木霉对盐渍逆境下枸杞根系氮素吸收和氮素利用效率的影响

doi:10.13287/j.1001-9332.202209.015

应用生态学报 ›› 2022, Vol. 33 ›› Issue (9): 2539-2546.doi: 10.13287/j.1001-9332.202209.015

木霉对盐渍逆境下枸杞根系氮素吸收和氮素利用效率的影响

梅惠敏¹, 阮亚男¹, 张家欣¹, 崔金鑫², 颜坤^2*, 董小燕³, 边兰星⁴, 孙艳虹⁵

¹辽宁大学生命科学院, 沈阳 110000;
²鲁东大学农学院, 山东烟台 264025;
³中国科学院烟台海岸带研究所, 中国科学院海岸带环境过程与生态修复重点实验室, 山东烟台 264003;
⁴烟台大学生命科学学院, 山东烟台 264005;
⁵烟台大学环境与材料工程学院, 山东烟台 264005

收稿日期:2021-12-06 接受日期:2022-07-13 出版日期:2022-09-15 发布日期:2023-03-15
通讯作者: * E-mail: kyan@ldu.edu.cn; yankunacademic@163.com
作者简介:梅惠敏, 女, 1996年生, 硕士研究生。主要从事植物生理生态学研究。E-mail: 924916779@qq.com
基金资助:
国家自然科学基金-山东联合基金项目(U2106214)、国家重点研发计划项目(2019YFD1002702)和烟台市科技创新发展计划项目(2020MSGY065)资助。

Effects of Trichoderma on nitrogen absorption and use efficiency in Lycium chinense roots under saline stress

MEI Hui-min¹, RUAN Ya-nan¹, ZHANG Jia-xin¹, CUI Jin-xin², YAN Kun²^*, DONG Xiao-yan³, BIAN Lan-xing⁴, SUN Yan-hong⁵

¹College of Life Science, Liaoning University, Shenyang 110000, China;
²School of Agriculture, Ludong University, Yantai 264025, Shandong, China;
³CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong, China;
⁴College of Life Science, Yantai University, Yantai 264005, Shandong, China;
⁵College of Environmental and Material Engineering, Yantai University, Yantai 264005, Shandong, China

Received:2021-12-06 Accepted:2022-07-13 Online:2022-09-15 Published:2023-03-15

摘要/Abstract

摘要： 本研究采集滨海盐渍土开展盆栽试验,分析施加有机肥、木霉菌剂及菌肥对枸杞氮素吸收、同化、积累和利用效率的影响,以揭示木霉对盐渍逆境下枸杞的促生机理。有机肥为木霉菌肥的灭菌物,不含木霉活菌,但两者氮、磷、钾等养分含量无显著差异。结果表明: 施加有机肥、木霉菌剂和菌肥处理较对照均显著提高了根系分生区NO₃^-、NH₄⁺内流速率和成熟区NO₃^-内流速率,且施加菌肥的提升幅度高于施加有机肥。与对照相比,盐渍土壤施加木霉菌剂及菌肥显著增加了根、茎、叶生物量和氮含量以及植株氮累积量,增强了枸杞根和叶中硝酸还原酶、亚硝酸还原酶和谷氨酰胺合成酶活性,提高了枸杞氮素吸收效率、光合速率、稳定碳同位素丰度值和光合氮素利用效率,而且施加菌肥的效果明显优于施加有机肥。综上,木霉能增强盐渍逆境下枸杞氮素吸收、同化和积累,提升光合固碳能力和氮素利用效率,最终促进植株生长。

关键词: 滨海盐渍土, 枸杞, 光合固碳, 氮离子流速

Abstract: To clarify the mechanisms underlying the improvement of Trichoderma on Chinese wolfberry (Lycium chinense) growth under saline stress, we analyzed the effects of application of organic fertilizer, Trichoderma agent and fertilizer on nitrogen uptake, assimilation, accumulation and use efficiency in Chinese wolfberry, based on a pot experiment with coastal saline soil. The organic fertilizer was the sterilization substance of Trichoderma fertilizer without viable Trichoderma, without any difference in the content of nutrients (such as nitrogen, phosphorus and potassium) between them. The results showed that the application of organic fertilizer, Trichoderma agent and ferti-lizer significantly increased NO₃^- and NH₄⁺ influx rate in meristematic zone and NO₃^- influx rate in maturation zone of roots. The magnitude of such enhancement was greater in the application with Trichoderma fertilizer than organic fertilizer. Compared with the control, the application of Trichoderma agent and fertilizer significantly increased root, stem and leaf biomass and nitrogen content as well as plant nitrogen accumulation, strengthened root and leaf nitrate reductase, nitrite reductase and glutamine synthetase activities, and elevated nitrogen uptake efficiency, photosynthetic rate, stable carbon isotope abundance and photosynthetic nitrogen use efficiency. For all those variables, the beneficial effect was obviously stronger in the application with Trichoderma fertilizer than organic fertilizer. Therefore, Trichoderma facilitated nitrogen uptake, assimilation and accumulation in Chinese wolfberry under saline stress, improved photosynthetic carbon fixation ability and nitrogen use efficiency, and ultimately promoted plant growth.

Key words: coastal saline soil, Chinese wolfberry, photosynthetic carbon fixation, nitrogen ion flux

梅惠敏, 阮亚男, 张家欣, 崔金鑫, 颜坤, 董小燕, 边兰星, 孙艳虹. 木霉对盐渍逆境下枸杞根系氮素吸收和氮素利用效率的影响[J]. 应用生态学报, 2022, 33(9): 2539-2546.

MEI Hui-min, RUAN Ya-nan, ZHANG Jia-xin, CUI Jin-xin, YAN Kun, DONG Xiao-yan, BIAN Lan-xing, SUN Yan-hong. Effects of Trichoderma on nitrogen absorption and use efficiency in Lycium chinense roots under saline stress[J]. Chinese Journal of Applied Ecology, 2022, 33(9): 2539-2546.

参考文献

[1] Long XH, Liu LP, Shao TY, et al. Developing and sustainably utilize the coastal mudflat areas in China. Science of the Total Environment, 2016, 569: 1077-1086
[2] 吕晓, 徐慧, 李丽, 等. 盐碱地农业可持续利用及其评价. 土壤, 2012, 44(2): 203-207
[3] 孔涛, 张德胜, 徐慧,等. 盐碱地及其改良过程中土壤微生物生态特征研究进展. 土壤, 2014, 46(4): 581-588
[4] 刘涛, 鲁剑巍, 任涛, 等. 不同氮水平下冬油菜光合氮利用效率与光合器官氮分配的关系. 植物营养与肥料学报, 2016, 22(2): 518-524
[5] 辛正琦, 代欢欢, 辛余凤, 等. 盐胁迫下外源2,4-表油菜素内酯对颠茄氮代谢及TAs代谢的影响. 作物学报, 2021, 47(10): 2001-2011
[6] Soussi M, Lluch C, Ocaña A, et al. Comparative study of nitrogen fixation and carbon metabolism in two chick-pea (Cicer arietinum L.) cultivars under salt stress. Journal of Experimental Botany, 1999, 50: 1701-1708
[7] 张毅, 石玉, 胡晓辉, 等. 外源Spd对盐碱胁迫下番茄幼苗氮代谢及主要矿质元素含量的影响. 应用生态学报, 2013, 24(5): 1401-1408
[8] de la Torre-González A, Navarro-León E, Blasco B, et al. Nitrogen and photorespiration pathways, salt stress genotypic tolerance effects in tomato plants (Solanum lycopersicum L.). Acta Physiologiae Plantarum, 2019, 42: 21-32
[9] López-Bucio J, Pelagio-Flores R, Herrera-Estrella A. Trichoderma as biostimulant: Exploiting the multilevel properties of a plant beneficial fungus. Scientia Horticulturae, 2015, 196: 109-123
[10] Rawat L, Singh Y, Shukla N, et al. Alleviation of the adverse effects of salinity stress in wheat (Triticum aestivum L.) by seed biopriming with salinity tolerant isolates of Trichoderma harzianum. Plant and Soil, 2011, 347: 387-400
[11] Kumar K, Manigundan K, Amaresan N. Influence of salt tolerant Trichoderma spp. on growth of maize (Zea mays) under different salinity conditions. Journal of Basic Microbiology, 2017, 57: 141-150
[12] Oljira AM, Hussain T, Waghmode TR, et al. Trichoderma enhances net photosynthesis, water use efficiency, and growth of wheat (Triticum aestivum L.) under salt stress. Microorganisms, 2020, 8: 1565
[13] Zhang F, Wang Y, Liu C, et al. Trichoderma harzianum mitigates salt stress in cucumber via multiple responses. Ecotoxicology and Environmental Safety, 2019, 170: 436-445
[14] Chen LH, Zheng JH, Shao XH, et al. Effects of Trichoderma harzianum T83 on Suaeda salsa L. in coastal saline soil. Ecological Engineering, 2016, 91: 58-64
[15] Zhao L, Wang F, Zhang YQ, et al. Involvement of Trichoderma asperellum strain T6 in regulating iron acquisition in plants. Journal of Basic Microbiology, 2014, 54: 115-124
[16] Zhao L, Liu Q, Zhang YQ, et al. Effect of acid phosphatase produced by Trichoderma asperellum Q1 on growth of Arabidopsis under salt stress. Journal of Integrative Agriculture, 2017, 16: 1341-1346
[17] Singh BN, Padmanabh D, Sarma BK, et al. Trichoderma asperellum T42 reprograms tobacco for enhanced nitrogen utilization efficiency and plant growth when fed with N nutrients. Frontiers in Plant Science, 2018, 9: 163
[18] Visconti D, Fiorentino F, Cozzolino E, et al. Can Trichoderma-based biostimulants optimize N use efficiency and stimulate growth of leafy vegetables in greenhouse intensive cropping systems? Agronomy, 2020, 10: 121
[19] Fu J, Wang YF, Liu ZH, et al. Trichoderma asperellum alleviates the effects of saline-alkaline stress on maize seedlings via the regulation of photosynthesis and nitrogen metabolism. Plant Growth Regulation, 2018, 85: 363-374
[20] 闫秀梅, 董静洲, 王瑛. 枸杞和宁夏枸杞叶片主要活性成分含量比较研究. 食品科学, 2010, 31(1): 29-32
[21] Feng XF, An P, Guo K, et al. Growth, root compensation and ion distribution in Lycium chinense under heterogeneous salinity stress. Scientia Horticulturae, 2017, 226: 24-32
[22] Dimitrova VL, Paunov MM, Goltsev V, et al. Effect of soil salinity on growth, metal distribution and photosynthetic performance of two Lycium species. Photosynthetica, 2019, 57: 32-39
[23] 颜坤, 赵世杰, 任承钢, 等. 一种棘孢木霉及其应用. 中国, ZL201710035544.5. 2020-05-08
[24] 鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 2000: 166-168
[25] 刘鹏, 武爱莲, 王劲松, 等. 不同基因型高粱的氮效率及对低氮胁迫的生理响应. 中国农业科学, 2018, 51(16): 3074-3083
[26] Yu YC, Xu T, Li X, et al. NaCl-induced changes of ion homeostasis and nitrogen metabolism in two sweet potato (Ipomoea batatas L.) cultivars exhibit different salt tolerance at adventitious root stage. Environmental and Experimental Botany, 2016, 129: 23-36
[27] 张瑛, 李浩, 张雨萱, 等. 水稻氯酸钾抗性与硝酸还原酶NR、亚硝酸还原酶NIR的关联性研究. 中国农学通报, 2019, 35(14): 1-7
[28] 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 129-130
[29] 刘涛, 鲁剑巍, 任涛, 等. 适宜氮水平下冬油菜苗期不同叶位叶片光合氮分配特征. 中国农业科学, 2016, 49(18): 3532-3541
[30] Sandhu N, Sethi M, Kumar A, et al. Biochemical and genetic approaches improving nitrogen use efficiency in cereal crops: A review. Frontiers in Plant Science, 2021, 12: 657629
[31] 刘正祥, 张华新, 杨秀艳, 等. NaCl胁迫下沙枣幼苗生长和阳离子吸收、运输与分配特性. 生态学报, 2014, 34(2): 326-336
[32] 赵俊晔, 于振文. 施氮量对小麦强势和弱势籽粒氮素代谢及蛋白质合成的影响. 中国农业科学, 2005, 38(8): 1547-1554
[33] Singh SP, Pandey S, Mishraet N, et al. Supplementation of Trichoderma improves the alteration of nutrient allocation and transporter genes expression in rice under nutrient deficiencies. Plant Physiology and Biochemistry, 2019, 143: 351-363
[34] Ashraf M, Shahzad SM, Imtiaz M, et al. Salinity effects on nitrogen metabolism in plants-focusing on the activities of nitrogen metabolizing enzymes: A review. Journal of Plant Nutrition, 2018, 41: 1065-1081
[35] Seeman JR, Critchley C. Effects of salt stress on the growth,ion content, stomatal behaviour and photosynthetic capacity of a salt sensitive species, Phaseolus vulgaris L. Planta, 1985, 164: 151-162
[36] Guy RD, Reid DM, Krouse HR. Factors affecting ¹³C/¹²C ratios of inland halophytes. I. Controlled studies on growth and isotopic composition of Puccinellia nuttalliana. Canadian Journal of Botany, 1986, 64: 2693-2699
[37] DaMatta FM, Loos RA, Silva EA, et al. Effects of soil water deficit and nitrogen nutrition on water relations and photosynthesis of pot-grown Coffea canephora Pierre. Trees, 2002, 16: 555-558
[38] 马剑英, 陈发虎, 夏敦胜, 等. 荒漠植物红砂叶片δ¹³C值与生理指标的关系. 应用生态学报, 2008, 19(5): 1166-1171

木霉对盐渍逆境下枸杞根系氮素吸收和氮素利用效率的影响

Effects of Trichoderma on nitrogen absorption and use efficiency in Lycium chinense roots under saline stress

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