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Isolation, purification, and phosphate-solubilizing capability of phosphorous bacteria in West Lake sediment

LI Wenhong;SHI Jiyan   

  1. Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China
  • Received:2005-10-27 Revised:2005-12-28 Online:2006-11-18 Published:2006-11-18

Abstract: By using solid culture media containing organic and inorganic phosphorus, six strains of phosphorous bacteria in West Lake sediment were isolated and purified,among which, two strains coded as OP1 and OP2 could decompose lecithin, and the other four coded as NOP1, NOP2, NOP3 and NOP4 could dissolve inorganic phosphate. OP1, OP2 and NOP3 had a stronger phosphate-solubilizing capability, followed by NOP4, while NOP1 and NOP2 lost this capability after isolation and purification. The water-soluble P concentration in the culture media inoculated with OP1,OP2 and NOP3 increased 38.53, 64.53 and 54.06 fold, respectively, compared with the control.

Key words: Phragmites communis, Clone plant, Ramet, Biomass allocation, Heterogeneous speed growth