埋植17α-甲基睾酮诱导点带石斑鱼性转化技术

应用生态学报 ›› 2001, Vol. ›› Issue (2): 296-298.

埋植17α-甲基睾酮诱导点带石斑鱼性转化技术

陈国华, 张本

海南大学水产系, 海口570228

收稿日期:2000-10-30 修回日期:2000-11-29
通讯作者: 陈国华,男,1956年生,副教授,主要从事鱼类学教学、科研,发表论文近20篇．E-mail:chguh@hainu.edu.cn
基金资助:
海南省百项农业新技术项目(合同书编号1101)

Technique of implanting 17α-methyltestosterone to induce the sex reversal of Epinephelus malabaricus

CHEN Guohua, ZHANG Ben

Aquaculture Department of Hainan University, Haikou 570228

Received:2000-10-30 Revised:2000-11-29

摘要/Abstract

摘要： 论述了埋植17α-甲基睾酮诱导点带石斑鱼(Epinephelusmalabaricus)性转化的方法,包括药物的处理、用量、手术操作等.3龄的雌性点带石斑鱼经过2次埋植外源激素手术转化为功能性雄鱼,139d后58%可挤出精液,301d后100%可挤出精液.功能性雄鱼用于人工繁殖,亲鱼自然产卵,1999～2000年2年内共孵出仔鱼7103万尾.所得仔鱼用于鱼苗培育,得到发育正常的幼鱼.认为埋植外源激素诱导点带石斑鱼完成性转化的方法简便可行,能满足规模化人工繁殖的需要.

关键词: 点带石斑鱼, 性转化, 人工诱导, 激素埋植技术

Abstract: This paper discussed the method of implanting 17α-methyltestosterone to induce the sex reversal of Epinephelus malabaricus, which included chemical treatment, dosage and operating on the fish. After twice implanting of exogenous hormone, the mature females of 3 years old were transformed into functional males. Sperm could be squeezed from 58% of the fish on 139 days after operating,and from 100% on 301days. When the functional males were used for artificial breeding, the parent fish spawned in a natural way, and 71.03 million larvae were hatched in the two years of 1999～2000. The larvae were used for rearing of fish fry, from which normally growing young fish was get.It is believed that the method of implanting exogenous hormone to induce the sex reversal of E.malabaricus is easy and stable, which can satisfy the requirement of artificial breeding in factories.

Key words: Epinephelus malabaricus, Sex reversal, Artificial induction, Androgen-implantation technology

中图分类号:

S967.3

陈国华, 张本. 埋植17α-甲基睾酮诱导点带石斑鱼性转化技术[J]. 应用生态学报, 2001, (2): 296-298.

CHEN Guohua, ZHANG Ben . Technique of implanting 17α-methyltestosterone to induce the sex reversal of Epinephelus malabaricus[J]. Chinese Journal of Applied Ecology, 2001, (2): 296-298.