Abstract: In this paper, the genetic relationships among six fruit fly species of Bactrocera in Yunnan Province of Southwest China, i. e. , B. tau, B. scutellaris, B. scutellata, B. cucurbitae, B. dorsalis and B. correcta, were studied by using the technique of random amplified polymorphic DNA (RAPD). Five primers with good repetition and high polymorphism were screened from 131 primers, and a total of 302 bands were amplified, among which, 111 bands were of genetic polymorphism. The primers OPC-01, OPI-17, OPL-07, OPL-08 and OPL-16 could be used for the identification of the 6 fruit fly species.

Key words: Agroforestry ecosystem, ³²P, Distribution, Translocation