639.323((SD_r-SD_b)/(SD_r+SD_b)), was most fit rice plant nitrogen content, with R²=0.8755, RMSE=0.2372 and relative error=11.36%, being able to quantitatively diagnose the nitrogen nutritional status of rice.

Key words: horizontal gene transfer, exogenous plasmid isolation by triparental mating, broad host range plasmid, degradation of petroleum hydrocarbon, bioremediation.