长期氮水添加对温带草原土壤化学性质和微生物学特性的短期遗留效应

doi:10.13287/j.1001-9332.202307.016

摘要/Abstract

摘要： 据预测,内蒙古东部地区未来将出现氮沉降加剧和夏季降水增加的趋势,但大气氮沉降和降水增加都是不可持续的,增加强度可能减弱,也可能出现降低趋势。目前,历史氮、水输入停止后对生态系统的遗留效应尚不明确。本研究基于中国北方温带草原长达13年的氮、水添加实验平台,于处理第14年开始定向停止氮、水添加处理,探讨了历史氮、水添加在短期(2年)内对土壤理化性质和微生物学特性的遗留效应。结果表明: 停止添加两年后,历史氮添加对铵态氮、硝态氮、可溶性有机碳、氮和速效磷等土壤养分指标的正向遗留效应消失;但对大部分土壤微生物学特性指标仍存在遗留效应,历史15 g N·m^-2·a^-1添加处理微生物生物量碳、土壤呼吸和碱性磷酸单酯酶活性分别降低73.3%、81.9%和70.3%,表明氮输入停止后微生物学指标的恢复比土壤养分指标慢,具有迟滞效应。相关分析和冗余分析表明,氮对微生物学特性的负向遗留效应与对土壤pH的负向遗留效应有关。历史水添加在处理停止两年后,对土壤pH、铵态氮、可溶性有机碳、氮等以及土壤呼吸、酶活性等仍表现出显著的遗留效应,并与氮沉降的遗留效应存在交互作用。研究结果对预测局域环境改善条件下草地生态系统功能和服务变化趋势及揭示退化草地恢复机制具有重要意义。

关键词: 氮沉降, 降水增加, 遗留效应, 土壤酶活性, 微生物生物量

Abstract: Nitrogen deposition and summer precipitation in eastern Inner Mongolia are predicted to increase in recent decades. However, such increases in nitrogen inputs and precipitation may not be continuous under the future new patterns of global change, with the direction and magnitude of which may change or weaken. The legacy effects of nitrogen and water addition after cessation on ecosystems are still unclear. Based on a 13-year nitrogen and water addition experiment in temperate grassland of northern China, we examined the short-term (2 years) legacy effects of historical nitrogen and water addition on soil physicochemical properties and microbial properties after the cessation of nitrogen and water addition in the 14th year. The results showed that the positive effects of historical nitrogen addition on most of soil nutrient variables diminished after two years of cessation, including ammonium nitrogen, nitrate nitrogen, dissolved organic carbon and nitrogen, and Olsen-P concentrations. In contrast, there were legacy effects on soil microbial characteristics. For example, the historical nitrogen input of 15 g N·m^-2·a^-1 reduced microbial biomass carbon, respiration, and alkaline phosphomonoesterase activity by 73.3%, 81.9%, and 70.3% respectively. It implied that microbial parameters restored slowly in comparison with soil nutrients, showing a hysteresis effect. Results of Pearson’s correlation and redundancy analysis showed that the legacy effects of historical nitrogen addition on microbial parameters could be attributed to the negative effects of nitrogen addition on soil pH. Historical water addition showed significant legacy effects on soil pH, ammonium nitrogen, dissolved organic carbon and nitrogen, respiration, and soil enzyme activities, which significantly interacted with historical nitrogen addition. These results are of great significance to predict the changes in grassland ecosystem functions and services under the local environmental improvement conditions, and to reveal the restoration mechanism of degraded grassland.

Key words: nitrogen deposition, precipitation increment, legacy effect, soil enzyme activity, microbial biomass

代泽成, 刘月秀, 党宁, 王志瑞, 蔡江平, 张玉革, 宋永波, 李慧, 姜勇. 长期氮水添加对温带草原土壤化学性质和微生物学特性的短期遗留效应[J]. 应用生态学报, 2023, 34(7): 1834-1844.

DAI Zecheng, LIU Yuexiu, DANG Ning, WANG Zhirui, CAI Jiangping, ZHANG Yuge, SONG Yongbo, LI Hui, JIANG Yong. Short-term legacy effects of long-term nitrogen and water addition on soil chemical properties and micro-bial characteristics in a temperate grassland[J]. Chinese Journal of Applied Ecology, 2023, 34(7): 1834-1844.

参考文献

[1] Galloway JN, Dentener FJ, Capone DG, et al. Nitrogen cycles: Past, present, and future. Biogeochemistry, 2004, 70: 153-226
[2] LeBauer DS, Treseder KK. Nitrogen limitation of net primary productivity in terrestrial ecosystems is globally distributed. Ecology, 2008, 89: 371-379
[3] Cai J, Luo W, Liu H, et al. Precipitation-mediated responses of soil acid buffering capacity to long-term nitrogen addition in a semi-arid grassland. Atmospheric Environment, 2017, 170: 312-318
[4] Vitousek PM, Aber JD, Howarth RW, et al. Human alteration of the global nitrogen cycle: Sources and consequences. Ecological Applications, 1997, 7: 737-750
[5] IPCC. Climate Change 2013: The Physical Science Basis. Cambridge: Cambridge University Press, 2013
[6] Chen Q, Niu B, Hu Y, et al. Warming and increased precipitation indirectly affect the composition and turnover of labile-fraction soil organic matter by directly affecting vegetation and microorganisms. Science of the Total Environment, 2020, 714: 136787
[7] Wang X, Xu Z, Lü X, et al. Responses of litter decomposition and nutrient release rate to water and nitrogen addition differed among three plant species dominated in a semi-arid grassland. Plant and Soil, 2017, 418: 241-253
[8] Mason RE, Craine JM, Lany NK, et al. Evidence, causes, and consequences of declining nitrogen availability in terrestrial ecosystems. Science, 2022, 376: eabh3767
[9] Jia Y, Yu G, Gao Y, et al. Global inorganic nitrogen dry deposition inferred from ground- and space-based measurements. Scientific Reports, 2016, 6: 19810
[10] Yu G, Jia Y, He N, et al. Stabilization of atmospheric nitrogen deposition in China over the past decade. Nature Geoscience, 2019, 12: 424-429
[11] Liu F, Zhang Q, Zheng B, et al. Recent reduction in NOx emissions over China: Synthesis of satellite observations and emission inventories. Environmental Research Letters, 2016, 11: 114002
[12] Isbell F, Tilman D, Polasky S, et al. Low biodiversity state persists two decades after cessation of nutrient enrichment. Ecology Letters, 2013, 16: 454-460
[13] Clark CM, Hobbie SE, Venterea R, et al. Long-lasting effects on nitrogen cycling 12 years after treatments cease despite minimal long-term nitrogen retention. Glo-bal Change Biology, 2009, 15: 1755-1766
[14] Storkey J, Macdonald AJ, Poulton PR, et al. Grassland biodiversity bounces back from long-term nitrogen addition. Nature, 2015, 528: 401-404
[15] Power SA, Green ER, Barker CG, et al. Ecosystem recovery: Heathland response to a reduction in nitrogen deposition. Global Change Biology, 2006, 12: 1241-1252
[16] Sala OE, Gherardi LA, Reichmann L, et al. Legacies of precipitation fluctuations on primary production: Theory and data synthesis. Philosophical Transactions of the Royal Society B: Biological Sciences, 2012, 367: 3135-3144
[17] Grinath JB, Deguines N, Chesnut JW, et al. Animals alter precipitation legacies: Trophic and ecosystem engineering effects on plant community temporal dynamics. Journal of Ecology, 2018, 106: 1454-1469
[18] Meng Y, Li T, Liu H, et al. Legacy effects of nitrogen deposition and increased precipitation on plant productivity in a semi-arid grassland. Plant and Soil, 2022, doi: 10.1007/s11104-022-05550-x
[19] van der Heijden MGA, Bardgett RD, van Straalen NM. The unseen majority: Soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Eco-logy Letters, 2008, 11: 296-310
[20] Gershenson A, Bader NE, Cheng W. Effects of substrate availability on the temperature sensitivity of soil organic matter decomposition. Global Change Biology, 2009, 15: 176-183
[21] Tabatabai MA. Soil Enzymes. Methods of Soil Analysis: Part 2 Microbiological and Biochemical Properties. Madison WI, USA: Soil Science Society of America,1994, 5: 775-833
[22] Li H, Xu Z, Yang S, et al. Responses of soil bacterial communities to nitrogen deposition and precipitation increment are closely linked with aboveground community variation. Microbial Ecology, 2016, 71: 974-989
[23] Yang S, Xu Z, Wang R, et al. Variations in soil microbial community composition and enzymatic activities in response to increased N deposition and precipitation in Inner Mongolian grassland. Applied Soil Ecology, 2017, 119: 275-285
[24] Zhu J, Chen Z, Wang Q, et al. Potential transition in the effects of atmospheric nitrogen deposition in China. Environmental Pollution, 2020, 258: 113739
[25] Chen J, Luo Y, Xia J, et al. Differential responses of ecosystem respiration components to experimental warming in a meadow grassland on the Tibetan Plateau. Agricultural and Forest Meteorology, 2016, 220: 21-29
[26] Zhang T, Chen HYH, Ruan H. Global negative effects of nitrogen deposition on soil microbes. ISME Journal, 2018, 12: 1817-1825
[27] Wang Z, Yang S, Wang R, et al. Compositional and functional responses of soil microbial communities to long-term nitrogen and phosphorus addition in a calcareous grassland. Pedobiologia, 2020, 78: 150612
[28] Xu ZW, Li MH, Zimmermann NE, et al. Plant functional diversity modulates global environmental change effects on grassland productivity. Journal of Ecology, 2018, 106: 1941-1951
[29] Treseder KK. Nitrogen additions and microbial biomass: A meta-analysis of ecosystem studies. Ecology Letters, 2008, 11: 1111-1120
[30] Treseder KK. A meta-analysis of mycorrhizal responses to nitrogen, phosphorus, and atmospheric CO₂ in field studies. New Phytologist, 2004, 164: 347-355
[31] Xu H, Qu Q, Li G, et al. Impact of nitrogen addition on plant-soil-enzyme C-N-P stoichiometry and microbial nutrient limitation. Soil Biology and Biochemistry, 2022, 174: 108834
[32] Wang R, Liu H, Sardans J, et al. Interacting effects of urea and water addition on soil mineral-bound phospho-rus dynamics in semi-arid grasslands with different land-use history. European Journal of Soil Science, 2021, 72: 946-962
[33] Li J, Sang C, Yang J, et al. Stoichiometric imbalance and microbial community regulate microbial elements use efficiencies under nitrogen addition. Soil Biology and Biochemistry, 2021, 156: 108207
[34] Sun S, Zhao H, Xing F, et al. Response of soil micro-bial community structure to increased precipitation and nitrogen addition in a semiarid meadow steppe. European Journal of Soil Science, 2017, 68: 524-536