Effects of illumination, moisture and medium on oospore production of Phytophthora boehmeriae

Abstract

Abstract: The effects of illumination, moisture and medium on the oospore production (OP) of Phytophthora boehmeriae were studied in vitro by three factors randomized block design. The results indicated that the order of the effects of the test factors was medium>illumination>moisture, and the effects of both medium and illumination were significant at 0.01 level.On the media tested, the number of formed oospore was decreased with the order of SLAmedium (SLA)>lima bean agar medium (LBA)>V₆ juice medium (V₆A)>clarified V₆ juice medium (V₆B). Of the three illumination conditions designed, OPfrom the continuous darkness treatment was the highest, next was 12h illumination +12h darkness, and the lowest was from the continuous illumination treatment.The interactive effects of the three factors on OP were all significant at 0.01 level. Among different combinations of illumination, moisture and medium, OPfrom low moisture+continuous darkness+SLA was the highest, and that from low moisture +continuous illumination+V₆Bwas the lowest.

Key words: Phytophthora boehmeriae, Oospore formation, Illumination, Moisture, Medium, cultivated barley, distribution characteristics, Qinghai-Tibet Plateau, β-glucan

Gao Zhimou, Zheng Xiaobo, Lu Jiayun . Effects of illumination, moisture and medium on oospore production of Phytophthora boehmeriae[J]. Chinese Journal of Applied Ecology, 1998, 9(3): 286-290.

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