高温胁迫对根瘤菌Tn5在土壤中的存活及其表型表达的影响

应用生态学报 ›› 1995, Vol. 6 ›› Issue (4): 411-416.

高温胁迫对根瘤菌Tn5在土壤中的存活及其表型表达的影响

蔺继尚, 崔明学, 靳素英, 李宁, 李明祺, 赵巍, 张晓东

中国科学院沈阳应用生态研究所陆地生态系统痕量物质生态过程开放实验室, 沈阳 110015

收稿日期:1995-01-23 修回日期:1995-05-28 出版日期:1995-10-25 发布日期:1995-10-25
基金资助:
国家自然科学基金;中国科学院陆地生态系统痕量物质生态过程开放实验室基金

Impact of high temperature stress on survival of Tn5 mutants or Rhizobium fredii and phenotypic expression of Tn5 in soil

Lin Jishang, Cui Mingxue, Jin Suying, Li Ning, Li Mingqi, Zhao Wei, Zhang Xiaodong

Laboratory of Ecological Process of Trace Substance in Terrestrial Ecosystem, Institute of Applied Ecology, Academia Sinica, Shengyang 110015

Received:1995-01-23 Revised:1995-05-28 Online:1995-10-25 Published:1995-10-25

摘要/Abstract

摘要： 研究了3株弗氏中华根瘤菌(Rhizobiumfredii)Tns突变株于适宜温度和高温胁迫两种条件下在土壤中的存活和Tns表型的表达。在适宜温度(28℃)条件下的灭菌和未灭菌土壤中的存活研究表明生物因素抑制了突变株和野生型的生长。但野生型和突变株的存活种群密度之间无显着差异(P=0.01).在高温胁迫(40℃)条件下,土壤中野生型和突变株的种群密度迅速下降,其中部分ON-2和ON-3细胞丢失了Tns表型,说明部分细菌的Tn5表型在高温胁迫条件下不能表达。

关键词: 转座子Tn5, 监测标记, 高温胁迫, 风蚀防治, 防治机理, 聚丙烯酰胺(PAM)

Abstract: Awild type and thyee transposon Tn5 insertion mutants of Rhizobium fredii were used to study their survival and Tns phenotypic expression in soil under optimum temperature (28 C)and temperature stress (40 C).Survival studies with sterile and nonsterile soils show that under optimum temperature (28 C), biotic factors inhibit the growth of mutant and wild type.But, there is no significant difference (P=0.01) between the surviving populations of wild type and Tn5 mutants.Under temperature stressed condition (40 C), both wild type and mutant populations decline rapidly, and some of the mutant cells (ON-2 and ON-3) can't express kanamycin resistance.These results suggest that high temperature is a harmful factor for the survival of bacteria and the phenotypic expression of Tn5 in soil.

Key words: Transposon Tn5, Monitoring marker, High temperature stress, wind erosion control, polyacrylamide (PAM), control mechanism

蔺继尚, 崔明学, 靳素英, 李宁, 李明祺, 赵巍, 张晓东. 高温胁迫对根瘤菌Tn5在土壤中的存活及其表型表达的影响[J]. 应用生态学报, 1995, 6(4): 411-416.

Lin Jishang, Cui Mingxue, Jin Suying, Li Ning, Li Mingqi, Zhao Wei, Zhang Xiaodong . Impact of high temperature stress on survival of Tn5 mutants or Rhizobium fredii and phenotypic expression of Tn5 in soil[J]. Chinese Journal of Applied Ecology, 1995, 6(4): 411-416.

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