日本曲霉产生的黑麦酮酸F对玉米的化感作用

应用生态学报 ›› 2004, Vol. ›› Issue (1): 145-148.

日本曲霉产生的黑麦酮酸F对玉米的化感作用

曾任森, 骆世明, 施月红

华南农业大学热带亚热带生态研究所, 广州, 510642

收稿日期:2003-01-21 修回日期:2003-07-02 出版日期:2004-01-15
通讯作者: 曾任森,男,1965年8月生,博士,副教授,从事植物化学生态学研究.在国内外刊物上发表论文30多篇,其中4篇被SCI收录.E-mail:rszeng@scau.edu.cn.
基金资助:
国家自然科学基金(30270230);广东省自然科学基金(990682);广东省科技计划项目(2002C20506);中国科学院昆明植物研究所植物化学重点实验室基金资助项目

Allelopathic effects of secalonic acid F produced by Aspergillus japonicus on Zea mays

ZENG Rensen, LUO Shiming, SHI Yuehong

Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China

Received:2003-01-21 Revised:2003-07-02 Online:2004-01-15

摘要/Abstract

摘要： 日本曲霉(Aspergillus japonicus)是土壤和谷物种子表面的一种常见真菌.研究结果表明,日本曲霉所产生的大量黑麦酮酸F(SAF)对玉米(Zea mays)有很强的化感作用,低浓度显著促进玉米幼苗生长,高浓度则抑制.在0.0375mmol·L^-1SAF下,玉米幼苗根长增长31.7%,根数量增加13.2%,根活力提高4.73倍,并促进玉米对P、K、Ca、Mg、S等5种营养元素的吸收.高浓度SAF(0.3mmol·L^-1)下玉米根活力受抑制(72.1%),根对N、P、K、Ca、Mg、Fe等营养元素的吸收也受抑制.0.6mmol·L^-1的SAF下根完全失去活力.电镜观察表明,有SAF的情况下玉米叶绿体片层结构模糊、混乱,双层膜不完整.

Abstract: Aspergillus japonicus Saito is a common soil-borne fungus, and exists on the surface of some cereal crop seeds. Secalonic acid F (SAF) abstract is the main allelochemical from the fungus. Bioassays showed that SAF significantly stimulated the seedling growth of corn (Zea mays L.) abstract at a concentration of 0.0375 mmol稬^-1. The root length, root numbers and root oxidation activities increased by 31.7%, 13.2%, and 373%, respectively. Secalonic acid F also increased the nutrient absorption of P, K, Ca, Mg, and S at the same concentration. However, SAF inhibited the seedling growth of corn at concentrations of 0.3 mmol稬^-1 and 0.6 mmol稬^-1. The root length, root dry weight, and shoot length were inhibited by 27.7%, 39.1% and 35.8% by 0.3 mmol稬^-1 of SAF, and root activities were inhibited by 72.1% and 100% by 0.3 mmol稬^-1 and 0.6 mmol稬^-1 of SAF, respectively. The nutrient absorption of N, P, K, Ca, Mg and Fe was reduced when the corn seedlings were treated with 0.3 mmol稬^-1 SAF. Transmission electron microscope observations showed that the corn treated with 0.3 mmol稬^-1 of SAF had a swelling and disorderly arrangement chloroplast, and the stratiform structure of chloroplast became unconspicuous.

中图分类号:

Q948.1

曾任森, 骆世明, 施月红. 日本曲霉产生的黑麦酮酸F对玉米的化感作用[J]. 应用生态学报, 2004, (1): 145-148.

ZENG Rensen, LUO Shiming, SHI Yuehong. Allelopathic effects of secalonic acid F produced by Aspergillus japonicus on Zea mays[J]. Chinese Journal of Applied Ecology, 2004, (1): 145-148.

参考文献

[1] Aberhart, DJ, Chen YS,et al.1965.Mould metabolites Ⅳ.The isolation and constitution of some ergot pigments.Tetrahedron,21:1417～1432
[2] Andersen R, George B,et al.1977.Secalonic acids D and F are toxic metabolites of Aspergillus aculeatus.J Org Chem,42(2): 352～353
[3] Cutler HG.1999.Potentially useful natural product herbicides from microorganisms.In:Inderjit,KM,Dakshini M, Foy CL eds.Principles and Practices in Plant Ecology: Allelochemical Interactions.Boca Raton: CRC Press.497～513
[4] Fang Z-D (方中达).1998.Research Methods for Plant Pathology.Beijing: China Agricultural Press.46～50 (in Chinese)
[5] Hu J-C(胡江春), Wang S-J(王书景).1996.Study on soil sickness by soybean continuous cropping Ⅰ.Effects of mycotoxin produced by Penicillium purpurogenum.Chin J Appl Ecol(应用生态学报),7(4):396～400 (in Chinese)
[6] Hu J-C(胡江春), Xue D-L (薛德林), Wang S-J(王书景).1998.Obstacles of soybean continuous cropping Ⅱ.Mechanism of soybean yield decline and control strategies for toxin of Penicillium purpurogenum in soils.Chin J Appl Ecol(应用生态学报),9(4):429～434 (in Chinese)
[7] Institute of Soil Science,Chinese Academy of Sciences(中国科学院南京土壤研究所).1978.Physical and Chemical Analysis of Soil.Shanghai: Shanghai Science and Technology Press.227 (in Chinese)
[8] Ma T-H(马太和).1985.Soilless Culture.Beijing: Beijing Press.321 (in Chinese)
[9] Li F-D (李阜棣), Yu Z-N (喻子牛), He S-J (何绍江).1996.Experimental Technology of Agricultural Microbiology.Beijing: China Agricultural Press.32～46 (in Chinese)
[10] Nanjing Agricultural University (南京农业大学).1992.Agrochemical Analysis of Soil.Beijing: China Agricultural Press.304 (in Chinese)
[11] Qi Z-T (齐祖同).1997.Flora Fungorum sinicorum Vol.5:Aspergillus et Teleomorphi cognati.Beijing: Science Press.98～100 (in Chinese)
[12] Raper KB, Fennell DI.1965.The Genus Aspergillus.Baltimore: Williams & Wilkins.327～330
[13] Shandong Agricultural College (山东农学院),Northeast China Agricultural College (西北农学院).1980.Experimental Manual in Plant Physiology.Jinan: Shandong Science and Technology Press.179～187 (in Chinese)
[14] Steffens JC, Robeson DJ.1987. Secalonic acid A, a vivotoxin in pink root-infected onion.Phytochemistry, 26(6):1599 ～1602
[15] Steyn PS.1970.The isolation, structure and absolute configuration of secalonic acid D, toxic metabolite of Penicillium oxalicum.Tetrahedron, 26:51～57
[16] Tzean SS, Chen JL,et al.1990.Aspergillus species and relative teleomorphs from Taiwan.Hsinchu: Food Industry Research and Development Institute.43
[17] Wang BH, Polya GM, Wang BH.1996.The fungal teratogen secalonic acid D is an inhibitor of protein kinase C and of cyclic AMP-dependent proteinkinase.Planta Medica, 62(2):111～114
[18] Wei Q (韦琦), Zeng R-S(曾任森),et al.1997.Isolation and identification of allelochemicals from aerial parts of Ageritum conyziodes L.Acta Phytoecol Sin(植物生态学报),21(4): 360～366 (in Chinese)
[19] Wiliamson GB.1988.Bioassays for allelopathy measuring treatment responses with independent control.J Chem Ecol, 14(1):181～187
[20] Yamazaki M, Maebayashi Y, Miyaki K.1971.The isolation of secalonic acid A from Aspergillus ochraceus cultured on rice.Chem Pharma Bull,19:199～201
[21] Zeng RS, Luo SM, et al.2001a.Allelopathy of Aspergillus japonius SAITO on crops.Agron J, 93(1):60～64
[22] Zeng RS, Luo SM,et al.2001b.Physiological and biochemical mechanism of allelopathy of secalonic acid F on higher plants.Agron J,93(1):72～79