Abstract: A laboratory study was made on the calling and mating behaviors of adult Orthaga achatinaat (27±1) ℃, (60±10) % RH, and 14 L:10 D photoperiod.
 The female O. achatina did not call until the start of scotophase, with the calling percentage increased sharply at the 5^th hour and reached the peak
 at the 6^th-7^th hour of scotophase. The calling percentage varied with moth age, being the highest (>70%) at the age of 2-3 d. The mating behavior could be divided into two successive periods, i.e., calling period and mating period. The mating between female and male O. achatina occurred during the 5^th-9^th hour of scotophase, with the peak at the 6^th-7^th hour into scotophase, which was in accordance with the peak time of female calling behavior. Females mated only one time during their whole life. Treatment 1♀∶2♂showed a significantly higher mating percentage, but a significantly shorter mating duration time, compared with treatment 1♀∶1♂.

Key words: Orthaga achatina, calling behavior, mating behavior, stable isotope, cephalopods, trophic ecology, migration patterns.