氧化石墨烯对芸豆花期碳氮代谢及生长的影响

doi:10.13287/j.1001-9332.202407.013

应用生态学报 ›› 2024, Vol. 35 ›› Issue (7): 1843-1849.doi: 10.13287/j.1001-9332.202407.013

氧化石墨烯对芸豆花期碳氮代谢及生长的影响

于洋¹, 孙旭冉¹, 王诗雅¹, 李沐恺¹, 孙海燕^1,2, 郭伟^1,3*

¹黑龙江八一农垦大学农学院, 黑龙江大庆 163319;
²黑龙江省现代农业栽培技术与作物种质改良重点实验室, 黑龙江大庆 163319;
³农业农村部东北平原农业绿色低碳重点实验室, 黑龙江大庆 163319

收稿日期:2024-03-04 修回日期:2024-05-15 出版日期:2024-07-18 发布日期:2025-01-18
通讯作者: *E-mail: agrigw@163.com
作者简介:于洋, 女, 2001年生, 硕士研究生。主要从事作物生理生态与品质研究。E-mail: aprilo@126.com
基金资助:
黑龙江八一农垦大学“揭榜挂帅”科技攻关项目(JB20220001)和黑龙江省大学生创新训练计划项目(S202310223119)

Effect of graphene oxide on carbon and nitrogen metabolism and growth of kidney bean at flowering stage

YU Yang¹, SUN Xuran¹, WANG Shiya¹, LI Mukai¹, SUN Haiyan^1,2, GUO Wei^1,3*

¹College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China;
²Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement, Daqing 163319, Heilongjiang, China;
³Key Laboratory of Low-carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs, Daqing 163319, Heilongjiang, China

Received:2024-03-04 Revised:2024-05-15 Online:2024-07-18 Published:2025-01-18

摘要/Abstract

摘要： 氧化石墨烯(GO)作为一种新型纳米材料已经应用于不同领域,但作为叶面肥在农业领域的应用较少。本研究通过盆栽试验,分析了始花期叶面喷施0(CK)、50(T₁)、100(T₂)、150(T₃)和200 mg·L^-1(T₄)GO对芸豆植株形态建成和碳氮代谢的影响,以明确叶面喷施GO的生理效应。结果表明: T₁～T₄处理可不同程度地提高芸豆干物质积累量、光合色素含量和可溶性糖含量,较CK分别显著提高40.7%～43.4%、10.4%～80.7%和6.4%～9.1%,且T₃处理影响效果最佳。与CK相比,T₃和T₄处理下蔗糖磷酸合成酶、酸性转化酶和中性转化酶活性分别显著增加25.7%～45.5%、17.4%～28.6%和14.7%～20.1%,T₂和T₃处理下硝酸还原酶、谷氨酰胺合成酶和谷氨酸合成酶活性分别显著增加8.1%～15.2%、11.5%～25.0%和89.7%～93.1%。综上,芸豆始花期叶面喷施适宜浓度的GO可增加光合色素含量,提高植株光合碳、氮代谢水平,进而促进干物质积累,本研究中T₃(150 mg·L^-1)处理的作用效果最好。

关键词: 芸豆, 氧化石墨烯, 碳代谢, 氮代谢

Abstract: Graphene oxide (GO) is a novel nanomaterial being applied in different fields, but was less used as foliar fertilizer in agriculture. We conducted a pot experiment to analyze the effects of foliar spraying GO from 0 (control), 50 (T₁), 100 (T₂), 150 (T₃) and 200 mg·L^-1 (T₄) on the morphogenesis and carbon and nitrogen metabolism of kidney bean plants during the initiation of flowering to clarify the physiological effects of foliar spraying GO. The results showed that dry matter accumulation, the content of photosynthetic pigments, soluble sugars of T₁ to T₄ treatments, were significantly increased by 40.7%-43.4%, 10.4%-80.7%, 6.4%-9.1% in kidney bean plants compared with CK treatment, respectively. T₃ treatment performed the best. Meanwhile, the activities of sucrose phosphate synthase, acid converting enzyme and neutral converting enzyme of T₃ and T₄ treatments were increased by 25.7%-45.5%, 17.4%-28.6%, and 14.7%-20.1%, and the activities of nitrate reductase, glutamine synthetase, and glutamate synthetase of T₂ and T₃ treatments were increased by 8.1%-15.2%, 11.5%-25.0%, and 89.7%-93.1%, respectively. In conclusion, foliar spraying of appropriate GO in early flowering stage of kidney bean could increase the content of photosynthetic pigments, improve the level of photosynthetic carbon and nitrogen metabolism, and increase dry matter accumulation. T₃ treatment (150 mg·L^-1) was the most effective in this study.

Key words: kidney bean, graphene oxide, carbon metabolism, nitrogen metabolism

于洋, 孙旭冉, 王诗雅, 李沐恺, 孙海燕, 郭伟. 氧化石墨烯对芸豆花期碳氮代谢及生长的影响[J]. 应用生态学报, 2024, 35(7): 1843-1849.

YU Yang, SUN Xuran, WANG Shiya, LI Mukai, SUN Haiyan, GUO Wei. Effect of graphene oxide on carbon and nitrogen metabolism and growth of kidney bean at flowering stage[J]. Chinese Journal of Applied Ecology, 2024, 35(7): 1843-1849.

参考文献

[1] Díez-Pascual AM. Carbon-based nanomaterials. International Journal of Molecular Sciences, 2021, 22: 7726
[2] Dreyer DR, Park S, Bielawski CW, et al. The chemistry of graphene oxide. Chemical Society Reviews, 2010, 39: 228-240
[3] Liu LJ, Weng YN, Fang J, et al. Understanding the effect of GO on nitrogen assimilation in wheat through transcriptomics and metabolic process analysis. Chemosphere, 2022, 296: 134000
[4] 王晓静, 赵树兰, 多立安. 氧化石墨烯拌种对高羊茅种子萌发与幼苗生长的影响. 种子, 2018, 37(4): 1-4, 10
[5] 朱先贵, 赵树兰, 多立安. 氧化石墨烯对紫花苜蓿幼苗生理生态特征的影响. 天津师范大学学报: 自然科学版, 2020, 40(3): 33-37
[6] Park S, Choi KS, Kim S, et al. Graphene oxide-assisted promotion of plant growth and stability. Nanomaterials, 2020, 10: 758
[7] Zhang M, Gao B, Chen JJ, et al. Effects of graphene on seed germination and seedling growth. Journal of Nano-particle Research, 2015, 17: 78
[8] Vochita G, Oprica L, Gherghel D, et al. Graphene oxide effects in early ontogenetic stages of Triticum aestivum L. seedlings. Ecotoxicology and Environmental Safety, 2019, 181: 345-352
[9] Chen JZ, Mu QL, Tian XH. Phytotoxicity of graphene oxide on rice plants is concentration-dependent. Mate-rials Express, 2019, 9: 635-640
[10] 刘扬扬, 洪莹, 丁梦嘉, 等. 氧化石墨烯对高羊茅光合固碳特征的影响. 天津师范大学学报: 自然科学版, 2023, 43(2): 41-47
[11] Gao ML, Xu YL, Chang XP, et al. Effects of foliar application of graphene oxide on cadmium uptake by lettuce. Journal of Hazardous Materials, 2020, 398: 122859
[12] Gao ML, Chang XP, Yang YJ, et al. Foliar graphene oxide treatment increases photosynthetic capacity and reduces oxidative stress in cadmium-stressed lettuce. Plant Physiology and Biochemistry, 2020, 154: 287-294
[13] Xiao XL, Wang XP, Liu LX, et al. Effects of three graphene-based materials on the growth and photosynthesis of Brassica napus L. Ecotoxicology and Environmental Safety, 2022, 234: 113383
[14] 郑虹君, 朱叙丞, 李耀基, 等. 白芸豆功能成分、生物活性及其产品开发研究进展. 中国粮油学报, 2022, 37(12): 277-285
[15] 周超, 周传余, 徐婷, 等. 腐植酸液体叶面肥对大棚茄子产量与品质的影响. 安徽农学通报, 2014, 20(增刊2): 57-58
[16] 董丽丽, 邢翠娟, 祁瑞芳, 等. 氧化石墨烯制备条件及其对其性状的影响. 山东化工, 2018, 47(7): 13-15
[17] Lichtenthaler HK. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology, 1987, 148: 350-382
[18] Huber SC, Israel DW. Biochemical basis for partitioning of photosynthetically fixed carbon between starch and sucrose in soybean (Glycine max Merr.) leaves. Plant Physiology, 1982, 69: 691-696
[19] 汤章城. 现代植物生理学实验指南. 北京: 科学出版社, 1999
[20] 池敏青, 黄小凤, 谢和霞, 等. 木薯叶片可溶性糖含量与块根淀粉积累的关系. 中国农学通报, 2006, 22(8): 289-291
[21] 王学奎. 植物生理生化实验原理和技术. 第2版. 北京: 高等教育出版社, 2006
[22] 孔祥生. 植物生理学实验技术. 北京: 科学出版社, 2008
[23] 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000
[24] 高存斌, 张冠琳, 刘玉真. 氧化石墨烯暴露对水稻和小麦发芽及幼苗生长的影响. 土壤科学, 2019, 7(4): 251-261
[25] Guo XH, Zhao JG, Wang RM, et al. Effects of graphene oxide on tomato growth in different stages. Plant Physiology and Biochemistry, 2021, 162: 447-455
[26] 王晶, 伏兵哲, 李淑霞, 等. 外源褪黑素对干旱胁迫下沙芦草幼苗生长和生理特性的影响. 应用生态学报, 2023, 34(11): 2947-2957
[27] Siddiqui ZA, Parveen A, Ahmad L, et al. Effects of graphene oxide and zinc oxide nanoparticles on growth, chlorophyll, carotenoids, proline contents and diseases of carrot. Scientia Horticulturae, 2019, 249: 374-382
[28] Hu YM, Zhang P, Zhang X, et al. Multi-wall carbon nanotubes promote the growth of maize (Zea mays) by regulating carbon and nitrogen metabolism in leaves. Journal of Agricultural and Food Chemistry, 2021, 69: 4981-4991
[29] Dasmahapatra AK, Tchounwou PB. Histopathological evaluation of the interrenal gland (adrenal homolog) of Japanese medaka (Oryzias latipes) exposed to graphene oxide. Environmental Toxicology, 2022, 37: 2460-2482
[30] 姜东, 于振文, 李永庚, 等. 施氮水平对高产小麦蔗糖含量和光合产物分配及籽粒淀粉积累的影响. 中国农业科学, 2002, 35(2): 157-162
[31] 张智猛, 万书波, 宁堂原, 等. 不同花生品种氮代谢相关酶活性的研究. 植物营养与肥料学报, 2007, 13(4): 707-713
[32] Cruz C, Lips SH, Martins-Loução MA. The effect of nitrogen source on photosynthesis of carob at high CO₂ concentrations. Physiologia Plantarum, 1993, 89: 552-556
[33] 邢兴华, 徐泽俊, 齐玉军, 等. 外源α-萘乙酸对花期干旱大豆碳代谢的影响. 应用生态学报, 2018, 29(4): 1215-1224
[34] Younes NA, Dawood MFA, Wardany AA. Biosafety assessment of graphene nanosheets on leaf ultrastructure, physiological and yield traits of Capsicum annuum L. and Solanum melongena L. Chemosphere, 2019, 228: 318-327
[35] Zhao SL, Wang W, Chen XJ, et al. Graphene oxide affected root growth, anatomy, and nutrient uptake in alfalfa. Ecotoxicology and Environmental Safety, 2023, 250: 114483
[36] Cheng YY, Wang Y, Han YL, et al. The stimulatory effects of nanochitin whisker on carbon and nitrogen metabolism and on the enhancement of grain yield and crude protein of winter wheat. Molecules, 2019, 24: 1752
[37] Winkler AJ, Dominguez-Nuñez JA, Aranaz I, et al. Short-chain chitin oligomers: Promoters of plant growth. Marine Drugs, 2017, 15: 40

氧化石墨烯对芸豆花期碳氮代谢及生长的影响

Effect of graphene oxide on carbon and nitrogen metabolism and growth of kidney bean at flowering stage

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李晶, 陈松林, 李晨欣, 周萍. 基于生态网络分析的厦漳泉地区土地碳代谢 [J]. 应用生态学报, 2023, 34(5): 1375-1383.
[2]	杨雅丽, 马雪松, 解宏图, 鲍雪莲, 梁超, 朱雪峰, 何红波, 张旭东. 保护性耕作对土壤微生物群落及其介导的碳循环功能的影响 [J]. 应用生态学报, 2021, 32(8): 2675-2684.
[3]	赵庆玲,孙敏,林文,任爱霞,王志鑫,张蓉蓉,李蕾,高志强. 播种方式对旱地小麦土壤水分变化和籽粒蛋白质形成的影响 [J]. 应用生态学报, 2021, 32(11): 3977-3987.
[4]	赵佳冰, 杜常健, 马长明, 孙佳成, 韩振泰, 严东辉, 江泽平, 史胜青. 板栗“燕山早丰”幼苗光合与碳氮代谢对干旱胁迫的响应 [J]. 应用生态学报, 2020, 31(11): 3674-3680.
[5]	宁宇,艾希珍,李清明,毕焕改. 光质对韭菜碳氮代谢、生长和品质的影响 [J]. 应用生态学报, 2019, 30(1): 251-258.
[6]	王月,滕志远,张秀丽,车延辉,孙广玉. 大气NO₂影响植物生长与代谢的研究进展 [J]. 应用生态学报, 2019, 30(1): 316-324.
[7]	王永慧, 陈建平, 高进, 张祥, 陈源, 陈德华. 盐胁迫对Bt棉棉蕾杀虫蛋白表达的影响 [J]. 应用生态学报, 2018, 29(9): 3017-3023.
[8]	谷端银, 王秀峰, 高俊杰, 焦娟, 刘中良, 张艳艳. 纯化腐植酸对氮胁迫下黄瓜幼苗生长和氮代谢的影响 [J]. 应用生态学报, 2018, 29(8): 2575-2582.
[9]	王永慧, 陈建平, 孙艳茹, 张祥, 陈德华. 土壤盐分对Bt棉铃壳中Bt蛋白含量和氮代谢生理的影响 [J]. 应用生态学报, 2018, 29(8): 2583-2589.
[10]	邢兴华, 徐泽俊, 齐玉军, 王晓军, 孙东雷, 卞能飞, 王幸. 外源α-萘乙酸对花期干旱大豆碳代谢的影响 [J]. 应用生态学报, 2018, 29(4): 1215-1224.
[11]	张小翠, 刘玉梅, 白龙强, 贺超兴, 于贤昌, 李衍素. 亚适宜温光环境下不同硝铵比营养液对黄瓜幼苗生长、氮吸收和代谢的影响 [J]. 应用生态学报, 2016, 27(8): 2527-2534.
[12]	井大炜,邢尚军,刘方春,马海林,杜振宇,马丙尧,于学斗,朱亚萍. 保水剂-尿素凝胶对侧柏裸根苗细根生长和氮素利用率的影响 [J]. 应用生态学报, 2016, 27(4): 1046-1052.
[13]	高青海*,贾双双,苗永美,陆晓民,李慧敏. 亚低温条件下外源褪黑素对甜瓜幼苗氮代谢及渗透调节物质的影响 [J]. 应用生态学报, 2016, 27(2): 519-524.
[14]	甄爱, 胡晓辉, 任文奇, 苏春杰, 靳晓青, 孙先鹏. 外源γ-氨基丁酸对Ca(NO₃)₂胁迫下甜瓜幼苗NO₃^--N同化的影响 [J]. 应用生态学报, 2016, 27(12): 3987-3995.
[15]	韩宇睿1,王秀峰1,2**,杨凤娟1,2,魏珉1,2,史庆华1,2,李清明1,2,崔秀敏1,2. NO₃-胁迫对草莓幼苗光合特性和氮代谢的影响 [J]. 应用生态学报, 2015, 26(8): 2314-2320.