Isolation and identification of a phenanthrene-degrading bacterial strain

Chinese Journal of Applied Ecology

• Articles • Previous Articles Next Articles

Isolation and identification of a phenanthrene-degrading bacterial strain

ZHU Rugang¹; ZHONG Ming¹; ZHOU Qixing²; LIU Haining¹;LI Yushuang¹

¹Key Laboratory of Agricultural Biotechnology of Liaoning Province, Shenyang Agricultural
University, Shenyang 110161, China;
²Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese
Academy of Sciences, Shenyang 110016, China

Received:2005-11-03 Revised:2006-08-24 Online:2006-11-18 Published:2006-11-18

Abstract

Abstract: Through selective enriched culture, a phenanthrene-degrading bacterial strain was isolated from the oil-contaminated soil in Shenfu irrigation area of Shenyang, Northeast China. The morphological and physiological-biochemical identification, 16S rDNA sequence analysis, and phylogenetic study showed that this strain was belonged to genus Acinetobacter and named as Acinetobacter sp. L2, and closest to Acinetobacter sp. DG880［AY258108］. It could use phenanthrene as the sole carbon source. After 7 days culture, the degradation rate of phenanthrene was 96.3%. According to the activity of catechol 2,3-dioxygenase, the strain probably had phenanthrene-degrading genes.

Key words: Riverine organic carbon, Terrestrial ecosystem, Global carbon cycle, Drainage erosion, Radiocarbon, Anthropogenic activities

ZHU Rugang¹; ZHONG Ming¹; ZHOU Qixing²; LIU Haining¹;LI Yushuang¹ . Isolation and identification of a phenanthrene-degrading bacterial strain[J]. Chinese Journal of Applied Ecology.

[1]	ZHANG Tian-you, CHEN Zhi, WEN Zhong-ming, YU Gui-rui. Research advances in critical transition and its ecological mechanisms of terrestrial ecosystems. [J]. Chinese Journal of Applied Ecology, 2022, 33(3): 613-622.
[2]	YU Gui-rui, ZHAGN Li, HE Hong-lin, YANG Meng. A process-based model and simulation system of dynamic change and spatial variation in large-scale terrestrial ecosystems [J]. Chinese Journal of Applied Ecology, 2021, 32(8): 2653-2665.
[3]	YU Gui-rui, NIU Shu-li, LI Fa-dong, ZHANG Lei-ming, CHEN Wei-nan. Manipulative experiments networks on response and adaptation of terrestrial ecosystems to environmental changes: Building the research methods and technology system [J]. Chinese Journal of Applied Ecology, 2021, 32(7): 2275-2289.
[4]	YU Gui-rui, ZHANG Lei-ming, ZHANG Yang-jian, YANG Meng. A coordinated three-dimensional network for observing large-scale terrestrial ecosystem status changes and the consequences on resources and environment [J]. Chinese Journal of Applied Ecology, 2021, 32(6): 1903-1918.
[5]	YU Gui-rui, CHEN Zhi, YANG Meng, WANG Qiu-feng. Discussion on the theoretical basis and technical system of large-scale terrestrial ecosystem science research [J]. Chinese Journal of Applied Ecology, 2021, 32(2): 377-391.
[6]	FAN Zhen-zhen, WANG Xin, WANG Chao, BAI E. Effect of nitrogen and phosphorus addition on soil enzyme activities: A meta-analysis [J]. Chinese Journal of Applied Ecology, 2018, 29(4): 1266-1272.
[7]	WANG Li-qin1,2,3, QI Yu-chun1,2, DONG Yun-she1,2, PENG Qin1,2, GUO Shu-fang1,2,3, HE Yun-long1,2,3, YAN Zhong-qing1,2,3. Effects and mechanism of freeze-thawing cycles on key processes of nitrogen cycle in terrestrial ecosystem. [J]. Chinese Journal of Applied Ecology, 2015, 26(11): 3532-3544.
[8]	CHE Ming-liang1,2, CHEN Bao-zhang1, WANG Ying1,2, GUO Xiang-yun3. Review of dynamic global vegetation models (DGVMs). [J]. Chinese Journal of Applied Ecology, 2014, 25(1): 263-271.
[9]	LIU Shu-rong, HU Rong-gui, CAI Gao-chao. Effects of enhanced UV-B radiation on terrestrial ecosystem carbon cycle: A review. [J]. Chinese Journal of Applied Ecology, 2012, 23(07): 1992-1998.
[10]	MAO Liuxi¹; SUN Yanling^2,3; YAN Xiaodong². Modeling of carbon cycling in terrestrial ecosystem: A review [J]. Chinese Journal of Applied Ecology, 2006, 17(11): 2189-2195 .
[11]	YANG Jingcheng, HAN Xingguo, HUANG Jianhui, PAN Qingmin . Effects of land use change on carbon storage in terrestrial ecosystem [J]. Chinese Journal of Applied Ecology, 2003, (8): 1385-1390.
[12]	CHEN Qingqiang, SHEN Chengde, SUN Yanmin, YI Weixi, JIANG Mantao, PENG Shaolin, LI Zhi'an . Quantitative simulation on the vertical distribution of soil organic matters in mountainous soil profiles in the subtropical area, south China [J]. Chinese Journal of Applied Ecology, 2003, (8): 1239-1245.
[13]	YANG Jingcheng, HAN Xingguo, HUANG Jianhui, PAN Qingmin . Effects of land use change on carbon storage in terrestrial ecosystem [J]. Chinese Journal of Applied Ecology, 2003, (8): 1385-1390.
[14]	CHEN Qingqiang, SHEN Chengde, SUN Yanmin, YI Weixi, JIANG Mantao, PENG Shaolin, LI Zhi'an . Quantitative simulation on the vertical distribution of soil organic matters in mountainous soil profiles in the subtropical area, south China [J]. Chinese Journal of Applied Ecology, 2003, (8): 1239-1245.
[15]	GAO Quanzhou, TAO Zhen . Advances in studies on transported flux and properties of riverine organic carbon [J]. Chinese Journal of Applied Ecology, 2003, (6): 1000-1002.

Isolation and identification of a phenanthrene-degrading bacterial strain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments