Abstract: The calling and mating behaviors of Kumasia kumaso were studied in laboratory at (25±1) ℃ and (60±10) % RH under a cycle 14 L:10 D photo period, and the EAG response of male K. kumaso to female K. kumaso sex gland extracts was also tested. The calling and mating behaviors of K. kumaso could only be observed in scotophase. The females began calling at the first scotophase following emergence, and the peak of calling occurred during the second scotophase and decreased thereafter. The calling of the females commenced 0-4 h after dark, reached the maximum between the 5th and the 7th hour of the scotophase, and terminated during the last 1-2.5 h of the scotophase. Moth age had significant effects on the calling patterns. With increasing moth age, the onset time of calling advanced gradually, and the mean number of calling bouts as well as the calling length per bout had a gradual increase from calling day 1 to day 4 but decreased on day-5. The peak time and terminated time of calling advanced with increasing moth age. The mating of K. kumaso adults initiated during the first scotophase and terminated till the 5th scotophase following -emergence, and the peak of mating was observed during the second scotophase. The peak of mating was observed 5.5-7.0 h after dark, and advanced with increasing moth age. Moth age affected the mean onset time of mating and the copulation duration significantly. With increasing moth age, the mean onset time of mating advanced, and the copulation duration decreased. Sex ratio had significant effects on the mating behavior as well. Treatment 2♀:1♂ showed a significantly higher mating percentage, but an earlier onset time of mating and shorter copulation duration, as compared with treatment 1♀:1♂. The results of EAG test indicated that the male adults showed a significant EAG response to the sex gland extracts of 2-day-old virgin females.

Key words: Kumasia kumaso, calling behavior, mating behavior, rhythm, sex pheromone.