Detection of Bacillus thuringiensis plasmids and preliminary studiy on their functions

Abstract

Abstract: The plasmids carried in 11 Bacillus thuringiensis wild-type strains of 4 subspecies were detected using the improved lysozyme-alkaline preparation procedure,and an effective preparation result and recovery was found. By comparing the morphological, physiological and biochemical properties between wild-type strain YBT-1463 and its plasmid-free mutant strain BMB171, it was indicated that the plasmids of YBT-1463 carried insecticidal crystal protein genes,but no resistance genes. There was no relationship between resident plasmids and utilising of 19 sources of carbon and 12 sources of nitrogen in the wild-type strain YBT-1463.

Key words: Bacillus thuringiensis, Plasmid, Detection, Function

CLC Number:

Q343.4

LI Lin, WANG Zheng, YU Ziniu . Detection of Bacillus thuringiensis plasmids and preliminary studiy on their functions[J]. Chinese Journal of Applied Ecology, 2001, (6): 879-882.

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