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亚致死剂量阿维菌素对家蚕食物利用及中肠消化酶活性的影响

朱九生1,2;王静1;高海燕1,2;秦曙1;乔雄梧1;韩巨才2   

  1. 1山西省农业科学院山西省农药重点实验室, 太原 030031; 2山西农业大学农学院, 山西太谷 030801
  • 收稿日期:2008-03-13 修回日期:1900-01-01 出版日期:2008-11-20 发布日期:2008-11-20

Effects of sub-lethal dosages abamectin on food intake and digestive enzyme activities of silkworm Bombyx mori L.

ZHU Jiu-sheng1,2;WANG Jing1;GAO Hai-yan1,2;QIN Shu1;QIAO Xiong-wu1;HAN Ju-cai2   

  1. 1Shanxi Key Laboratory of Pesticide Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China; 2College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi, China
  • Received:2008-03-13 Revised:1900-01-01 Online:2008-11-20 Published:2008-11-20

摘要: 采用质量法和酶活力测定法研究了阿维菌素3种亚致死剂量(LC5、LC10和LC20)处理对家蚕5 龄幼虫的食物利用及中肠3种消化酶活性的影响.结果表明:亚致死剂量阿维菌素处理对家蚕5龄幼虫的生长和食物利用有明显的抑制作用,体质量及其增加量、相对生长率均显著低于对照,同时伴随着取食量、相对取食量和排粪量减少,食物利用率和食物转化率显著降低,而近似消化率升高.阿维菌素处理的家蚕,其中肠淀粉酶和蔗糖酶活性前期受到抑制且持续时间较长,但随着幼虫的生长发育,逐渐恢复正常或高于对照;而海藻糖酶活性前期受抑制时间较短,随后活性迅速增强,并维持在与对照同一水平或高于对照.这些变化说明亚致死剂量阿维菌素对家蚕仍有一定的毒性,其程度随剂量的提高而增强,可导致家蚕消化系统紊乱,进而影响其生长发育和对食物的利用.

关键词: 曝气, 铁膜, 水禾, 净化, 铁离子

Abstract: Mulberry leaves treated with sub-lethal dosages (LC5, LC10 and LC20) abamectin were fed to the 5th instar larvae of silkworm (Bombyx mori L.), and the food intake and digestive enzyme activities of the larvae were studied by using gravimetric method and measuring enzyme activities. The results showed that sub-lethal dosages abamectin significantly inhibited the growth and food intake of the larvae, with their body mass and its increase rate as well as their relative growth rate being significantly lower than the control, and accompanied with the decreases of food intake, its relative consumption rate, and feces amount. The efficiency of the conversion of ingested food (ECI) and that of the conversion of digested food (EDI) also reduced, but the approximate digestibility (AD) increased significantly. The amylase and sucrase activities in the midgut of the larvae treated with abamectin decreased significantly for a longer time at the beginning, and then recovered to the same as or a higher level than the control, whereas the trehalase activity decreased significantly for a shorter time at the beginning, then increased significantly, and finally recovered to the normal. It was suggested that sub-lethal dosages abamectin had definite toxicity to the silkworm, and the toxic effect was increased with increasing dosage, which could result in the turbulence of silkworm’s digestive system, and further, affect its food intake and its growth and development.

Key words: aeration, Hygroryza aristata, purification, iron plaque, iron ion