Impact of high temperature stress on survival of Tn5 mutants or Rhizobium fredii and phenotypic expression of Tn5 in soil

Abstract

Abstract: Awild type and thyee transposon Tn5 insertion mutants of Rhizobium fredii were used to study their survival and Tns phenotypic expression in soil under optimum temperature (28 C)and temperature stress (40 C).Survival studies with sterile and nonsterile soils show that under optimum temperature (28 C), biotic factors inhibit the growth of mutant and wild type.But, there is no significant difference (P=0.01) between the surviving populations of wild type and Tn5 mutants.Under temperature stressed condition (40 C), both wild type and mutant populations decline rapidly, and some of the mutant cells (ON-2 and ON-3) can't express kanamycin resistance.These results suggest that high temperature is a harmful factor for the survival of bacteria and the phenotypic expression of Tn5 in soil.

Key words: Transposon Tn5, Monitoring marker, High temperature stress, wind erosion control, polyacrylamide (PAM), control mechanism

Lin Jishang, Cui Mingxue, Jin Suying, Li Ning, Li Mingqi, Zhao Wei, Zhang Xiaodong . Impact of high temperature stress on survival of Tn5 mutants or Rhizobium fredii and phenotypic expression of Tn5 in soil[J]. Chinese Journal of Applied Ecology, 1995, 6(4): 411-416.

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