欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报 ›› 1995, Vol. 6 ›› Issue (3): 317-322.

• 研究论文 • 上一篇    下一篇

株洲工业区土壤重金属污染与蚯蚓同工酶的研究

郭永灿1, 王振中1, 赖勤1, 张友梅1, 夏卫生1, 颜亨梅1, 邓继福2   

  1. 1. 湖南师范大学, 长沙 410006;
    2. 湖南省株洲市环境保护科学研究所, 株洲 412000
  • 收稿日期:1993-10-27 修回日期:1994-03-16 出版日期:1995-07-25 发布日期:1995-07-25
  • 基金资助:

    国家自然科学基金

Soil heavy metal pollution and earthworme isozyme

Guo Yongcan1, Wang Zhenzhong1, Lai Qin1, Zhang Youmei1, Xia Weisheng1, Yan Hengmei1, Deng Jifu2   

  1. 1. Hunan Normal University, Changsha 410006;
    2. Zhuzhou Instiute of Environmental Protection of Hunan Province, Zhuzhou 412000
  • Received:1993-10-27 Revised:1994-03-16 Online:1995-07-25 Published:1995-07-25

摘要: 用聚丙烯胺凝胶圆盘电泳法对蚯蚓酯酸及过氧化物酶同工酶进行比较研究,结果表明,重金属在蚯蚓体内富集量随污染程度增加而上升,重污染区为中污染区的2.27倍,为轻污染区的7.30倍。重污染区Cd、Ph、As在蚯蚓体内分别达到47.3、35.2、16.32μg·kg-1.其秩相关系数(rs)>0.66-0.77.蚯蚓酯酶同工酶酶带减少,酶活性降低,而其过氧化物酶酶带和酶活性有所增加。

关键词: 重金属污染, 白颈环毛蚓, 酯酶同工酶, 过氧化物酶同工酶, 酶活性, 空间异质性, 毛乌素沙漠-黄土高原过渡带, 土壤盐渍化, 影响因素

Abstract: Esterase and peroxidase isozymes of earthworms in polluted soils are studied with polyacrylamide gel disc electrophoresis.The results show that the heavy metal content in earthworms is increased with increasing pollution level, which is 2.27 and 7.30 times higher in heavy polluted soil than in medium and light polluted ones.The Cd, Ph and As contents of earthworms in heavy polluted soil are respectively 47.3, 35.2 and 16.32 μg·kg-1, and their order coefficient is more than 0.66-0.77.The bands and the activity of esterase isozyme are decreased, and those of peroxidase isozyme are increased.

Key words: Heavy metal pollution, Pheretima californica, Esterase isozyme, Peroxidase isozyme, Enzyme activity, soil salinization, Mu Us desert-loess plateau transition zone, spatial heterogeneity, influencing factors