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Chinese Journal of Applied Ecology ›› 1999, Vol. 10 ›› Issue (6): 739-742.

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Effect of La on Ca content in tobacco callus and oilseed rape seedling root

Zhang Jiarong, Gu Yuehua, Luo Jianping, Zhao Guiwen   

  1. University of Science and Technology of China, Hefei 230026
  • Received:1999-03-08 Revised:1999-06-03 Online:1999-11-25 Published:1999-11-25

Abstract: The Ca contents in tobacco callus and in its protoplasts were lower in La contained (0.01~0.10mmol·L-1) MSmedium than in the control (without La addition). As the culture time increased from 1 day to 30 days, the Ca contents declined rapidly. At low La concentration (0.01~0.05mmol·L-1), the Ca content in cell wall was higher than that of the control, and increased with increasing La concentration; while at high La concentration (0.10mmol·L-1), it decreased. Compared with Ce, La had a weaker effect on Ca exudation. After oilseed rape seeds were soaked with different concentrations of La(NO3)3 solution, the dynamics of Ca content in their seedling roots and protoplasts were the same as above mentioned. La made the tobacco callus quickly browned, and the increment of fresh weight was lower than that of the control.

Key words: La3+, Ce3+, Ca2+, Tobacco, Oilseed rape