Abstract: The determinations of super-cooling ability and body’s water and fat contents of overwintering Hyphantria cunea pupae showed that during overwin
tering, the female and male H. cunea pupae had the similar change patterns in their cold-resistance ability, i.e., increased with decreasing air temperature, but their body’s water and fat contents, super-cooling point (SCP), and freezing point (FP) differed at different overwintering stages. The SCP was significantly lower at preand mid overwintering stages than at late-overwintering stage, while FP had less difference among the stages. The body’s water content was significantly higher at mid-overwintering stage than at pre and late-over wintering stages. Comparing with nonoverwintering pupae, the overwintering pupae had significantly lower SCP and FP, but significantly higher body’s water and total fat contents. Regression analysis showed that there was a negative relationship between the water content and the SCP of female and male pupae (P<0.05).

Key words: Hyphantria cunea, overwintering pupae, body’s water content, fat content, supercooling point, Haizhou Bay, phytoplankton, community structure, species diversity, canonical correlation analysis (CCA).