盐胁迫下解钾菌对枸杞幼苗的促生效应

doi:10.13287/j.1001-9332.202104.021

应用生态学报 ›› 2021, Vol. 32 ›› Issue (4): 1289-1297.doi: 10.13287/j.1001-9332.202104.021

盐胁迫下解钾菌对枸杞幼苗的促生效应

朱娟娟^1,2, 马海军^1,2*, 张琇^1,3, 李敏^1,2, 徐小云¹, 覃洪云¹

¹北方民族大学生物科学与工程学院, 银川 750021;
²宁夏葡萄与葡萄酒技术创新中心, 银川 750021;
³宁夏特殊生境微生物资源开发与利用重点实验室, 银川 750021

收稿日期:2020-08-04 接受日期:2021-01-13 发布日期:2021-10-25
通讯作者: *E-mail: mahaijun04ren@126.com
作者简介:朱娟娟, 女, 1981年生, 博士, 讲师。主要从事植物营养生理研究。E-mail: zjj0954@163.com
基金资助:
宁夏自然科学基金项目(2018AAC03104)和宁夏回族自治区重点研发计划项目(2018BBF2019)资助

Effects of potassium-solubilizing bacteria promoting the growth of Lycium barbarum seedlings under salt stress.

ZHU Juan-juan^1,2, MA Hai-jun^1,2*, ZHANG Xiu^1,3, LI Min^1,2, XU Xiao-yun¹, QIN Hong-yun¹

¹College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China;
²Ningxia Grape & Wine Innovation Center, Yinchuan 750021, China;
³Ningxia Key Laboratory of Special Ecological Microbial Resource & Application, Yinchuan 750021, China

Received:2020-08-04 Accepted:2021-01-13 Published:2021-10-25
Contact: *E-mail: mahaijun04ren@126.com
Supported by:
Natural Science Foundation of Ningxia (2018AAC03104) and Key Research and Development Program of Ningxia Hui Autonomous Region(2018BBF2019).

摘要/Abstract

摘要： 以‘宁杞1号’枸杞为研究对象,采用单因素随机区组试验设计,研究不添加NaCl和菌剂(CK)、100 mmol·L^-1 NaCl胁迫(NaCl)、100 mmol·L^-1 NaCl胁迫下添加解钾菌KSBGY01单菌液(NaCl-M1)、KSBGY02单菌液(NaCl-M2)及其混合菌液(NaCl-M3)对枸杞幼苗叶片叶绿素、多酚、超氧阴离子(O₂^-·)、过氧化氢(H₂O₂)和可溶性糖含量、抗氧化物酶和蔗糖代谢酶活性的影响,以探索解钾菌对盐胁迫下枸杞生理生化特性的影响。结果表明: 盐胁迫下解钾菌能够提高枸杞叶片类黄酮(FLAV)、荧光激发比花青素相对指数(FERARI)、花青素(ANTH-RB)和氮素平衡指数(NBI-G),降低O₂^-·和H₂O₂含量,增加可溶性糖含量及过氧化氢酶(CAT)、蔗糖磷酸合成酶(SPS)、蔗糖合成酶(SS)和转化酶(INV)活性。不同处理间比较,NaCl-M2处理中枸杞叶片ANTH-RB值、NBI-G值、可溶性糖含量及CAT、SPS、SS和INV活性最高;NaCl-M3处理中枸杞叶片叶绿素含量、FLAV和FERARI值最高;NaCl-M1处理中枸杞叶片超氧化物歧化酶(SOD)活性最高;不添加解钾菌的NaCl处理中枸杞叶片谷胱甘肽过氧化物酶(GSH-Px)活性最高;CK处理中枸杞叶片过氧化物酶(POD)活性最高。对枸杞幼苗14项生理生化指标进行灰色系统关联度分析表明,盐胁迫条件下添加解钾菌处理的枸杞生理生化指标加权关联度均高于CK和NaCl处理,其中NaCl-M2处理的加权关联度最高。因此,添加KSBGY02单菌液更有利于缓解盐胁迫对枸杞幼苗的影响。

关键词: 解钾菌, 盐胁迫, 生理特性, 抗氧化特性, 蔗糖代谢酶, 枸杞

Abstract: We investigated the effects of potassium-releasing bacteria on physiological and bioche-mical characteristics of Lycium barbarum (Cultivar Ningqi 1) under salt stress, with an experiment with treatments following randomized block design. The treatments included control (CK), 100 mmol·L^-1 NaCl stress (NaCl), 100 mmol·L^-1NaCl stress+KSBGY01 bacteria (NaCl-M1), 100 mmol·L^-1NaCl stress+KSBGY02 bacteria (NaCl-M2), and 100 mmol·L^-1NaCl stress+KSBGY01+KSBGY02 (NaCl-M3). We measued chlorophyll content, polyphenol content, superoxide anion (O₂^-·) content, hydrogen peroxide (H₂O₂) content, soluble sugar content, antioxidant enzyme activity and sucrose metabolic enzyme activity of Lycium barbarum seedlings. Results showed that the presence of potassium bacteria increased the values of flavonoids (FLAV), fluorescence excitation than anthocyanins relative index (FERARI), anthocyanins (ANTH-RB), nitrogen balance index (NBI-G), decreased the contents of O₂^-· and H₂O₂, and improved soluble sugar content, catalase (CAT) activity, sucrose phosphate synthase (SPS) activity, sucrose synthase (SS) activity and invertase (INV) activity of leaves in Lycium barbarum seedlings under salt stress. Among all the treatments, the highest values of ANTH-RB and NBI-G, soluble sugar content, and activities of CAT, SPS, SS, and INV presented in NaCl-M2 treatment, the highest values of SPAD, FLAV, and FERARI presented in NaCl-M3 treatment, the highest activity of superoxide dismutase (SOD) presented in NaCl-M1 treatment, the highest activity of glutathione peroxidase (GSH-Px) presented in NaCl treatment, and the highest peroxisome (POD) activity presented in CK. The 14 significant physiological and biochemical indicators in the leaves of L. barbarum seedling were analyzed by grey system correlation degree method. Our results suggested that the weighted correlation degree of phy-siological and biochemical indices of L. barbarum inoculated potassium-solubilizing bacteria was higher than that under CK and NaCl treatments. The highest weighted correlation was observed in NaCl-M2 treatment. Therefore, adding KSBGY02 potassium-solubilizing bacteria could alleviate the salt stress for L. barbarum seedlings.

Key words: potassium solubilizing bacteria, salt stress, physiological characteristic, antioxidant property, sucrose-metabolizing enzyme, Lycium barbarum

朱娟娟, 马海军, 张琇, 李敏, 徐小云, 覃洪云. 盐胁迫下解钾菌对枸杞幼苗的促生效应[J]. 应用生态学报, 2021, 32(4): 1289-1297.

ZHU Juan-juan, MA Hai-jun, ZHANG Xiu, LI Min, XU Xiao-yun, QIN Hong-yun. Effects of potassium-solubilizing bacteria promoting the growth of Lycium barbarum seedlings under salt stress.[J]. Chinese Journal of Applied Ecology, 2021, 32(4): 1289-1297.

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盐胁迫下解钾菌对枸杞幼苗的促生效应

Effects of potassium-solubilizing bacteria promoting the growth of Lycium barbarum seedlings under salt stress.

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