外源钙对不同钙敏感型番茄幼苗生理特性的影响

应用生态学报 ›› 2005, Vol. 16 ›› Issue (2): 267-272.

外源钙对不同钙敏感型番茄幼苗生理特性的影响

董彩霞^1,3, 周健民¹, 赵世杰², 王火焰¹

1. 中国科学院南京土壤研究所, 南京, 210008;
2. 山东农业大学生命科学院, 山东, 泰安, 271018;
3. 南京农业大学资源与环境学院, 南京, 210095

收稿日期:2003-11-17 修回日期:2004-06-28 出版日期:2005-02-15 发布日期:2005-02-15
通讯作者: 周健民
基金资助:
国家自然科学基金重点项目(30230250)和中国科学院南京土壤研究所所长基金资助项目(BR0007).

Effects of exogenous Ca on some physiological characteristics of tomato (Lycopersicon esculentum) seedlings with different Ca sensitivity

DONG Caixia^1,3, ZHOU Jianmin¹, ZHAO Shijie², WANG Huoyan ¹

1. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
2. College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China;
3. College of Resources and Enviromental Sciences, Nanjing Agricultural University, Nanjing 210095, China

Received:2003-11-17 Revised:2004-06-28 Online:2005-02-15 Published:2005-02-15

摘要/Abstract

摘要： 利用钙不敏感型番茄品种(江蔬1号)和钙敏感型番茄品种(L-402)为试材,研究了不同浓度的外源钙对其根系活力、钙调素含量、叶绿素a、b含量和比值及活性氧清除酶系统活性的影响.结果表明,根系钙调素(CaM)含量随介质钙浓度增加而增加,不敏感品种江蔬1号高于敏感品种L-402.根系活力、生长点和真叶chla/b比值在低钙强度下(1和4 mg·L^-1)以江蔬1号显著高于L-402,在充足供钙时(100 mg·L^-1)L-402显著高于江蔬1号,表明江蔬1号品种具有较强的耐低钙和光胁迫的能力.对活性氧清除酶系统活性来说,在三个钙浓度下敏感品种L-402的POD、CAT酶活性均显著高于江蔬1号,降钙使两酶活性升高,SOD酶活性下降,且L-402下降的幅度高于江蔬1号,表明低钙造成的胁迫对L-402品种影响较大,江蔬1号较耐受缺钙胁迫.

关键词: 钙敏感程度, 番茄幼苗, 根系活力, 叶绿素a/b, 活性氧清除酶

Abstract: Two tomato (Lycopersicon esculentum) cultivars with different Ca sensitivity were used to study the effects of exogenous Ca on their root activity,CaM content,chlorophyll a/b ratio,and reactive oxygen-scavenging enzymes' activities under different Ca concentrations in nutrient solution.The results showed that the root activity and CaM content of two cultivars increased with increasing Ca concentrations in the nutrient solution.The root activity and chlorophyll a/b ratio of Ca-insensitive cultivar Jiangshuyihao were higher than those of Ca-sensitive L-402 under extreme low Ca stress (1 and 4 mg稬^-1),while lower than those of L-402 when sufficient Ca was applied (100 mg稬^-1),indicating that Jiangshuyihao had a greater capability to bear the nutrient and light stress under low Ca stress.The SOD activity of Ca-insensitive cultivar Jiangshuyihao under 1 and 4 mg稬^-1 Ca was higher than that of L-402,while the activities of POD and CAT of L-402 were consistently higher than that of Jiangshuyihao under test Ca concentrations in the nutrient solution.Comparing with 100 mg稬^-1 Ca in solution,4 mg稬^-1 Ca led to an increase of CAT and POD activities and a decrease of SOD activity in the two cultivars,but the variation of SOD activity was greater in L-402 than in Jiangshuyihao,indicating that the system of defence enzymes in Ca-insensitive cultivar played a key role when suffered from Ca stress.

Key words: Ca sensitivity, Tomato seedlings, Root activity, Chlorophyll a/b, Reactive oxygen-scavenging enzymes

中图分类号:

Q945.3

董彩霞, 周健民, 赵世杰, 王火焰. 外源钙对不同钙敏感型番茄幼苗生理特性的影响[J]. 应用生态学报, 2005, 16(2): 267-272.

DONG Caixia, ZHOU Jianmin, ZHAO Shijie, WANG Huoyan . Effects of exogenous Ca on some physiological characteristics of tomato (Lycopersicon esculentum) seedlings with different Ca sensitivity[J]. Chinese Journal of Applied Ecology, 2005, 16(2): 267-272.

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