Ecological distribution and genetic relation of endophytic fungi in Cynomorium songaricum and its host Nitraria tangutorum

doi:10.13287/j.1001-9332.201703.008

Abstract

Abstract: This is the first report of the distribution and genetic relationships of endophytic fungi from the parasitic plant Cynomorium songaricum and its host Nitraria tangutorum. Endophytic fungi from the root of natural N. tangutorum, parasitic plant C. songaricum and its host N. tangutorum were isolated by tissue culture, and they were identified by morphology combined with molecular barcoding based on ITS-rDNA sequence. The isolation rates, colonization rates, isolation frequency, diversity index, evenness index, similarity coefficient and genetic relationships among fungal taxa were estimated by phylogenetic analysis, and differences in fungal endophyte distribution were investigated. The results showed that a total of 49 isolates were obtained belonging to 18 different taxa. 95.9% of these taxa were in Ascomycota, and the remaining was in Basidiomycota (4.1%). The isolation rate and colonization rate of endophytic fungi were 15.3% and 25.0%, respectively. The Shannon biodiversity index was the highest in the root of natural N. tangutorum at 2.13. The simila-rity coefficient was highest between the stem of C. songaricum and the flower of C. songaricum at0.50. Fusarium was the dominant genus in N. tangutorum, while Penicillium was the primary genus in C. songaricum. The differential distribution of fungal taxa between N. tangutorum and C. songaricum suggested that the parasitic relationship influences the endophytic fungal community.

Key words: medicinal plant, endophyte, interaction, parasitic plant

WANG Meng-liang, JIA Yan, CUI Jin-long, WANG Jun-hong. Ecological distribution and genetic relation of endophytic fungi in Cynomorium songaricum and its host Nitraria tangutorum[J]. Chinese Journal of Applied Ecology, 2017, 28(3): 815-820.

References

[1] Meng HC, Wang S, Li Y, et al. Chemical constituents and pharmacologic actions of Cynomorium plants. Chinese Journal of Natural Medicines, 2013, 11: 321-329
[2] Pharmacopoeia Commission of the People’s Republic of China. Pharmacopoeia of the People’s Republic of China (Ⅰ). Beijing: China Medicine Science and Techno-logy Press, 2010: 325 (in Chinese)
[3] Cui ZH, Guo ZQ, Miao JH, et al. The genus Cynomo-rium in China: An ethnopharmacological and phytochemical review. Journal of Ethnopharmacology, 2013, 147: 1-15
[4] Vahabi K, Sherameti I, Bakshi M, et al. The interaction of Arabidopsis with Piriformospora indica shifts from initial transient stress induced by fungus-released chemical mediators to a mutualistic interaction after physical contact of the two symbionts. BMC Plant Biology, 2015, 15: 61-69
[5] Zhang S-P (张少鹏), Xu T (胥婷), Yang L-Q (杨丽强), et al. Endophytic fungal communities of Stipa sp. roots in different types of steppes in northern China. Chinese Journal of Applied Ecology (应用生态学报), 2014, 25(12): 3475-3482 (in Chinese)
[6] Wang Y (王宇), Dai C-C (戴传超), Chen Y (陈晏). Antimicrobial activity of volatile oil from Atractylodes lancea against three species of endophytic fungi and seven species of exogenous fungi. Chinese Journal of Applied Ecology (应用生态学报), 2009, 20(11): 2778-2784 (in Chinese)
[7] Agler MT, Ruhe J, Kroll S, et al. Microbial hub taxa link host and abiotic factors to plant microbiome variation. Plos Biology, 2016, 14(1): e1002352
[8] Zhou SL, Yan SZ, Wu ZY, et al. Endophytic fungi associated with Macrosolen tricolor and its host Camellia oleifera. World Journal of Microbiology and Biotechnology, 2014, 30: 1775-1784
[9] Cui JL, Guo TT, Ren ZX, et al. Diversity and antioxidant activity of culturable endophytic fungi from alpine plants of Rhodiola crenulata, R. angusta, and R. sachalinensis. PLoS One, 2015, 10(3): e0118204
[10] Wei J-C (魏景超). Handbook of Fungal Identification. Shanghai: Shanghai science and Technology Press, 1979 (in Chinese)
[11] Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 1980, 16: 111-120
[12] Wu J-R (伍建榕), Han S-F (韩素芬), Wang G-P (王光萍), et al. Advance in studies of Orchidaceae mycorrhiza. Journal of Southwest Forestry College (西南林学院学报), 2004, 24(3): 76-81 (in Chinese)
[13] Wu J-R (伍建榕), Han S-F (韩素芬), Zhu Y-Y (朱有勇), et al. Ultra-structure of symbiosis mycorrhizal between Cymbidium goeringii and Rhizoctonia sp. Journal of Nanjing Forestry University (Natural Science) (南京林业大学学报:自然科学版), 2005, 29(4): 105-109 (in Chinese)
[14] Vega CD, Arista M, Ortiz PL, et al. Anatomical relations among endophytic holoparasitic angiosperms, autotrophic host plants and mycorrhizal fungi: A novel pripartite interaction. American Journal of Botany, 2010, 97: 730-737
[15] Akiyama K, Matsuzaki K, Hayashi H. Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature, 2005, 435: 824-827
[16] Ding Z-S (丁志山), Jiang F-S (蒋福升), Jin B (金波), et al. Study function of endophytic fungus in parasitism process of mistlet. China Journal of Chinese Materia Medica (中国中药杂志), 2008, 33(11): 1243-1246 (in Chinese)
[17] Arnold AE, Lutzoni F. Diversity and host range of foliar fungal endophytes: Are tropical leaves biodiversity hotspots? Ecology, 2007, 88: 541-549
[18] Wani ZA, Ashraf N, Mohiuddin T, et al. Plant-endophyte symbiosis, an ecological perspective. Applied Microbiology and Biotechnology, 2015, 99: 2955-2965
[19] Chang Y-X (常艳旭), Su G-E (苏格尔). The effective components and index components of Cynomorium songaricum. Research and Practice on Chinese Medicine (现代中药研究与实践), 2015, 19(3): 55-60 (in Chinese)
[20] Li B (李标), Li Q (李清), Tang K (唐坤), et al. Effect of endophytic fungi on modernization of traditional Chinese medicine. China Journal of Chinese Materia Medica (中国中药杂志), 2016, 41(1): 14-19 (in Chinese)