包头市公园林下土壤外生菌根真菌的多样性与群落构建机制

doi:10.13287/j.1001-9332.202305.007

应用生态学报 ›› 2023, Vol. 34 ›› Issue (5): 1225-1234.doi: 10.13287/j.1001-9332.202305.007

包头市公园林下土壤外生菌根真菌的多样性与群落构建机制

王永龙¹, 张旋^1,2, 徐颖¹, 赵艳玲¹, 王嘉琦¹, 张煜佳¹, 杨颜慈^1*

¹包头师范学院, 生物科学与技术学院, 内蒙古包头 014030;
²中国科学院微生物研究所, 中国科学院病原微生物与免疫学重点实验室, 北京 100101

收稿日期:2023-01-13 接受日期:2023-03-07 出版日期:2023-05-15 发布日期:2023-11-15
通讯作者: *E-mail: yycjyl@163.com
作者简介:王永龙, 男, 1990年生, 副教授。主要从事外生菌根真菌的多样性与群落学研究。E-mail: wylongceltics@163.com
基金资助:
包头师范学院科研项目(BSYKJ2021-ZQ01)、国家自然科学基金项目(32260027)和内蒙古自然科学基金项目(2021BS03027)

Diversity and community assembly mechanism of soil ectomycorrhizal fungi in urban parks of Baotou City, China

WANG Yonglong¹, ZHANG Xuan^1,2, XU Ying¹, ZHAO Yanling¹, WANG Jiaqi¹, ZHANG Yujia¹, YANG Yanci^1*

¹Faculty of Biological Science and Technology, Baotou Teacher's College, Baotou 014030, Inner Mongolia, China;
²CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Received:2023-01-13 Accepted:2023-03-07 Online:2023-05-15 Published:2023-11-15

摘要/Abstract

摘要： 外生菌根(EM)真菌在森林生态系统中起重要作用。然而,人们对受人类活动密切影响的城市森林公园中土壤EM真菌的多样性、群落组成及维持机制还知之甚少。本文基于Illumina高通量测序对包头市奥林匹克公园、劳动公园和阿尔丁植物园3个典型的森林公园土壤中的EM真菌群落进行研究。结果表明: 3个公园土壤EM真菌的丰富度指数大小为劳动公园(146.43±25.17)> 阿尔丁植物园(102.71±15.31)> 奥林匹克公园(68.86±6.83);红菇属、地孔菌属、丝盖伞属、棉革菌属、粘滑菇属、蜡壳耳属、鹅膏属、须腹菌属、阿太菌属和乳菇属是3个公园的优势属;3个公园间EM真菌群落组成存在显著差异,线性判别分析效应大小(LEfSe)结果表明,所有公园都具有多度差异显著的标志EM真菌;标准化随机率(NST)和基于系统发育结合的零模型推断群落构建机制(iCAMP)分析显示,城市森林土壤EM真菌群落的构建由随机性过程和确定性过程共同驱动,但随机性过程占主导地位。随机过程中的漂变、扩散限制以及确定性过程中的同质化选择是这3个城市公园林下土壤EM真菌群落构建的主要生态学过程。

关键词: 包头市, 城市森林公园, 外生菌根真菌, 生态过程

Abstract: Ectomycorrhizal (EM) fungi play an important role in forest ecosystems. However, little is known about the mechanisms driving diversity and community composition of soil EM fungi in urban forest parks which are intensively affected by anthropogenic activities. In this study, we investigated the EM fungal community using Illumina high-throughput sequencing with soil samples collected from three typical forest parks, including Olympic Park, Laodong Park, and Aerding Botanical Garden of Baotou City. The results showed that soil EM fungi richness index followed a pattern of Laodong Park (146.43±25.17) > Aerding Botanical Garden (102.71±15.31) > Olympic Park (68.86±6.83). Russula, Geopora, Inocybe, Tomentella, Hebeloma, Sebacina, Amanita, Rhizopogon, Amphinema, and Lactarius were the dominant genera in the three parks. EM fungal community composition was significantly different among the three parks. Results of linear discriminant analysis effect size (LEfSe) indicated that all parks had biomarker EM fungi that exhibiting significantly different abundance. The normalized stochasticity ratio (NST) and the inferring community assembly mechanisms by phylogenetic-bin-based null model analysis (iCAMP) showed that both stochastic and deterministic processes determined soil EM fungal communities in the three urban parks, with a dominant role of the stochastic process. Drift and dispersal limitation in the stochastic process and homogeneous selection in the deterministic process were the dominant ecological processes of soil EM fungal community assembly in the three urban parks.

Key words: Baotou City, urban forest park, ectomycorrhizal fungi, ecological process

王永龙, 张旋, 徐颖, 赵艳玲, 王嘉琦, 张煜佳, 杨颜慈. 包头市公园林下土壤外生菌根真菌的多样性与群落构建机制[J]. 应用生态学报, 2023, 34(5): 1225-1234.

WANG Yonglong, ZHANG Xuan, XU Ying, ZHAO Yanling, WANG Jiaqi, ZHANG Yujia, YANG Yanci. Diversity and community assembly mechanism of soil ectomycorrhizal fungi in urban parks of Baotou City, China[J]. Chinese Journal of Applied Ecology, 2023, 34(5): 1225-1234.

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包头市公园林下土壤外生菌根真菌的多样性与群落构建机制

Diversity and community assembly mechanism of soil ectomycorrhizal fungi in urban parks of Baotou City, China

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