Abstract: Many active substances such as organic acids and enzymes excreted by living plant roots could induce a great difference of Eh and pH values between root-soil interface and non?rhizosphere soil,forming a special root-soil interface miniature environment.As a mini-type ecological area with most frequent exchanges of substances,root-soil interface plays a crucial role in their absorption,transformation,migration and eco-toxicological effects.In this paper,the eco-chemical behaviors of Cd in root-soil interface affected by the change of pH,Eh and root secretion,and its eco-toxicological effects on microorganisms and enzymes in root-soil interface were reviewed,based on the related research advances in recent decade.The shortages in relevant fields were pointed out,and the scientific problems to be researched in the future were suggested.