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Chinese Journal of Applied Ecology ›› 2005, Vol. 16 ›› Issue (5): 967-970.

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Effects of culture condition on fatty acids in Pseudomonas sp

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  1. 1. Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China;
    2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
    3. First Institute Oceanography of SOA, Qingdao 266061, China
  • Received:2004-04-14 Revised:2004-08-02 Online:2005-05-15 Published:2005-05-15

Abstract: The investigation on the growth of Pseudomonas sp.and the dynamics of its fatty acids under various culture conditions indicated that the optimum temperature for its growth was 30 ℃,and the best carbon source was starch.13 kinds of fatty acids were detected,among which,c16:1(n-7),c15:0,c16:0,c17:0,c18:1(n-6),c18:1(n-9),9,10-cp-c17:0 and iso9,10-cp-c17:0 were the major ones.When cultured at 30 ℃,the relative content of unsaturated fatty acids decreased significantly.Carbon source in the media was the crucial factor affecting the contents of odd- and cyclopropane fatty acids.Cluster analysis revealed that temperature had a more obvious effect than carbon source.

Key words: Marine bacterium (Pseudomonas sp), Culture condition, Fatty acids

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