杉木种植和磷添加对土壤微生物生物量及胞外酶活性的影响

doi:10.13287/j.1001-9332.202303.003

应用生态学报 ›› 2023, Vol. 34 ›› Issue (3): 631-638.doi: 10.13287/j.1001-9332.202303.003

杉木种植和磷添加对土壤微生物生物量及胞外酶活性的影响

豆梦珂¹, 张伟东^2,3, 杨庆朋^2,3, 陈龙池^2,3, 刘晔嘉⁴, 胡亚林^1*

¹福建农林大学林学院森林生态-稳定同位素研究中心, 福州 350002;
²中国科学院沈阳应用生态研究所/中国科学院森林生态与管理重点实验室/会同森林生态实验站, 沈阳 110016;
³亚热带森林生态系统结构与服务功能湖南省重点实验室, 湖南会同 418307;
⁴湖南省沅陵县林业局, 湖南沅陵 419699

收稿日期:2022-10-24 接受日期:2022-12-28 发布日期:2023-09-15
通讯作者: *E-mail: huyl@iae.ac.cn
作者简介:豆梦珂, 女, 1996年生, 硕士研究生。主要从事森林培育技术研究。E-mail: dmk15836@163.com
基金资助:
国家重点研发计划项目(2021YFD2201301-04,2022YFF1303003)和国家自然科学基金促进海峡两岸科技合作联合基金重点项目(U1805243)资助。

Effects of Chinese fir planting and phosphorus addition on soil microbial biomass and extracellular enzyme activities.

DOU Mengke¹, ZHANG Weidong^2,3, YANG Qingpeng^2,3, CHEN Longchi^2,3, LIU Yejia⁴, HU Yalin^1*

¹Forest Ecology & Stable Isotope Center, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
²Institute of Applied Ecology, Chinese Academy of Sciences/Key Laboratory of Forest Ecology and Management, Chinese Academy of Sciences/Huitong Experimental Station of Forest Ecology, Shenyang 110016, China;
³Hunan Key Laboratory for Structure and Ecosystem Service of Subtropical Forest, Huitong 418307, Hunan, China;
⁴Forestry Department of Yuanling County, Yuanling 419699, Hunan, China

Received:2022-10-24 Accepted:2022-12-28 Published:2023-09-15

摘要/Abstract

摘要： 植物可以通过凋落物和根系分泌物改变土壤微生物生物量及碳氮磷转化胞外酶活性,进而影响土壤碳氮磷循环过程。然而,针对土壤磷有效性如何影响植物与土壤微生物之间的关系依然不清楚。本研究在不同磷添加水平下(0、1.95、3.9、7.8、15.6 g P·m^-2·a^-1),分析杉木种植对土壤微生物生物量和碳氮磷转化胞外酶活性的影响。结果表明: 杉木种植显著改变了土壤微生物生物量与碳氮磷转化胞外酶活性,而且影响程度受磷添加水平的调控。在不添加磷处理下,种植杉木显著降低了土壤养分有效性,并引起土壤酸化,导致磷限制加剧,抑制土壤微生物生物量。磷添加逐渐减缓磷限制,随着磷添加水平的升高(1.95、3.9、7.8、15.6 g P·m^-2·a^-1)土壤酸性磷酸酶活性比对照分别降低30.0%、30.5%、35.3%和47.1%。在3个磷添加水平处理下(1.95、3.9、7.8 g P·m^-2·a^-1),杉木对土壤微生物生长的抑制作用得到缓解。在高水平磷添加处理下(15.6 g P·m^-2·a^-1),由于磷添加引起的氮养分限制,杉木种植对土壤微生物再次呈抑制作用。杉木种植和土壤磷有效性共同调控土壤微生物生物量与胞外酶活性,改变磷限制。

关键词: 磷添加, 杉木种植, 土壤微生物生物量, 胞外酶活性, 养分限制

Abstract: Plants can alter soil microbial biomass and extracellular enzyme activities related with carbon (C), nitrogen (N), and phosphorus (P), through litter and root exudates, with consequences on soil carbon, nitrogen and phosphorus (P) cycling. However, it is not well known how the changes in soil phosphorus availability affect the relationships between plants and soil microorganisms. In this study, a factorial experiment was conducted to investigate the effects of Chinese fir (Cunninghamia lanceolata) planting and different levels of P addition (0, 1.95, 3.9, 7.8 and 15.6 g P·m^-2·a^-1) on soil microbial biomass and extracellular enzyme activities. The results showed that planting Chinese fir planting significantly altered soil microbial biomass and C- and N- and P-related extracellular enzyme activities, but the effects were dependent on P addition levels. Without P addition, Chinese fir planting significantly reduced soil nutrient availability and pH, which led to the aggravation of P limitation and lower soil microbial biomass. P addition relieved P limitation, and reduced soil acid phosphatase (ACP) activities by 30.0%, 30.5%, 35.3% and 47.1% with the increasing P addition level (1.95, 3.9, 7.8 and 15.6 g P·m^-2·a^-1). Under three P addition levels (1.95, 3.9 and 7.8 g P·m^-2·a^-1), the negative effects of Chinese fir planting on soil microbial growth were alleviated. Under the high P addition level (15.6 g P·m^-2·a^-1), the negative effects of Chinese fir planting on soil microbial growth occurred again due to soil N limitation. Taken together, Chinese fir planting and soil P availability generally affected soil microbial biomass and extracellular enzyme activities, and changed P limitation.

Key words: phosphorus addition, Chinese fir planting, soil microbial biomass, extracellular enzyme activity, nutrient limitation

豆梦珂, 张伟东, 杨庆朋, 陈龙池, 刘晔嘉, 胡亚林. 杉木种植和磷添加对土壤微生物生物量及胞外酶活性的影响[J]. 应用生态学报, 2023, 34(3): 631-638.

DOU Mengke, ZHANG Weidong, YANG Qingpeng, CHEN Longchi, LIU Yejia, HU Yalin. Effects of Chinese fir planting and phosphorus addition on soil microbial biomass and extracellular enzyme activities.[J]. Chinese Journal of Applied Ecology, 2023, 34(3): 631-638.

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杉木种植和磷添加对土壤微生物生物量及胞外酶活性的影响

Effects of Chinese fir planting and phosphorus addition on soil microbial biomass and extracellular enzyme activities.

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